Evaluation of crop water stress index for LEPA irrigated corn

This study was designed to evaluate the crop water stress index (CWSI) for low-energy precision application (LEPA) irrigated corn (Zea mays L.) grown on slowly-permeable Pullman clay loam soil (fine, mixed, Torrertic Paleustoll) during the 1992 growing season at Bushland, Tex. The effects of six different irrigation levels (100%, 80%, 60%, 40%, 20%, and 0% replenishment of soil water depleted from the 1.5-m soil profile depth) on corn yields and the resulting CWSI were investigated. Irrigations were applied in 25 mm increments to maintain the soil water in the 100% treatment within 60–80% of the “plant extractable soil water” using LEPA technology, which wets alternate furrows only. The 1992 growing season was slightly wetter than normal. Thus, irrigation water use was less than normal, but the corn dry matter and grain yield were still significantly increased by irrigation. The yield, water use, and water use efficiency of fully irrigated corn were 1.246 kg/m², 786 mm, and 1.34 kg/m³, respectively. CWSI was calculated from measurements of infrared canopy temperatures, ambient air temperatures, and vapor pressure deficit values for the six irrigation levels. A “non-water-stressed baseline” equation for corn was developed using the diurnal infrared canopy temperature measurements as T _c–T _a = 1.06–2.56 VPD, where T _c was the canopy temperature (°C), Ta was the air temperature (°C) and VPD was the vapor pressure deficit (kPa). Trends in CWSI values were consistent with the soil water contents induced by the deficit irrigations. Both the dry matter and grain yields decreased with increased soil water deficit. Minimal yield reductions were observed at a threshold CWSI value of 0.33 or less for corn. The CWSI was useful for evaluating crop water stress in corn and should be a valuable tool to assist irrigation decision making together with soil water measurements and/or evapotranspiration models. Received: 19 May 1998     

Tibial tuberosity advancement in 92 canine stifles: initial results,clinical outcome and owner evaluation

Objective To investigate the clinical outcomes, complications and owners' evaluation of the tibial tuberosity advancement (TTA) procedure in canine stifles. Methods A retrospective study of hospital records was performed to identify dogs diagnosed with partial or complete cranial cruciate ligament (CCL) rupture that had undergone TTA repair. Information obtained included signalment, period of lameness, surgical report, evidence of meniscal injury, postoperative recovery and peri-operative complications. Owners were asked to assess the long-term outcome. Results In a total of 72 dogs (median age, 6 years; median body weight, 34.8 kg), TTA was performed in 92 stifles. Twenty breeds were represented, with Labrador Retrievers and Rottweilers the most common. The period of lameness ranged from 3 days to 24 months. The median pre-operative lameness score was 3/4 and meniscal injury was present in 51 stifles. Minor complications occurred in 29% of cases. Major complications occurred in 6.5% of cases and consisted of meniscal injury and two tibial tuberosity fractures. All were successfully managed, with good limb function when subsequently assessed. In the owner evaluation, 96% reported moderate to great improvement postoperatively, with no lameness at rest and mild to no lameness after vigorous exercise. Conclusion Clinical outcome and owner evaluations in this case series indicate favourable results can be expected when CCL-deficient stifles are treated with TTA.     

Dose confirmation of moxidectin pour-on against natural nematode infections in lactating dairy cows.

The nematocidal effectiveness of moxidectin, administered topically at the rate of 500 mcg/kg BW, was determined for lactating dairy cows. Naturally infected animals were given either topical vehicle or moxidectin (Cydectin Pour-On Fort Dodge Animal Health) at the rate of 1 ml/10 kg BW (10 animals per treatment group), and sacrificed 14-18 days post-treatment for nematode enumeration. 100% efficacies were recorded for Ostertagia lyrata males, Cooperia punctata males and Oesophagostomum radiatum L4, with treatment group differences in geometric means significant (P < 0.05) for all. Populations of Trichostrongylus L4 and adult O. radiatum were also reduced by 100%, but low prevalence rates in the control animals precluded meaningful statistical inference. Nematode populations for which efficacies ranged from 96.7 to 99.6% (based on geometric means) and for which treatment group differences were significant (P < 0.05) included Ostertagia spp. adult females, inhibited L4 and developing L4, O. ostertagi adult males, Trichostrongylus axei adults and Cooperia spp. adult females. For all nematodes combined, moxidectin was 98.9% efficacious. In addition to exhibiting excellent nematocidal effectiveness, topical moxidectin was demonstrated to be safe, with animal health and milk production unaffected during the study.     

