排序方式: 共有86条查询结果,搜索用时 7 毫秒
81.
82.
Belovari T Stević N Gajović S Kostović-Knezević L 《Anatomia, histologia, embryologia》2004,33(2):90-95
Different experimental systems are used to study developmental processes in mammals. In this study, three experimental models were analysed and correlated: (1) cultivation of rat embryos in vitro; (2) cultivation in vitro and then transplantation in vivo; (3) direct transplantation in vivo. When embryos were cultivated in vitro and then transplanted in vivo, after the initial in vitro restriction, developmental potential was recovered. The in vitro restriction depended on medium used and duration of culture. Pre-cultivation in serum-free medium for 7 days restricted developmental potential for nervous tissue, and for 14 days restricted developmental potential for skeletal muscles, adipose tissue and glandular epithelia. Transferrin addition improved in vitro differentiation of neuroblasts, cartilage and columnar epithelium. In the combined in vitro and in vivo method, transferrin preserved developmental potential in comparable extent to the addition of the serum. Even in serum-free conditions in vitro, the subsequent in vivo wide expression of developmental potential was possible. Therefore, the combination of in vitro and in vivo methods turned to be advantageous than the isolated approaches (in vitro or in vivo only), and enabled testing in more detail the influence of a single substance on developmental course and potential. 相似文献
83.
Sadif Dunya Tatjana Kistner Heiner Grüneberg Cornelia Oschmann Prof. Dr. Christian Ulrichs 《Gesunde Pflanzen》2005,57(8):193-198
Zusammenfassung Untersucht wurde, inwiefern sich Lactobacillus ssp. und Bacillus subtilis im Rahmen einer Gleisbettbegrünung positiv auf Wachstumsparameter und Bedeckungsgrad mit Sedum album auswirken. Die Versuche wurden auf den drei Substraten Ziegelbruch, Mineralwollmatten und Geotextilmatten im Jahre 2003 an der Humboldt-Universität zu Berlin durchgeführt. Zusätzlich wurden alle Varianten mit und ohne Nährmedium getestet.Aus den Versuchsergebnissen geht hervor, dass die Substratbeschaffenheiten und die induzierten Bakterienkulturen sehr stark das Pflanzenwachstum beeinflussen. Lediglich Ziegelbruchsubstrat zeigte gute Voraussetzungen für die Entwicklung des aeroben Bakteriums B. subtilis. Dieses ist physikalisch relativ stabil gegenüber Umwelteinflüssen und durch seine grobe Struktur ist auch eine gute Durchlüftung gewährleistet.Die Zugabe eines Nährmediums hatte in allen Versuchen auch ohne Applikation von Bakterien einen entscheidenden Einfluss auf die Wachstumsparameter als auch auf einen frühzeitig hohen Bedeckungsgrad. Drei Monate nach dem Versuchsbeginn konnten keine signifikanten Unterschiede zwischen den Behandlungen mehr nachgewiesen werden. Der Bedeckungsgrad lag zu diesem Zeitpunkt bereits bei mindestens 97%. Der Einsatz der beiden Bodenbakterien und/oder des Nährmediums hatten innerhalb der ersten zwei Monate eine gegenüber der Kontrolle schnellere Bedeckung des Substrats, basierend auf einem verstärkten Wachstum, zur Folge. 相似文献
84.
Tamara Lazarević-Pašti Tatjana Momić Miloš M. Radojević Vesna Vasić 《Pesticide biochemistry and physiology》2013
Inhibitory effects of five organophosphorus pesticides (diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate) and their oxo-analogs on human myeloperoxidase (MPO) activity were investigated. While inspecting separately peroxidase and chlorination activity, it was observed that investigated OPs affect peroxidase activity, but not chlorination activity. Among investigated pesticides, malathion and malaoxon have showed the highest power to inhibit MPO peroxidase activity with IC50 values of the order of 3 × 10−7 and 5 × 10−9 M, respectively. It was proposed that inhibition trend is rendered by molecular structure which invokes steric hindrance for OPs interaction with MPO active center responsible for peroxidase activity. In addition, it was concluded that physiological function of MPO is not affected by any of the investigated OPs. 相似文献
85.
Silvija Cerni Dijana Skoric Jelena Ruscic Mladen Krajacic Tatjana Papic Khaled Djelouah Gustavo Nolasco 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(4):701-706
Citrus tristeza virus (CTV) represents one of the major threats to citrus production worldwide. In the East Adriatic region, CTV symptoms are mostly
absent due to traditional citrus grafting on trifoliate orange (Poncirus trifoliata), a CTV-tolerant rootstock. Therefore, the virus has been continuously spreading by the propagation of infected material.
The genetic variability of CTV was studied on nineteen citrus samples, collected from orchards in the coastal region of Croatia,
Montenegro and Albania, that previously tested positive by ELISA and immunocapture RT-PCR. Single-strand conformation polymorphism
of the amplified coat protein gene demonstrated the presence of different CTV variants in each amplicon, while sequence analysis
of cloned CP gene variants confirmed their clustering into six out of the seven phylogenetic groups so far delineated. Four
of these groups include sequences of severe quick decline, seedling yellows and stem-pitting (SP) isolates, thought to be
found only rarely in the Mediterranean region. Regardless of the lack of symptoms in the field, CTV isolates from the East
Adriatic displayed high genetic variability and pathogenic potential, additionally confirmed by biological characterisation.
The high percentage of mixed infections suggest the potential for further diversification and a greater risk of severe variants
spreading into new areas. 相似文献
86.
Canine babesiosis, caused by intraerythrocytic Babesia spp., is a tick-borne disease of worldwide importance. No information on canine babesiosis has been documented in Slovenia. Therefore, 238 dogs admitted to the Small animal clinic in Ljubljana from the years 2000 to 2002 were tested for the presence of babesial parasites in the blood. Based on clinical, microscopic and molecular investigations, 14 dogs (5.9%) were determined as being infected with babesiae. Clinical signs relating to acute haemolysis, fever, anorexia, depression and haematological abnormalities such as anaemia and thrombocytopenia were noticed in most of the 14 infected dogs. The morphology of the parasites was indicative of Babesia canis infection. Two subspecies were detected, namely B. canis canis (11 dogs, 4.6%) and B. canis vogeli (3 dogs, 1.3%) using PCR and subsequent sequence analysis of portions of nns rRNA gene. In addition, based on nucleotide sequence analysis, the 11 isolates of B. c. canis could be subdivided into three groups, whereas the three B. c. vogeli isolates were genetically identical. The results of this study demonstrate the presence of canine babesiosis due to B. c. canis and B. c. vogeli in Slovenia. 相似文献