Computed tomography of testicular torsion in a juvenile dog with unilateral cryptorchidism

A 14-week-old male unilaterally cryptorchid Clumber spaniel was presented for acute lethargy. Physical examination revealed abdominal pain, and a single testis was palpated in the scrotum. Abdominal ultrasound and computed tomography (CT) revealed a poorly vascularized, ovoid structure immediately caudal to the left kidney with scant regional peritoneal effusion. Left intra-abdominal testicular torsion was confirmed at surgery, and routine cryptorchidectomy was performed. The patient recovered uneventfully from anesthesia and surgery.Key clinical message:The most common CT characteristics of testicular torsion were present in this case and correlated well with sonographic findings to allow for rapid, accurate diagnosis and surgical planning of unilateral, non-neoplastic, intra-abdominal cryptorchid testicular torsion in a juvenile dog. Contrast enhanced CT facilitated accurate localization of the undescended testis and evaluation of testicular perfusion and may be a useful alternative to ultrasound for diagnosing testicular torsion, especially in indeterminate cases.     

Differential and successive effects of residue quality and soil mineral N on water-stable aggregation during crop residue decomposition

Residue quality has been shown to influence soil water-stable aggregation (WSA) during crop residue decomposition, but there is still little information about its interactive effect with soil mineral N availability. The aim of this study was to determine the effect of soil mineral N on WSA during the decomposition of two high-C/N crop residues (wheat straw with C/N = 125.6 and miscanthus straw with C/N = 311.3). The two crop residues were combined with three mineral N addition rates (0, 60, and 120 mg N kg⁻¹ dry soil). Respiration, soil mineral N content, and WSA (expressed as mean-weight diameter, MWD) were measured on several dates during a 56-d incubation. The effect of decomposing crop residues on WSA followed two phases. (i) Between 0 and 7 d, the increase in WSA was related to intrinsic residue quality with higher decomposability of the wheat straw resulting in higher WSA. (ii) Thereafter, and until the end of the experiment, mineral N addition rates had a predominant but negative influence on WSA. In this second phase, the average MWD of residue-treated soils was 0.92, 0.55, and 0.44 mm for the 0, 60 and 120 mg N kg⁻¹ dry soil addition rates, respectively. Mineral N addition which did result in higher crop residue decomposition did not lead to higher WSA. WSA during crop residue decomposition is therefore not simply positively related to the induced microbial activity, and changes in microbial community composition with differential effects on WSA must be involved. The impact of high-C/N crop residues inputs on WSA, initially assumed to be low, could actually be strong and long-lasting in situations with low soil mineral N content.     

Investigating flow mechanisms in a forest soil by mixed‐effects modelling

Uniform and preferential flow produces typical infiltration patterns. We made three tracer experiments in a Norway spruce forest soil and qualitatively identified the dominant flow regime based on stained patterns. We analysed soil texture, fine root density and soil bulk density from preferential flow paths and the soil matrix by means of linear mixed‐effects models. These models can account for dependences in the data structure caused by hierarchical sampling and can deal with missing values. There were between 44% (topsoil) and 76% (subsoil) larger root densities in preferential flow paths than in the soil matrix. No significant differences in soil texture were detected. The bulk density was greater in the soil matrix by 0.12 g cm⁻³, which is probably because of a greater soil organic matter content of preferential flow paths. Using flow patterns and model results we identified the dominant flow mechanisms. At this study site, roots constituted the main preferential flow paths and induced macropore flow, especially in the topsoil. In the subsoil, root density decreased and inhomogeneous infiltration from preferential flow paths into the soil matrix caused unstable flow.     

