Ghrelin在绵羊卵母细胞体外成熟过程中对BCL-2、BAX的mRNA表达的影响

为了探讨Ghrelin在绵羊卵母细胞体外成熟过程中与BCL-2、BAX的相关性及Ghrelin在绵羊卵母细胞成熟过程中可能的作用机理,试验在绵羊卵母细胞成熟液中添加500 ng/mL Ghrelin,采用实时荧光定量PCR分别在0,8,16,24小时时检测卵母细胞BCL-2、BAX的表达量。结果表明:试验组添加Ghrelin后8,16小时时卵母细胞BCL-2 mRNA相对表达量显著升高(P<0.05),但成熟后16,24小时时较8小时时有下降趋势;下降到24小时时,其表达量与0小时时没有显著变化(P>0.05);8,16小时时试验组与对照组比较差异显著(P<0.05),0,24小时时差异不显著(P>0.05)。试验组添加Ghrelin后8,16,24小时卵母细胞BAX mRNA相对表达量较0小时时下降,且差异显著(P<0.05),但成熟后16,24小时时与8小时时比较有上升趋势;在8,16小时时试验组较对照组BAX mRNA相对表达量均有所下降,且差异有显著性(P<0.05),而在0,24小时时差异不显著(P>0.05)。说明Ghrelin在绵羊卵母细胞成熟过程中与BCL-2和BAX的表达存在相关性,推测Ghrelin在卵母细胞成熟过程中存在积极调控作用。     

Campylobacter jejuni is associated with, but not sufficient to cause vibrionic hepatitis in chickens

Vibrionic hepatitis is a disease of poultry which is characterised by the presence of focal lesions in the liver, usually 1-2mm in size and greyish-white in colour. The cause of the disease remains unclear, as do the reasons for its recent re-emergence. We examined the livers of commercial broiler chickens taken during processing and found Campylobacter spp. in both normal livers and those displaying signs indicative of focal hepatitis. Livers with signs of hepatitis had significantly more Campylobacter spp. present than those without and other bacterial genera were infrequently present. We were unable to replicate the disease in a healthy host following experimental infection with a Campylobacter jejuni strain isolated from a liver showing signs of focal hepatitis. However, a significant T cell response to C. jejuni was seen in the liver of Campylobacter infected birds. We conclude that the presence of Campylobacter spp. in the liver alone is not sufficient to cause vibrionic hepatitis, but that a predisposing factor, possibly within the host is required. We also provide evidence that chickens mount an adaptive T cell response to systemic C. jejuni.     

Leucoencephalomyelopathy in Australian Rottweiler dogs

Clinical and pathological studies of a neurological disorder of 3 adult Rottweiler dogs are described. Dogs developed proprioceptive losses involving the limbs, leading to progressively worsening ataxia and hypermetria. Blood and CSF fluid analyses were normal and radiographs and myelograms showed no compressive lesions of the spinal cord. At necropsy, severe demyelination of the dorsal and lateral funiculi of the cervical spinal cord was visible grossly. Microscopically there was more extensive, symmetrical involvement of the spinal cord and brain than was apparent grossly, with demyelination of white matter tracts and sparing of axons in affected areas. These changes were considered diagnostic for leucoencephalomyelopathy. This condition has not been previously reported in Australia, and is probably transmitted genetically, presumably as an autosomal recessive trait.     

内蒙古镶黄旗草原生态环境退化原因及可持续发展

以内蒙古自治区草原生态环境严重恶化、经济落后的镶黄旗为研究对象，通过对制约镶黄旗草原生态系统可持续发展的因素进行分析，并结合镶黄旗实际情况，提出适合该区域草原生态环境及经济可持续发展的思路和对策。     

西南矮马矮小性状基因SHOX的克隆测序和多态性研究

本研究首次克隆出西南马矮小性状基因完整第二外显子序列,GenBank登录号为EU556739;通过PCR-SSCP方法对8个品种150匹马的该序列做了多态性分析,在P2位点上268处有1个G→A的突变,和431处有1个T→G的突变导致了等位基因B变为等位基因A.该位点除云南文山马(WS)的优势基因型和优势等位基因分别为AB型和B外,其余类群马均为BB型和B.云南丘北马(QB)、云南文山马(WS)、云南大理马(DL)、建昌马(JC)和设特兰马(ST)在P2位点上处于Hardy-Weinberg平衡状态.     

土霉素药渣栽培杏鲍菇可行性研究

以杏鲍菇为试材,在玉米芯中添加不同比例的土霉素药渣作为栽培杏鲍菇的辅料,对土霉素药渣栽培杏鲍菇的可行性进行研究和分析。结果表明:土霉素药渣含有木质素15.58%、纤维素39.62%、蛋白质50.02%及灰分13.15%;玉米芯中随着土霉素药渣添加比例的增加,呈现菌丝生长速率加快、满瓶天数减少的趋势,且添加适宜比例的土霉素药渣,可显著提高杏鲍菇鲜菇产量和生物学效率,其中83%玉米芯添加15%土霉素药渣的处理较优;杏鲍菇对土霉素的降解率可达到99.9%,4个添加土霉素药渣处理的杏鲍菇子实体均未检测到土霉素残留。     

几种生态因子对红砂和霸王种子萌发与幼苗生长的影响

自然条件下进行了土壤温度、水分、播深及覆沙地境对红砂和霸王种子萌发与幼苗生长的效应研究,结果显示,模拟阵雨期(人工洒水)覆沙和未覆沙试验小区白天播种层土壤温度均较高,尤其中午(14:00)覆沙小区1和4 cm播种层土壤温度平均值分别高达43.2和37.3℃,未覆沙小区分别高达38.0和33.6℃.高温引发种子次生休眠是该期红砂和霸王种子不能萌发的主要原因.而且播种层土壤含水量日内下降十分明显,其中覆沙和未覆沙小区1 cm土层平均土壤含水量下降至0.11%和2.62%,也会引发种子产生次生休眠,成为红砂和霸王种子不能萌发的另一原因.自然连续降水期2种试验小区播种层平均土壤温度适宜,播种层土壤含水量高而稳定,满足了红砂和霸王种子萌发的适宜温度和较高水势的条件.因此,在干旱荒漠区进行荒漠草地的人工辅助建植,需要依靠连续性降水过程.自然条件下霸王发芽率最高的播深处理在覆沙小区为2 cm、未覆沙小区为1 cm;在2种试验小区内霸王幼苗生物量最大的播深处理均为2和3 cm.覆沙较未覆沙地境有利于霸王种子萌发和幼苗的生长.播种当年霸王地上生物量为红砂的75～117倍.     

松材线虫对黑松几个生理生化指标的影响

8～9年生黑松经4种处理(接种松材线虫、100μg苯甲酸水溶液处理、无菌水处理并环剥主干中部树皮以及单独无菌水处理)后,研究了其流脂量、蒸腾作用及叶部过氧化物酶同工酶的变化.结果显示,接种后12天,所有接虫株松脂分泌停止;接种后27天,接虫株蒸腾作用下降最明显,其平均蒸腾强度为0.0145ml·g