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61.
The characterization of herbal materials is a significant challenge to analytical chemists. Goldenseal (Hydrastis canadensis L.), which has been chosen for toxicity evaluation by NIEHS, is among the top 15 herbal supplements currently on the market and contains a complex mixture of indigenous components ranging from carbohydrates and amino acids to isoquinoline alkaloids. One key component of herbal supplement production is botanical authentication, which is also recommended prior to initiation of efficacy or toxicological studies. To evaluate material available to consumers, goldenseal root powder was obtained from three commercial suppliers and a strategy was developed for characterization and comparison that included Soxhlet extraction, HPLC, GC-MS, and LC-MS analyses. HPLC was used to determine the weight percentages of the goldenseal alkaloids berberine, hydrastine, and canadine in the various extract residues. Palmatine, an isoquinoline alkaloid native to Coptis spp. and other common goldenseal adulterants, was also quantitated using HPLC. GC-MS was used to identify non-alkaloid constituents in goldenseal root powder, whereas LC-MS was used to identify alkaloid components. After review of the characterization data, it was determined that alkaloid content was the best biomarker for goldenseal. A 20-min ambient extraction method for the determination of alkaloid content was also developed and used to analyze the commercial material. All three lots of purchased material contained goldenseal alkaloids hydrastinine, berberastine, tetrahydroberberastine, canadaline, berberine, hydrastine, and canadine. Material from a single supplier also contained palmatine, coptisine, and jatrorrhizine, thus indicating that the material was not pure goldenseal. Comparative data for three commercial sources of goldenseal root powder are presented.  相似文献   
62.
A simple method for the analysis of major wine volatiles and semivolatiles by stir bar sorptive extraction in combination with thermal desorption and gas chromatography-mass spectrometry (SBSE-TD-GC-MS) was developed. Significant experimental parameters such as extraction time, temperature, salt addition, pH, and thermal desorption parameters were optimized to provide a sensitive and robust analytical method. The method provided good repeatability (%RSD < 10%) for 38 major wine volatile compounds, including alcohols, acids, esters, phenols, aldehydes, ketones, and lactones. Quantitative data for 62 South African red and white wines were used to study the suitability of major volatile data for the differentiation of wine samples according to grape variety or cultivar. Principal component analysis (PCA) and cluster analysis (CA) showed that most of the variation in volatile composition between wine samples could be ascribed to differences in wine age, wood contact, and fermentation practices. Despite the contribution of these factors, discriminant analysis (DA) was successfully applied to the classification of red and white wine samples according to cultivar.  相似文献   
63.
The quality of work and the output of a tractor-implement combination relies heavily upon the concentration and skill of the operator. Electronic systems are used increasingly to control tractor sub-systems, i.e. engine, transmission, implement hitch, external hydraulics, and driveline, and to monitor or control certain implements. However, current systems operate autonomously, relying entirely upon the operator for coordination. An integrated hierarchical control system could potentially monitor operating parameters pertinent to both the tractor and attached implements and use this information to control relevant tractor and implement sub-systems in a coordinated manner, thereby improving machine performance. Potential opportunities for the application of real-time, integrated, hierarchical control techniques to certain cultivation and crop establishment implements currently in use on European farms are reviewed. Specific implements (and their parameters) considered include primary cultivation machinery (control of working depth and working width), secondary cultivation machinery (control of working depth and seedbed quality) and crop establishment machinery (control of seeding depth and seed rate). Outline control strategies are proposed for these applications, and sensors and other hardware required to implement the control systems are identified. It is speculated that the agronomic and economic benefits which are likely to result from the implementation of the proposed technology, will enable economic justification of the proposed control systems in two to four operating seasons. These savings are likely to result from greater operational efficiency and more precise control of agronomic inputs.  相似文献   
64.
Eight herbicide combinations containing imazapyr alone or in mixture with glyphosate, triclopyr, or picloram+2,4-D were applied July–August 1991 at each of three upland sites in the southeastern U.S., with or without a subsequent broadcast burn in September. Seedlings of loblolly (Pinus taeda L.) or slash (P. elliottii Engelm. var. elliottii) pine were planted that winter. Second-year (August 1993) vegetation responses indicate that broadcast burning, in combination with herbicides, was associated with increased abundances of grasses, forbs, and shrubs and decreased abundances of vines and hardwood trees. In the presence of burning, imazapyr-in-mixture treatments were associated with a high abundance of grass (53% cover) and a mean size of pines less than that of seedlings growing in imazapyr-alone treatments. In the absence of burning, imazapyr-alone treatments were associated with a high abundance of blackberry (21% cover), an average height of hardwoods (131 cm) exceeding that of pines (71 cm), and a mean size of pines less than that of trees growing in imazapyr-in-mixture treatments. Hardwood height in the absence of burning was less when imazapyr was combined with glyphosate (75 cm) than with triclopyr (102 cm). Regardless of the presence or absence of burning, size of pines and abundance of shrubs and hardwoods did not differ between imazapyr rates of 0.56 and 0.84 kg acid equivalent/ha or among herbicides applied in mixture with imazapyr.  相似文献   
65.
Landscape Ecology - Whilst the composition and arrangement of habitats within landscape mosaics are known to be important determinants of biodiversity patterns, the influence of seascape patterning...  相似文献   
66.
Context

Lack of quantitative observations of extent, frequency, and severity of large historical fires constrains awareness of departure of contemporary conditions from those that demonstrated resistance and resilience to frequent fire and recurring drought.