Effect of feeding intensity and milking system on nutritionally relevant milk components in dairy farming systems in the North East of England

There is increasing concern that the intensification of dairy production reduces the concentrations of nutritionally desirable compounds in milk. This study therefore compared important quality parameters (protein and fatty acid profiles; α-tocopherol and carotenoid concentrations) in milk from four dairy systems with contrasting production intensities (in terms of feeding regimens and milking systems). The concentrations of several nutritionally desirable compounds (β-lactoglobulin, omega-3 fatty acids, omega-3/omega-6 ratio, conjugated linoleic acid c9t11, and/or carotenoids) decreased with increasing feeding intensity (organic outdoor ≥ conventional outdoor ≥ conventional indoors). Milking system intensification (use of robotic milking parlors) had a more limited effect on milk composition, but increased mastitis incidence. Multivariate analyses indicated that differences in milk quality were mainly linked to contrasting feeding regimens and that milking system and breed choice also contributed to differences in milk composition between production systems.     

Effects of Broomrape Parasitism on Sunflower Plants: Growth,Development, and Mineral Nutrition

Sunflower broomrape (Orobanche cumana Wallr.) is a parasitic plant that infects sunflower (Helianthus annuus L.) plants. In this work, sunflower plants were grown under greenhouse conditions in pots with the substrate infested or non-infested with broomrape seeds. At different numbers of days after sowing, plant height, internode lengths, number of leaves, head diameter, mineral composition of leaves, and potassium (K) concentration in stem were measured. The negative effects of broomrape parasitism were assessed from 57 d after sowing, when broomrape started to emerge. Parasitized plants exhibited lower shoot dry weight, height, and head diameter than control plants. The reduction in internode lengths was associated with a decrease in the gradient of K concentration from basal to apical stem. The mineral composition of leaves was also affected in parasitized plants. The concentrations of calcium (Ca), magnesium (Mg), manganese (Mn), and zinc (Zn) in leaves of parasitized plants were lower than those of the control plants, while there were few differences for K, phosphorus (P), iron (Fe), and copper (Cu). The effects of parasitism are discussed in relation to their competition for resources and to perturbations of the host physiology such as hormonal and water balance.     

Soil fertility ‐ crop genotype associations and interactions 1

This paper discusses the opportunities to combine the efforts of plant geneticists and soil scientists to develop crop varieties adapted to the existing soil conditions and techniques used for production. Such research should result in improved varieties for the best as well as poorest of soil and production situations.     

Sensitivity of soft red winter wheat cultivars to chlorate‐induced toxicity

The use of chlorate as a nitrate analogue to screen soft red winter wheat (Triticum aestivum L.) cultivars for differences in nitrate reductase activity (NRA) was studied by adding potassium chlorate to a hydroponic nutrient solution in which wheat seedlings were growing. After 14 days, leaf symptoms indicating chlorate‐induced toxicity were rated. It was hypothesized that wheat plants which were susceptible to chlorate‐induced toxicity reduced chlorate and nitrate more rapidly than did resistant plants. In experiments testing the potential of this assay, wheat and barley (Hordeum vulgare L.) cultivars previously reported to have low NRA were less susceptible to chlorate‐induced toxicity than were cultivars reported to have high NRA. The assay was used to screen 15 soft red winter wheat cultivars for differences in sensitivity to chlorate‐induced toxicity. Variable toxic reactions were observed both among and within the cultivars. To determine whether the within‐cultivar variation was environmental or genetic, single plant selections for contrasting chlorate response were made, and bulked progeny were rescreened. In eight of 15 cultivars, the contrasting selections were different for chlorate‐induced toxic response, indicating heterogeneity for this trait within these eight cultivars. These chlorate‐selected lines may also be near‐isogenic lines for NRA. Seedling screening of wheat for chlorate response may be useful for identification of high NRA breeding lines.     

Influence of root restriction on two cultivars of summer squash (Cucurbita pepo L.)

