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Techniques to monitor honey bee (Apis mellifera) egg production in cages allow researchers to study how different environmental factors contribute to reproduction. However, although the conditions required to facilitate queen egg production in a laboratory setting have been established, limited work has addressed the requirements for stimulating and monitoring worker egg laying. Here, we documented that drone laying workers will lay eggs in Queen Monitoring Cages (QMC), specialized cages designed to facilitate queen egg laying under controlled conditions. Egg production and worker mortality were compared between QMCs containing queens and those containing drone laying workers. High-definition images of the last abdominal segments of living first-instar larvae hatched from worker laid eggs and those putatively laid by queens were qualitatively compared to identify candidate characteristics to determine their sex.  相似文献   
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A 14-week-old male unilaterally cryptorchid Clumber spaniel was presented for acute lethargy. Physical examination revealed abdominal pain, and a single testis was palpated in the scrotum. Abdominal ultrasound and computed tomography (CT) revealed a poorly vascularized, ovoid structure immediately caudal to the left kidney with scant regional peritoneal effusion. Left intra-abdominal testicular torsion was confirmed at surgery, and routine cryptorchidectomy was performed. The patient recovered uneventfully from anesthesia and surgery.Key clinical message:The most common CT characteristics of testicular torsion were present in this case and correlated well with sonographic findings to allow for rapid, accurate diagnosis and surgical planning of unilateral, non-neoplastic, intra-abdominal cryptorchid testicular torsion in a juvenile dog. Contrast enhanced CT facilitated accurate localization of the undescended testis and evaluation of testicular perfusion and may be a useful alternative to ultrasound for diagnosing testicular torsion, especially in indeterminate cases.  相似文献   
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Effects of varying the proportions of NO3 and NH4+ in the growth medium on seedling growth and tomato fruit yield (Lycopersicon esculentum L. cv. Trust F1) were investigated in greenhouse hydroponic experiments. The presence of NH4+ as the sole N source (11 mM) was toxic: it curtailed growth and decreased chlorophyll content of the leaves. However, at low concentration (10 % of total N), the presence of NH4+, with or without added dissolved inorganic carbon (DIC), increased vegetative growth and fruit yield by ˜ 15 %, and enhanced taste/flavor of the fruits. In DIC‐enriched treatment, pH was maintained at 5.8 by addition of KHCO3 or as CaCO3. The presence of NH4+, at 10 % of total N, inhibited NO3 uptake rates by ˜ 27 %. The rates of uptake of NO3 and NH4+ were comparable (13.3 and 14.2 mmol plant—1 d—1, respectively, in the presence of DIC, and 14.7 and 14.0 mmol plant—1 d—1, respectively, in the absence of DIC), despite such a large difference in their concentrations in the nutrient feed solution. A higher proportion of NH4+ (up to 50 % of total N) had no further significant effect upon early vegetative growth, but in a long‐term experiment resulted in a high incidence of blossom end‐rot (BER) disease, thereby severely curtailing fruit yield. The presence of even 1.1 mM NH4+ reduced Ca2+ and Mg2+ accumulation in the leaves as well as in fruits.  相似文献   
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