全文获取类型
收费全文 | 140636篇 |
免费 | 7717篇 |
国内免费 | 89篇 |
专业分类
林业 | 5639篇 |
农学 | 4258篇 |
基础科学 | 939篇 |
16253篇 | |
综合类 | 24384篇 |
农作物 | 5319篇 |
水产渔业 | 6712篇 |
畜牧兽医 | 74287篇 |
园艺 | 1596篇 |
植物保护 | 9055篇 |
出版年
2018年 | 1788篇 |
2017年 | 1982篇 |
2016年 | 1848篇 |
2015年 | 1592篇 |
2014年 | 1994篇 |
2013年 | 5234篇 |
2012年 | 3590篇 |
2011年 | 4405篇 |
2010年 | 2854篇 |
2009年 | 2975篇 |
2008年 | 4369篇 |
2007年 | 4114篇 |
2006年 | 3983篇 |
2005年 | 3643篇 |
2004年 | 3509篇 |
2003年 | 3611篇 |
2002年 | 3372篇 |
2001年 | 4350篇 |
2000年 | 4359篇 |
1999年 | 3377篇 |
1998年 | 1355篇 |
1997年 | 1424篇 |
1996年 | 1350篇 |
1995年 | 1667篇 |
1994年 | 1458篇 |
1993年 | 1415篇 |
1992年 | 2985篇 |
1991年 | 3254篇 |
1990年 | 3089篇 |
1989年 | 3113篇 |
1988年 | 2931篇 |
1987年 | 2983篇 |
1986年 | 3109篇 |
1985年 | 2940篇 |
1984年 | 2449篇 |
1983年 | 2106篇 |
1982年 | 1454篇 |
1979年 | 2256篇 |
1978年 | 1822篇 |
1977年 | 1642篇 |
1976年 | 1575篇 |
1975年 | 1640篇 |
1974年 | 2083篇 |
1973年 | 2151篇 |
1972年 | 2045篇 |
1971年 | 1907篇 |
1970年 | 1837篇 |
1969年 | 1702篇 |
1968年 | 1472篇 |
1967年 | 1555篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
Horak IG Braack LE Fourie LJ Walker JB 《The Onderstepoort journal of veterinary research》2000,67(4):239-250
Ixodid ticks were collected from 104 wild carnivores belonging to 23 species in various nature reserves and on several farms in all nine provinces of South Africa. Seven feral cats in a nature reserve were also examined. Twenty-four tick species belonging to seven genera were recovered and identified. Amongst these ticks we consider the adults of Haemaphysalis leachi, Haemaphysalis spinulosa, Haemaphysalis zumpti, Ixodes rubicundus, Rhipicentor nuttalli, Rhipicephalus simus and Rhipicephalus turanicus to be true parasites of wild carnivores. Although numerous adult Rhipicephalus appendiculatus and Rhipicephalus zambeziensis were collected from some lions these were either sick or old animals. The immature stages of seven species regularly utilized wild carnivores as hosts on an opportunistic basis. 相似文献
992.
D Jordan S A McEwen A M Lammerding W B McNab J B Wilson 《Preventive veterinary medicine》1999,41(1):37-54
A Monte Carlo simulation model was constructed for assessing the quantity of microbial hazards deposited on cattle carcasses under different pre-slaughter management regimens. The model permits comparison of industry-wide and abattoir-based mitigation strategies and is suitable for studying pathogens such as Escherichia coli O157:H7 and Salmonella spp. Simulations are based on a hierarchical model structure that mimics important aspects of the cattle population prior to slaughter. Stochastic inputs were included so that uncertainty about important input assumptions (such as prevalence of a human pathogen in the live cattle-population) would be reflected in model output. Control options were built into the model to assess the benefit of having prior knowledge of animal or herd-of-origin pathogen status (obtained from the use of a diagnostic test). Similarly, a facility was included for assessing the benefit of re-ordering the slaughter sequence based on the extent of external faecal contamination. Model outputs were designed to evaluate the performance of an abattoir in a 1-day period and included outcomes such as the proportion of carcasses contaminated with a pathogen, the daily mean and selected percentiles of pathogen counts per carcass, and the position of the first infected animal in the slaughter run. A measure of the time rate of introduction of pathogen into the abattoir was provided by assessing the median, 5th percentile, and 95th percentile cumulative pathogen counts at 10 equidistant points within the slaughter run. Outputs can be graphically displayed as frequency distributions, probability densities, cumulative distributions or x-y plots. The model shows promise as an inexpensive method for evaluating pathogen control strategies such as those forming part of a Hazard Analysis and Critical Control Point (HACCP) system. 相似文献
993.