Objectives

Compare historical and contemporary fire and forest conditions for a dry forest landscape with few barriers to fire spread.

Methods

Quantify differences in (1) historical (1700–1918) and contemporary (1985–2015) fire extent, fire rotation, and stand-replacing fire and (2) historical (1914–1924) and contemporary (2012) forest structure and composition. Data include 85,750-ha tree-ring reconstruction of fire frequency and extent; >?375,000-ha timber inventory following >?78,900-ha fires in 1918; and remotely-sensed maps of contemporary fire effects and forest conditions.

Results

Historically, fires?>?20,000 ha occurred every 9.5 years; fire rotation was 14.9 years; seven fires?>?40,469 ha occurred during extreme drought (PDSI <?? 4.0); and stand-replacing fire occurred primarily in lodgepole (Pinus contorta var. murrayana). In contemporary fires, only 5% of the ecoregion burned in 30 years, and stand-replacing fire occurred primarily in ponderosa (Pinus ponderosa) and mixed-conifer. Historically, density of conifers?>?15 cm dbh exceeded 120 trees/ha on?<?5% of the area compared to 95% currently.

Conclusions

Frequent, large, low-severity fires historically maintained open-canopy ponderosa and mixed-conifer forests in which large fire- and drought-tolerant trees were prevalent. Stand-replacing patches in ponderosa and mixed-conifer were rare, even in fires >?40,469 ha (minimum size of contemporary “megafires”) during extreme drought. In this frequent-fire landscape, mixed-severity fire historically influenced lodgepole and adjacent forests. Lack of large, frequent, low-severity fires degrades contemporary forest ecosystems.

  相似文献   
67.
68.
The cover image is based on Research Article Field evaluation of an unmanned aerial vehicle (UAV) sprayer: effect of spray volume on deposition and the control of pests and disease in wheat by Guobin Wang et al., DOI: 10.1002/ps.5321 . Illustration Credit: Guobin Wang, Yubin Lan, and Yuxing Han.

  相似文献   

69.
Potato psyllid, Bactericera cockerelli (?ulc), causes economic damage to potato crops throughout the major potato growing regions of western North America. When cultivated crops are not available, potato psyllid often occurs on non-crop hosts. In the southern U.S. and northern Mexico, native species of Lycium (Solanaceae) are important non-crop hosts for the psyllid. We determined whether Old World species of Lycium now widespread in the Pacific Northwest are reservoirs of potato psyllid in this growing region. We examined Lycium spp. across a wide geographic region in Washington, Oregon, and Idaho at irregular intervals during three growing seasons. Potato psyllids were present at all locations. To determine whether Lycium is also a host during intervals of the year in which the potato crop is not available, we monitored a subset of these sites over the entire year. Six sites were monitored at 1- to 3-week intervals from June 2014 to June 2016. Psyllids were present on Lycium throughout the year at all sites, including during winter, indicating that Lycium is also a host when the potato crop is seasonally not available. Psyllid populations included a mixture of Northwestern and Western haplotypes. We observed well-defined spring and fall peaks in adult numbers, with peaks separated by long intervals in which psyllid numbers were very low. Seasonal patterns in psyllid numbers on these non-native Lycium hosts were very similar to what has been observed on native Lycium in the desert southwest region of the U.S. Our findings demonstrate that potato psyllid associates with Lycium across a broad geographic region within the Pacific Northwest. These results will assist in predicting sources of potato psyllid colonizing potatoes in this important growing region.  相似文献   
70.
A 14-week-old male unilaterally cryptorchid Clumber spaniel was presented for acute lethargy. Physical examination revealed abdominal pain, and a single testis was palpated in the scrotum. Abdominal ultrasound and computed tomography (CT) revealed a poorly vascularized, ovoid structure immediately caudal to the left kidney with scant regional peritoneal effusion. Left intra-abdominal testicular torsion was confirmed at surgery, and routine cryptorchidectomy was performed. The patient recovered uneventfully from anesthesia and surgery.Key clinical message:The most common CT characteristics of testicular torsion were present in this case and correlated well with sonographic findings to allow for rapid, accurate diagnosis and surgical planning of unilateral, non-neoplastic, intra-abdominal cryptorchid testicular torsion in a juvenile dog. Contrast enhanced CT facilitated accurate localization of the undescended testis and evaluation of testicular perfusion and may be a useful alternative to ultrasound for diagnosing testicular torsion, especially in indeterminate cases.  相似文献   
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