Soil Factors Affecting Heavy Metal Solubility in Some New Zealand Soils

Heavy metal contamination of soils is usually quantified and guidelines set solely on the basis of total heavy metal content. However, it is recognised that water soluble heavy metal concentrations may provide a better indication of the potential risk that heavy metals may pose to the soil environment. The aim of this study was to use a semi-empirical model based on the competitive adsorption of metal and H⁺ ions [dependent on solution pH, total metal content, total carbon content and soil oxide content] to predict water soluble Cu, Cr, Cd, Pb, Ni and Zn concentrations in a range of field contaminated soils. The results of multiple linear regressions showed that basic soil properties could predict 85, 72, 66, 78, 50 and 75% of the variation in soluble Ni, Cu, Cr, Pb, Cd and Zn concentrations respectively. Water soluble metal concentrations were best predicted using empirical linear regressions which included total metal content, while the importance of other soil properties such as soil pH, total carbon and oxalate extractable Fe and Al oxides varied between metals. The models have the potential to provide valuable information on metal availability in contaminated soils and offer an indication of the potential risk a metal may pose to a given soil environment, along with providing a basis for developing soil quality guidelines for the prevention, investigation and clean-up of soil metal contamination.     

Evaluation of an indirect oscillometric blood pressure monitor in normotensive and hypotensive anesthetized dogs

Objective – To determine the accuracy and precision of an oscillometric noninvasive blood pressure device as a predictor of invasive direct blood pressure in healthy anesthetized hypotensive and normotensive dogs. Design – Prospective observational study. Setting – University teaching hospital. Animals – Eight crossbred adult dogs. Interventions – Anesthesia was induced with propofol and maintained with isoflurane. A catheter was placed in the dorsal pedal artery to record systolic, mean, and diastolic arterial blood pressures (aSAP, aMAP, and aDAP, respectively). The noninvasive blood pressure device cuff was placed around the contralateral front limb to record noninvasive systolic, mean, and diastolic blood pressure (nSAP, nMAP, and nDAP). Two states of blood pressure (BP) were studied: baseline state was established by keeping end‐tidal isoflurane concentration at 1.2±0.1%. The hypotensive state was achieved by maintaining the same isoflurane concentration while withdrawing approximately 40% of the animal's blood volume until aMAP was stable at approximately 40 mm Hg. At the end of the study, blood was returned to the animal and it was allowed to recover from anesthesia. Measurements and Main Results – Agreement between the direct and indirect BP measurements was determined by the Bland‐Altman method. The SAP and MAP but not DAP bias varied significantly between each BP state. Normotensive absolute biases (mean [SD]) for SAP, MAP, and DAP were ?14.7 mm Hg (15.5 mm Hg), ?16.4 mm Hg (12.1 mm Hg), and ?14.1 mm Hg (15.8 mm Hg), respectively. Absolute biases during the hypotensive state for SAP, MAP, and DAP were ?32 mm Hg (22.6 mm Hg), ?24.2 mm Hg (19.5 mm Hg), and ?16.8 mm Hg (17.2 mm Hg), respectively. Conclusion – The oscillometric device was not reliably predictive of intra‐arterial BP during hypotension associated with acute hemorrhage.     

Intensive tree planting facilitates tropical forest biodiversity and biomass accumulation in Kibale National Park, Uganda

The extensive area of degraded tropical land and the calls to conserve forest biodiversity and sequester carbon to offset climate change demonstrate the need to restore forest in the tropics. Deforested land is sometimes replanted with fast-growing trees; however, the consequences of intensive replanting on biomass accumulation or plant and animal diversity are poorly understood. The purpose of this study was to determine how intensive replanting affected tropical forest regeneration and biomass accumulation over ten years. We studied reforested sites in Kibale National Park, Uganda, that were degraded in the 1970s and replanted with five native tree species in 1995. We identified and measured the size of planted versus naturally regenerating trees, and felled and weighed matched trees outside the park to calculate region-specific allometric equations for above-ground tree biomass. The role of shrubs and grasses in facilitating or hindering the establishment of trees was evaluated by correlating observed estimates of percent cover to tree biomass. We found 39 tree species naturally regenerating in the restored area in addition to the five originally planted species. Biomass was much higher for planted (15,675 kg/ha) than naturally regenerated trees (4560 kg/ha), but naturally regenerating tree regrowth was an important element of the landscape. The establishment of tree seedlings initially appeared to be facilitated by shrubs, primarily Acanthus pubescens and the invasive Lantana camara; however, both are expected to hinder tree recruitment in the long-term. Large and small-seeded tree species were found in the replanted area, indicating that bird and mammal dispersers contributed to natural forest restoration. These results demonstrate that intensive replanting can accelerate the natural accumulation of biomass and biodiversity and facilitate the restoration of tropical forest communities. However, the long-term financial costs and ecological benefits of planting and maintaining reforested areas need to be weighed against other potential restoration strategies.     

