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81.
Analysis of fenbendazole residues in bovine milk by ELISA 总被引:1,自引:0,他引:1
Brandon DL Bates AH Binder RG Montague WC Whitehand LC Barker SA 《Journal of agricultural and food chemistry》2002,50(21):5791-5796
Fenbendazole residues in bovine milk were analyzed by ELISAs using two monoclonal antibodies. One monoclonal antibody (MAb 587) bound the major benzimidazole anthelmintic drugs, including fenbendazole, oxfendazole, and fenbendazole sulfone. The other (MAb 591) was more specific for fenbendazole, with 13% cross-reactivity with the sulfone and no significant binding to the sulfoxide metabolite. The limit of detection of the ELISA method in the milk matrix was 7 ppb for MAb 587 and 3 ppb for MAb 591. Fenbendazole was administered in feed, drench, and paste form to three groups of dairy cattle. Milk was collected immediately before dosing and then every 12 h for 5 days. The ELISA indicated that residue levels varied widely among individual cows in each group. Fenbendazole levels peaked at approximately 12-24 h and declined rapidly thereafter. Metabolites were detected at much higher levels than the parent compound, peaked at approximately 24-36 h, and declined gradually. Residue levels were undetectable by 72 h. The ELISA data correlated well with the total residues determined by chromatographic analysis, but the use of the two separate ELISAs did not afford an advantage over ELISA with the single, broadly reactive MAb 587. The ELISA method could be used to flag high-residue samples in on-site monitoring of fenbendazole in milk and is a potential tool for studying drug pharmacokinetics. 相似文献
82.
S.R. Isberg P.C. Thomson F.W. Nicholas S.G. Barker & C. Moran 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2005,122(6):370-377
Crocodile morphometric (head, snout‐vent and total length) measurements were recorded at three stages during the production chain: hatching, inventory [average age (±SE) is 265.1 ± 0.4 days] and slaughter (average age is 1037.8 ± 0.4 days). Crocodile skins are used for the manufacture of exclusive leather products, with the most common‐sized skin sold having 35–45 cm in belly width. One of the breeding objectives for inclusion into a multitrait genetic improvement programme for saltwater crocodiles is the time taken for a juvenile to reach this size or age at slaughter. A multivariate restricted maximum likelihood analysis provided (co)variance components for estimating the first published genetic parameter estimates for these traits. Heritability (±SE) estimates for the traits hatchling snout‐vent length, inventory head length and age at slaughter were 0.60 (0.15), 0.59 (0.12) and 0.40 (0.10) respectively. There were strong negative genetic (?0.81 ± 0.08) and phenotypic (?0.82 ± 0.02) correlations between age at slaughter and inventory head length. 相似文献
83.
84.
The uncalibrated predictive ability of four preferential flow models (CRACK‐NP, MACRO/MACRO_DB, PLM, SWAT) has been evaluated against point rates of drainflow and associated concentrations of isoproturon from a highly structured and heterogeneous clay soil in the south of England. Data were available for four plots for a number of storm events in each of three successive growing seasons. The mechanistic models CRACK‐NP and MACRO generally gave reasonable estimates of drainflow over the three seasons, but under‐estimated concentrations of isoproturon over a prolonged period in the first season and over‐estimated them in the two remaining seasons. CRACK‐NP simulated maximum concentrations of isoproturon over the first two events of each of the three seasons of 156, 527 and 24.4 µg litre?1, respectively, and matched the observed data (465, 65.1 and 0.65 µg litre?1) slightly better than MACRO (69.1, 566 and 58.5 µg litre?1). Automatic selection of parameters from soils information within MACRO_DB reduced the emphasis on preferential flow relative to the stand‐alone version of MACRO. This gave a poor simulation of isoproturon breakthrough and simulated maximum concentrations were 0, 50.1 and 35.1 µg litre?1, respectively. The capacity model PLM gave the best overall simulation of total drainflow for the first two events in each season, but over‐estimated concentrations of isoproturon (967, 808 and 51.3 µg litre?1). The simple model SWAT represented total drainflow reasonably well and gave the best simulation of maximum isoproturon concentrations (140, 80.2 and 8.2 µg litre?1). There was no clear advantage here in using the mechanistic models rather than the simpler models. None of the models tested was able to simulate consistently the data set, and uncalibrated modelling cannot be recommended for such artificially drained heavy clay soils. © 2001 Society of Chemical Industry 相似文献
85.