994.
The sudden death of two horses was attributed to the rapid and acute development of pulmonary aspergillosis. One horse was making excellent postoperative progress after a jejunal resection and anastomosis for intestinal adhesions. The other horse was being treated routinely for equine protozoal myeloencephalitis (EPM). Signs of fever and an increased respiratory rate were detected shortly before death in the first horse, but no premonitory clinical signs characteristic of pulmonary infection were detected in the horse being treated for EPM. Both horses developed rapidly debilitating, acute pulmonary mycosis and died unexpectedly. 相似文献
995.
996.
P J Felsburg B J Hartnett P S Henthorn P F Moore S Krakowka H D Ochs 《Veterinary immunology and immunopathology》1999,69(2-4):127-135
Canine X-linked severe combined immunodeficiency (XSCID) is due to mutations in the common gamma (gamma c) subunit of the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. The most striking clinical feature is a failure to thrive or 'stunted' growth. Recurrent or chronic infections begin at the time of decline of maternal antibody, usually between six and eight weeks of age. Affected dogs rarely survive past three to four months of age. The major pathologic feature of canine XSCID is a small, dysplastic thymus. Grossly identifiable lymph nodes, tonsils, and Peyer's patches are absent in XSCID dogs. During the neonatal period, XSCID dogs have few, if any, peripheral T cells and increased number of peripheral B cells. Some XSCID dogs do develop phenotypically mature, nonfunctional T cells with age, however, the absolute number of peripheral T cells remain significantly decreased compared to age-matched normal dogs. An interesting finding is that as soon as T cells begin to appear in XSCID dogs they rapidly switch from a CD45RA+ (naive) phenotype to a CD45RA- (activated or memory phenotype). One of the characteristic findings in XSCID dogs is an absent or markedly depressed blastogenic response of T cells in response to stimulation through the T cell receptor and when the necessary second messengers for cellular proliferation are directly provided that by-pass signals delivered through ligand-receptor interaction. The proliferative defect is due to the inability of T cells to express a functional IL-2 receptor. Canine XSCID B cells do not proliferate following stimulation with T cell-dependent B cell mitogens, however, they proliferate normally in response to T cell-independent B cell mitogens. Canine XSCID B cells are capable of producing IgM but are incapable of class-switching to IgG antibody production following immunization with the T cell-dependent neoantigen, bacteriophage phiX174. The number of thymocytes in the XSCID thymus is approximately 0.3% of the thymocytes present in the thymus of age-matched normal dogs. The proportion of CD4-CD8- thymocytes in XSCID dogs is increased 3.5-fold and the CD4+CD8+ population is decreased 2.3-fold. These findings demonstrate that (1) a functional gamma c is required for normal B and T cell function, (2) early T cell development is highly dependent upon a functional gamma c, and (3) B cell development can occur through a gamma c-independent pathway. 相似文献
997.