Studies in the Development of the Strawberry: III. Flower-Bud Initiation and Development in Large-Fruited Perpetual (“Remontant”) Strawberries

Summary

Four N application rates generated a range of tree N status as indicated by leaf N concentrations in six year old trees of `Mission' almond (Prunus dulcis (Mill.) D. A. Webb). Leaf N concentrations were related positively with concentrations of amino acids - especially asparagine and glutamine - in phloem and xylem saps. A pulse of 1.25 kg N per tree was applied to trees previously receiving 0, 78, 157 or 313 g N per tree, annually. Apparent uptake of soil N into above-ground tree parts was determined over a 39 d period. N uptake was determined as the difference in the products of tree biomass and tissue N concentrations before and after the N pulse. Tissue N concentrations increased signi®cantly in trees previously receiving the two lowest N application rates, whereas trees previously receiving the high N treatments were unresponsive. Tree N contents (0 g N per year, and also 78 g N per year treatments) increased slightly in response to the N pulse, although statistical differences were not observed due possibly to high variability in biomass among trees. At the same time, the amino acid concentrations in phloem exudate and xylem sap were highest in trees grown under the highest N fertilization regime. We suggest that the high amino acid concentrations in the phloem and xylem saps are indicative of a larger pool of amino N cycling throughout the vasculature of high N-status trees. Our data indicate an inverse relationship between tree N status, amino acid concentrations in xylem and phloem saps and capacity for soil N uptake in ®eld-grown trees. These results represent the ®rst ®eld data to correlate the concentration of amino acids circulating in the plant vasculature with soil N uptake. A functional relationship between the amino acid concentrations cycling within the tree and the regulation of soil N uptake has, however, not yet been established.     

Surgical treatment of perforating duodenal ulcer in a dog by use of modified choledochoduodenostomy and gastrojejunostomy

Modified choledochoduodenostomy and gastrojejunostomy were used successfully to treat extensive duodenal ulceration and perforation in a dog. Concomitant pyometra, simultaneously managed by ovariohysterectomy, may have predisposed the dog to stress ulceration. Choledochoduodenostomy was performed by transplantation of the entire common bile duct and associated major duodenal papilla to a more distal duodenal location. An indwelling choledochostomy tube was used to stent the anastomotic site, externally divert bile flow, and allow postoperative cholangiography. Early postoperative complications included presumed metronidazole-induced neurotoxicosis, choledochostomy tube obstruction, and pancreatitis, all of which resolved within 2 weeks. The dog was clinically normal and medication (ranitidine and sucralfate) was discontinued 35 months later.     

家禽营养中的烟酸

烟酸 ,又称尼克酸 ,是家禽营养中一个经常被遗忘的维生素。随着禽类遗传潜力的提高 ,有必要根据种类、生产性能和日粮基础成分来重新评估烟酸在日粮中的添加水平。烟酸是氧化还原反应的辅助因子 ,生长家禽缺乏烟酸会使生产性能降低、皮肤损伤、腿骨缩短和跗关节肿大 ,成年蛋鸡的产蛋量和孵化率降低。存在于谷物饲料中的烟酸大多是不能利用的 ,但有关烟酸生物学利用率的数据有限 ,需要更多方面的资料。肉鸡获得最佳生产性能所需烟酸添加量的估计值变化范围很广。但最新的研究结果显示 ,饲喂以小麦为基础日粮的肉鸡 ,烟酸的添加水平最高可达 1…     

Evaluating the utility of Arabidopsis thaliana as a model for understanding heterosis in hybrid crops

Despite the phenomenon of heterosis having been used to improve crop productivity for almost a century, there is little understanding of the molecular mechanisms involved. Heterosis has been described recently in the widely used plant model Arabidopsis thaliana. To assess the opportunity afforded by this system, we have developed, through pollination to a male sterile female parent (Ler ms1), a range of hybrids of A. thaliana accessions. We tested this method of hybrid production in A. thaliana by comparing seed produced by natural pollination and hand pollination of emasculated plants. Our results demonstrate that using male sterile lines, which more closely represent methods used for hybrid crop production, circumvents the problems associated with the analysis of hybrids produced from emasculated plants. We identified hybrid combinations exhibiting mid-parent heterosis for vegetative fresh weight ranging from −9 to 103% and characterised heterosis in the reciprocal hybrids of one strongly heterotic combination. We found no evidence of a relationship between the extent of genetic relatedness of A. thaliana accessions and the strength of heterosis exhibited by their hybrids. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.