86.
Epilepsy in the dog--a comparative approach 总被引:1,自引:0,他引:1
J Barker 《The Journal of small animal practice》1973,14(5):281-289
A review of the current theories of the physiopathogenesis of the epilepsies is presented; emphasis is placed on the value of a more basic approach to the naturally–occuring seizure disorders of the dog, from both a clinical and electroencephalographic viewpoint. The comparative and veterinary rewards of such an approach are stressed. Résumé. On a présenté une revue des théories courantes de la physiopathogenèse des épilepsies. On a fait ressortir la valeur d'une approche plus fondamentale concernant les troubles d'attaque brusque se produisant naturellement chez le chien d'un point de vue à la fois clinique et électro-encephalographique. On a souligné les récompenses comparatives et vétérinaires d'une telle approche. Zusammenfassung. Eine nochmalige Obersicht der zur Zeit herrschenden Théorie von der Physiopathogenèse ùber Epilepsien wird vorgelegt; nachdrùcklich betont man den Wert von einer mehr fundamentalen Stellungsnahme zu den spontanen Epilepsieanfall-Stǒrungen des Hundes, von klinischer wie elektroenzephalographischer Hinsicht aus. Man betont die relativen und veterinǎren Belohnungen von solch einer Annàherung des Problems. 相似文献
87.
Spontaneous gastric squamous cell carcinomas and other neoplasms in Greenland collared lemmings (Dicrostonyx groenlandicus). 下载免费PDF全文
Malignant neoplasms were present in 39/66 Dicrostonyx groenlandicus of varying ages, examined from a laboratory colony. The presence of multiple neoplasms in some resulted in an overall average of 1.15 tumors/affected animal. Gastric squamous papillomas were present in nine, and locally invasive or metastatic gastric squamous cell carcinomas in a further 36 animals. Three mammary adenocarcinomas, one pancreatic islet cell tumor, one probable pancreatic adenocarcinoma and one adrenal cortical adenoma were also seen in lemmings with gastric tumors. Two others had mammary adenocarcinoma alone, and one animal had bilateral Harderian gland adenocarcinoma. Lesions resembling glomerulonephrosis of rats were seen in 23/51 animals whose kidneys were examined. These findings were not considered artefacts of captivity since concurrent gastric squamous cell carcinoma, mammary adenocarcinoma and glomerulonephrosis were present in the single animal examined directly from the wild at Eskimo Point, Northwest Territories. 相似文献
88.
A device is described which can produce single drops of liquids in uniform sizes. Drop size can be adjusted, and with an aqueous solution of a non-ionic surfactant (“Agral”; 1 ml/litre) sizes 160–490 μm diam. were produced. Suitable techniques of calibration are given. The device is a useful research tool for study of the effects of form of deposit on herbicide performance. 相似文献
89.
Colin E. Harvey 《The Journal of small animal practice》1977,18(3):163-168
Three dogs and one cat were diagnosed at the University of Pennsylvania Veterinary Hospital as having parotid salivary duct rupture and fistula as a result of previous surgery. Sialography confirmed the diagnosis. Two animals were treated by ligation, and two by anastomosis of the parotid duct. All four animals returned to normal after treatment. 相似文献
90.
In vitro antimicrobial sensitivity of Mycoplasmas isolated from the bovine genital tract. 总被引:1,自引:1,他引:0 下载免费PDF全文
R B Truscott O Onoviran H L Ruhnke N A Fish C A Barker 《Canadian journal of veterinary research》1975,39(4):416-420
The in vitro antimicrobial sensitivity of 14 mycoplasma and 13 ureaplasma strains isolated from the genital tracts of bulls was examined. It was found that at relatively low concentrations, tetracycline, declomycin and tylosin were lethal to both types of organisms. Lincospectin, berenil, streptomycin and erythromycin were lethal to mycoplasmas but were only inhibitory to the ureaplasma strains at the same concentrations. Polymyxin B and novobiocin were ineffective at the levels tested. 相似文献