Variable root restricting conditions were imposed on summer squash (Cucurbita pepo L.) cultivars ‘Senator’ and Dixie’ using container sizes of 0.35, 2.00, and 7.60 liters. Nondestructive and destructive plant sampling were conducted to evaluate leaf area production, dry weight accumulation and partitioning, crop growth rate and net assimilation rate, and flower and fruit development. Within 10 days after transplanting (DAT), ‘Senator’ exhibited declining leaf area production under increased root restriction, and within 17 DAT leaf area was diminished for both cultivars under increased root restricting conditions. Dry weight accumulation in leaves, stems, roots, and fruit was reduced for both cultivars over time and level of root restriction severity. There was a tendency for greater vegetative growth reductions with ‘Senator’ than with ‘Dixie’ at comparable levels of root restriction. Root‐to‐shoot ratio, timing and duration of flowering, and sex of flowers were not significantly impacted by root restriction level or cultivar. Fruit dry weight was greatest for Dixie’ early on, but by 28 DAT, total fruit dry weight produced did not differ for the cultivars except under the most severe root restricting conditions. Fruit dry weight production was limited for both cultivars as container volume became smaller.     

Seasonal dynamics of mineral nutrients and carbohydrates by walnut tree leaves

The dry weight accumulation per leaf as well as the concentration per gram of dry weight and the accumulation of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) were determined in walnut tree leaves (Juglans regia L.) during a complete life cycle. Additionally, the dynamics of plant nutrient concentration in leaf petiole sap and carbohydrate accumulation in leaves were studied in relation to the main life cycle events of the walnut tree. Total N, P, K, Cu, and Zn concentrations decreased, whereas that of Ca, Mg, and Mn increased during the season. Iron concentration fluctuated around a mean value. Total N, P, K, Mg, and Cu concentrations detected in younger mature leaves were at the sufficient level, whereas Ca, Fe, Mn, and Zn concentrations were at higher levels as compared to those previously reported. All the detected nutrient accumulations increased abruptly during leaf ontogeny and leaf maturation until a maximum level was attained in the younger mature leaves. Similarly, sucrose, glucose, and fructose accumulation were observed at the same period. The rates of total N, P, Cu, and Zn accumulation were lower than the rates of the observed dry matter accumulation and nutrient concentration dilution. Potassium and Mn accumulation rates were almost equal, whereas those for Ca and Mg were higher as compared to the dry matter accumulation rate. The fast embryo growing phase resulted in a considerable decrease in dry weight, total N, P, K, Cu, Zn, and carbohydrate accumulation, and to a lesser degree in Ca, Mg, and Mn accumulation. Nutrient accumulation reduction in leaves by the influence of the growing fruits were estimated to be: total N 52%, K 48%, P 29.5%, Mg 16.3%, Ca 15%, Fe 51.2%, Cu 55.2%, Zn 37.3%, and Mn 5.4% of the maximum nutrient value of the younger mature leaves. Old leaves preserved nutrients before leaf fall as follows: total N 25.4%, P 45%, K 31%, Ca 74.8%, Mg 76.5%, Mn 89.2%, Fe and Zn 50%, and Cu 37%. Nutrient remobilization from the senescing old leaves before leaf fall were: total N 22.6%, P 25.5%, K 21%, Ca 10.2%, Mg 7%, Fe 3.2%, Mn 5.4%, Cu 8%, and Zn 13.3% of the maximum value in the younger mature leaves. In early spring, the absorption rates of N, P, and Ca were low while those of Mg, Fe, Mn, Cu, and Zn were high. During the fast growing pollen phase, the N, P, Fe, Mn, Cu, and Zn concentrations were reduced. Calcium concentration is supposed to be more affected by the rate of transpiration rather than during the growing of embryo. Calcium and Mg concentrations in the sap were negatively correlated. The detected K concentration level in the sap was as high as 33 to 50 times that of soluble N, 12 to 21 times to that of P, 5 times to that of Ca, and 10 to 20 times to that of Mg. The first maximum of starch accumulation in mature leaves was observed during the slow growing embryo phase and a second one after fruit ripening. Old senescing leaves showed an extensive carbohydrate depletion before leaf fall.