Viruses may be viewed as genetic information whose success depends on avoiding elimination from individual hosts, or, if this is not possible, in persisting in the population of their hosts. The immune system represents the crucial defense mechanism responsible for the elimination of viruses from individual hosts and for the establishment of immunity that prevents a recurring infection by the same virus. Herd immunity, i.e., immunity of the population against infection resulting from the immunity of a certain fraction of the individuals of the population, represents an important concept in the interaction of viruses with their hosts. Thus, if the number of susceptible hosts decreases below a critical threshold, viruses may risk extinction because they literally run out of substrate. This possibility is increased due to the viruses' low resistance to inactivation outside their hosts by physical influences, such as heat and ultraviolet radiation. Some viruses have adopted a strategy of dual host tropism, i.e., they may reside in reservoir hosts that permit them to survive for extended periods of times. Examples of such viruses are the large and taxonomically diverse group of arboviruses. Moreover, although not normally discussed under this aspect, influenza viruses can also be said to have adopted this strategy, in view of water fowl representing reservoir hosts from which complete viruses may directly cross over to mammals, as was the case with the equine Jilin (Guo et al., 1995) or, more recently, the H5 subtype of influenza virus in humans (Shortridge et al., 1998). In addition, influenza viruses of birds may be transmitted, albeit only partially, through genetic reassortment (Shu et al., 1996). 相似文献
998.
OBJECTIVE: To evaluate effects of butorphanol tartrate and buprenorphine hydrochloride on withdrawal threshold to a noxious stimulus in conscious African grey parrots. ANIMALS: 29 African grey parrots (Psittacus erithacus erithacus and Psittacus erithacus timneh). PROCEDURE: Birds were fitted with an electrode on the medial metatarsal region of the right leg, placed into a test box, and allowed to acclimate. An electrical stimulus (range, 0.0 to 1.46 mA) was delivered to each bird's foot through an aluminum perch. A withdrawal response was recorded when the bird lifted its foot from the perch or vigorously flinched its wings. Baseline threshold to a noxious electrical stimulus was determined. Birds then were randomly assigned to receive an i.m. injection of saline (0.9% NaCl) solution, butorphanol (1.0 mg/kg of body weight), or buprenorphine (0.1 mg/kg), and threshold values were determined again. RESULTS: Butorphanol significantly increased threshold value, but saline solution or buprenorphine did not significantly change threshold values. CONCLUSIONS AND CLINICAL RELEVANCE: Butorphanol had an analgesic effect, significantly increasing the threshold to electrical stimuli in African grey parrots. Buprenorphine at the dosage used did not change the threshold to electrical stimulus. Butorphanol provided an analgesic response in half of the birds tested. Butorphanol would be expected to provide analgesia to African grey parrots in a clinical setting. 相似文献
999.
S D Sorden P A Harms P Nawagitgul D Cavanaugh P S Paul 《Journal of veterinary diagnostic investigation》1999,11(6):528-530
An immunohistochemical method for the detection of type 2 porcine circovirus (PCV2) in paraffin-embedded tissue was developed. Rabbits were inoculated with purified PCV2 to obtain a polyclonal antiserum. Antiserum was applied to sections of porcine tissue that contained lesions consistent with postweaning multisystemic wasting syndrome and in which PCV2 genome had been demonstrated by in situ hybridization. In all cases (18/18), the density and distribution of positive cells detected by in situ hybridization or immunohistochemistry were identical. The immunohistochemical method is more rapid and less expensive than in situ hybridization and is thus more suitable for routine diagnostic use. 相似文献
1000.
The possibility of using the polymerase chain reaction (PCR) for the detection of fowl adenoviruses (FAdV) was tested. The optimal reaction parameters were evaluated and defined for purified genomic DNA of type 8 fowl adenovirus (FAdV-8), and then the same conditions were applied for nucleic acid extracted from infected cells. One hundred picograms of purified viral DNA, or 250 FAdV-8-infected cells, were detected by ethidium bromide staining of the PCR products in agarose gels. The sensitivity was increased to 10 pg purified viral DNA, or 25 infected cells, when the PCR products were hybridized with a specific labeled probe. Several field isolates of FAdV and the CELO virus (FAdV serotype 1) could be amplified by the same primers and conditions, but the size of the amplicons was smaller than that for the FAdV-8 PCR product. Other avian viruses and uninfected cell cultures tested negative. 相似文献