Reconstruction of a century of landscape modification and hydrologic change in a small urban watershed in Pittsburgh,PA

Assessing the causes of stream impairments is challenging without a clear understanding of the spatiotemporal interactions among human infrastructure networks and hydrologic systems. Landscape change is often characterized using simplistic metrics that lump changes into generalized categories, such as impervious cover. We examined the evolution of human infrastructure in Panther Hollow, a small watershed in Pittsburgh, Pennsylvania to characterize the impacts of long-term (~100 years) landscape change on stream flow. Results show that impervious cover in the catchment grew from 3 % in 1900 to 27 % in 2010. Growth was non-linear, with 60 % of the development occurring between 1904 and 1930. We then compared two models that predict changes in annual water yield, one model based on watershed impervious cover and one based on human infrastructure arrangement. The model based solely on impervious cover predicts excessive amounts of surface runoff relative to the infrastructure model and monitored yield. This discrepancy occurs because the impervious model does not account for the diversion of 50 % of the watershed drainage through the combined sewer system to an adjacent basin. In the Panther Hollow watershed, hydrology is dominated by a reduction in water yield, contrasting typical hydrologic changes associated with urbanization. Our analysis reveals the value of quantifying additional landscape metrics, such as infrastructure pattern and connectivity, which provide a more complete understanding of how human development alters natural hydrology.     

Salt transport organelle in Artemia salenis (brine shrimp)

The branchiae of Artemia adapted to triple-strength sea water (105 per mil salinity) were studied with the electron microscope. The epithelial lining of the metepipodite segment possesses organelles composed of stacks of disc-shaped mitochondria interlaced with flattened extensions of a canalicular system that in turn communicates with the plasma-bound surface of the cells. The distance between the canalicular and mitochondrial membranes is small and quite constant. The marked similarity to "mitochondrial pumps" in the anal papillae of tosquito larvae suggests that the organelle is concerned with salt transport.     

Inter‐specific hybridization between Black Sea trout (Salmo labrax Pallas, 1814) and rainbow trout (Oncorhynchus mykiss Walbaum, 1792)

Thermal shock‐induced triploid and unshocked control hybrids between rainbow trout (Oncorhynchus mykiss) and Black Sea trout (Salmo labrax) and their parental species were produced under hatchery condition by using heat shocks. Triploidization reduced eyed egg rate and alevin yield in all groups. Low survival rate was observed in both shock‐induced triploid hybrid and non‐shock‐induced control hybrids. Although hybrids demonstrated low body weight during the first feeding stage, they reached higher body weight by day 200 when compared with Black Sea trout and rainbow trout. A higher specific growth rate was calculated as 3.60 in the triploid hybrid groups, 1.41 in the triploid Black Sea trout groups and 2.27 in the triploid rainbow trout groups between days 110 and 200. A lower condition factor was determined in the hybrid than in the diploid parental species. A negative value of mid‐parent heterosis (MPH) performance was deected for condition factor, and a favourable MPH was detected for specific growth rate and weight in hybrids.     

Addition of Recombinant Human Growth Hormone to In Vitro Maturation Medium of Bovine Oocytes

The aim of this study was to increase the bovine embryonic development rate, adding recombinant human growth hormone (rhGH) to maturation medium of bovine oocytes. Oocytes were matured for 24 h in TCM 199 Earle's salts and five treatments were developed: T1, 0.01 IU/ml of recombinant human follicle stimulating hormone (rhFSH); T2, 0.01 IU/ml of rhFSH + 100 ng/ml of rhGH; T3, 0.01 IU/ml of rhFSH + 1000 ng/ml of rhGH; T4, 100 ng/ml of rhGH; and T5, 1000 ng/ml of rhGH at 39 degrees C and 5% of CO(2) in air and saturated humidity. In vitro fertilization from cumulus-oocyte complexes was conducted in TALP-Fert medium (18-22 h) and spermatozoa were selected by Percoll gradient. Zygotes were incubated in SOFaaci medium in 5% of CO(2) in air, 5% of O(2) at 39 degrees C and saturated humidity for 11 days. There was no statistical difference in cleavage rate and embryo production on day 7 and day 9 among treatments. However, the hatching rate increased significantly in the T4 and T5 treatments (11.0 and 12.8%, respectively), compared with the T1 treatment (4.6%) (p < 0.05). Therefore, the rhGH addition to the oocyte maturation medium showed beneficial effects on the hatching rate of in vitro-produced bovine embryos.