Improved solution for vitrification of Prochilodus lineatus embryos based on the reduction in risk factors: Toxicity,osmotic responses and ice‐nucleation

The objective of our work was to describe a low toxicity cryoprotectant solution that allowed vitreous solid formation. Embryos of Prochilodus lineatus were submitted to sensitivity evaluations of six internal cryoprotectants (dimethyl sulphoxide – Me₂SO, dimethyl acetamide – DMA, dimethyl formamide – DMF, methanol – MET, glycerol – GLY and 1,2‐propanediol – PROP) at concentrations of 1–6 M; and two external cryoprotectants (sucrose – SUC and glucose – GLU) at concentrations of 0.1–1 M for 20 min. The capacity of the cryoprotectant solutions to exchange heat with the medium and to form glassy solids was evaluated by immersing 10 μl of cryoprotectant in liquid nitrogen. The PROP had a high survival rate at all concentrations evaluated, and was the only substance that allowed a vitreous solid formation. Thus, it is concluded that the PROP‐6 M was the most adequate solution for embryonic vitrification processes, because heat exchange between the system (PROP 6 M/embryos/liquid nitrogen) was faster than for other cryoprotectants and combinations thereof; has low toxicity, promote high rates of dehydration in short periods, and reach the vitreous state, being a good candidate to be used in the tests of embryonic vitrification.     

A new cytotoxic naphthopyrone dimer from Paepalanthus bromelioides.

A new naphthopyrone dimer was isolated from the capitula of Paepalanthus bromelioides by chromatographic procedures. It structure was deduced from spectrometric data. On colorimetric assay for cytotoxicity the new dimer showed IC50 of 55.9 microM.     

Identification of bacterial protein markers and enolase as a plant response protein in the infection of Olea europaea subsp. europaea by Pseudomonas savastanoi pv. savastanoi

Olive knot disease is characterised by the development of galls on Olea europaea stems as a result of infection by Pseudomonas savastanoi pv. savastanoi. Protein differential accumulation during the first week of infection was studied using two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry to investigate the biochemical changes occurring in infected tissues and to understand the factors involved in bacteria pathogenesis and plant response to infection. Common infection symptoms were obtained in 1 year-old plants of two Portuguese cultivars, ‘Galega’ and ‘Cordovil de Serpa’ using the strain NCPPB 2327. The comparison of protein patterns of non-inoculated stem tissues, stem tissues inoculated with water or with the strain NCPPB 2327 led to the detection of differentially expressed infection-related proteins. Moreover a distinct protein pattern was obtained between cultivars in response to infection. The differential protein expression was characterised by qualitative and quantitative variation. Among the differentially expressed proteins were the bacterial P. savastanoi orthologues of outer membrane porin F, the tellurium resistance protein, aconitate hydratase 2 and a hypothetical protein with unknown function. From O. europaea, protein orthologues of enolase and calcium-dependent protein kinase were found to be differentially expressed. Results are discussed in the context of the molecular basis of plant–pathogen interactions in the search for markers for the presence of the bacterium in plant tissues.     

C, N, and noble gas isotopes in grain size separates of presolar diamonds from efremovka

Nanometer-size presolar diamonds from the Efremovka CV3 chondrite were physically separated into several grain size fractions by ultracentrifugation. The coarsest size fraction is the most enriched in carbon-12; the others have broadly similar carbon isotopic compositions. Measurement of noble gases shows that their concentration decreases with decreasing grain size. This effect is attributed to ion implantation. Such an episode could occur in the envelope of a supernova that produced the diamonds, or in interstellar space; in either case, ions with energies above a certain threshold pass completely through the smaller diamond grains without being captured. Concentrations of nitrogen show only minor variations with grain size, indicating a different mechanism of incorporation into the diamonds.     

Loss of intraspecific aggression in the success of a widespread invasive social insect

Despite the innumerable ecological problems and large economic costs associated with biological invasions, the proximate causes of invasion success are often poorly understood. Here, evidence is provided that reduced intraspecific aggression and the concomitant abandonment of territorial behavior unique to introduced populations of the Argentine ant contribute to the elevated population densities directly responsible for its widespread success as an invader. In the laboratory, nonaggressive pairs of colonies experienced lower mortality and greater foraging activity relative to aggressive pairs. These differences translated into higher rates of resource retrieval, greater brood production, and larger worker populations.     

Heme oxygenase-1 activity is involved in the control of Toxoplasma gondii infection in the lung of BALB/c and C57BL/6 and in the small intestine of C57BL/6 mice

Heme oxygenase-1 (HO-1) is an enzyme that catabolizes free heme, which induces an intense inflammatory response. The expression of HO-1 is induced by different stimuli, triggering an anti-inflammatory response during biological stress. It was previously verified that HO-1 is able to induce indoleamine 2,3-dioxygenase (IDO), an enzyme that is induced by IFN-γ in Toxoplasma gondii infection. To verify the role of HO-1 during in vivo T. gondii infection, BALB/c and C57BL/6 mice were infected with the ME49 strain and treated with zinc protoporphyrin IX (ZnPPIX) or hemin, which inhibit or induce HO-1 activity, respectively. The results show that T. gondii infection induced high levels of HO-1 expression in the lung of BALB/c and C57BL6 mice. The animals treated with ZnPPIX presented higher parasitism in the lungs of both lineages of mice, whereas hemin treatment decreased the parasite replication in this organ and in the small intestine of infected C57BL/6 mice. Furthermore, C57BL/6 mice infected with T. gondii and treated with hemin showed higher levels of IDO expression in the lungs and small intestine than uninfected mice. In conclusion, our data suggest that HO-1 activity is involved in the control of T. gondii in the lungs of both mouse lineages, whereas the hemin, a HO-1 inducer, seems to be involved in the control of parasitism in the small intestine of C57BL/6 mice.     

Short-Term Effects of Duodenocecostomy on Body Weight,Glucose Absorption,Serum Components,and Intestinal Histopathology in Four Normal Horses

The objective of this study was to evaluate duodenocecostomy in horses performed through a ventral midline laparotomy and report its influence on body weight, glucose absorption, serum components, and characteristics of jejunum, cecum, and large colon histology. Four horses were submitted to the duodenocecostomy technique through a ventral midline laparotomy with animals in dorsal recumbency under inhalation anesthesia, followed by abdominal exploration. A side-to-side anastomosis was performed between the duodenojejunal flexure and the base of the cecum with two simple continuous suture lines of the serosal and muscular layers. The size of the opening created was approximately 2 cm in diameter. The mucosa layer was not sutured. After 30 days, animals were submitted to a second laparotomy to check the patency of the duodenocaecal fistula. During both laparotomy procedures, excisional biopsies of different segments of the gastrointestinal tract were performed. Information on physical examination findings, results of hematologic and histopathologic evaluations, and oral glucose absorption test were recorded. The horses did not have significant weight loss from baseline, and absorption curve of glucose did not significantly vary from baseline. Only triglycerides had significant alterations. Histologic evaluation of jejunum, cecum, and large colon did not show alterations of intestinal structure and morphology. We concluded that the proposed technique, principally in relation to the fistula size and not suturing the mucosa layer, allowed partial or total occlusion of the fistulae without the necessity of a second surgery and avoided the permanent bypass of ingesta and weight loss.     

Soil erosion and hydrological response to land abandonment in a central inland area of Portugal

This paper analyses the effects of land abandonment on runoff and sediment yield in a central inland area of Portugal. Rainfall simulation experiments were performed in areas of traditional land use (land cultivated with cereals) with various types of soil plant cover associated with the period of abandonment (4–5 years, 15–20 years and 30–40 years) in two different seasons, namely under very dry conditions and after a long wet period. Statistical analysis showed a notable difference between the cultivated soils and those abandoned for a long period of time, which included appreciable vegetation recovery, higher organic matter content and negligible overland flow and soil loss. Cereal cultivation, in particular ploughing, is a very negative land management practice, due to the high runoff and soil loss. Within 4–5 years a crust develops over abandoned soils with sparse herbaceous plant cover, particularly after a dry season, which enhances runoff and sediment yield. In scrubland and oak plots, although marked water repellency was registered at the end of a dry, warm season, its influence seems to be irrelevant in terms of overland flow and erosion yield. The major factor in controlling overland flow and reducing soil erosion rates appears to be the role played by vegetation in protecting the soil surface and supplying organic carbon to the top layer. Copyright © 2010 John Wiley & Sons, Ltd.