Steam-rolled wheat diets for finishing cattle: effects of dietary roughage and feed intake on finishing steer performance and ruminal metabolism

Two experiments were conducted to determine the influence of dietary roughage concentration and feed intake on finishing steer performance and ruminal metabolism. In Exp. 1, 126 steers (334 kg) were used in a completely randomized design and fed (120 d) diets of steam-rolled wheat without roughage or containing 5, 10 or 15% roughage (50% alfalfa hay:50% corn silage). Steers fed 5 or 10% roughage gained faster (quadratic, P less than .05) and were more efficient (quadratic, P less than .05) than steers fed 15% or no roughage. In Exp. 2, six ruminally cannulated steers (447 kg) were used in a 6 x 6 latin square design and fed (twice daily) diets of steam-rolled wheat without roughage or containing 5 or 15% alfalfa hay at twice or three times NE required for maintenance. Increasing dietary roughage increased (linear, P less than .01) ruminal liquid passage 38%, indigestible ADF passage 63%, Yb-labeled wheat passage 75% and fiber fill 31%. The rate of in situ starch digestion tended to increase (linear, P = .16), and ruminal VFA concentration was 40 mM higher (P less than .01) at 4 h after feeding with increased roughage. Increased feed intake increased (P less than .05) ruminal starch fill, fiber fill, liquid fill and liquid passage 23%, Yb-labeled wheat passage 50% and Dry-labeled hay passage 20%. It reduced protozoa five- to sixfold (P less than .01) but doubled total bacterial counts (P less than .01). Ruminal NH3N was lower (P less than .01) and total VFA concentration was 50 mM higher (P less than .01) at 4 h after feeding. The acetate:propionate ratio was reduced from 2.3 to 1.3 (P less than .01) with increased intake. Adding roughage to a steam-rolled wheat diet increased passage and tended to increase rate of starch digestion; increased feed intake with its associated effects on ruminal fill and passage dramatically shifted the microbial population and fermentation end products.     

Chronic cervical compressive myelopathy in horses: clinical correlations with spinal cord alterations

Histological examination was performed on the cervical spinal cord from 13 horses with chronic cervical compressive myelopathy of 4 to 29 months duration. Structural alterations were correlated with clinical features. At the level of compression, the spinal cord was grossly deformed. Histological alterations included nerve fibre swelling and degeneration, occasional spheroids, astrocytic gliosis, increased macrophage activity and increased perivascular collagen. Myelin degeneration or loss at the level of the compressive lesion was greatest in the ventral and lateral funiculi and less consistently present in the dorsal funiculi. Asymmetry of lesions in the dorsal funiculi was associated with asymmetry of clinical signs in 5 horses. Histological alterations in areas of Wallerian degeneration were similar to that at the level of spinal cord compression, except that perivascular collagen was not increased. Wallerian degeneration was present cranial to the compressed site in the superficial portions of the lateral funiculi and in the middle of the dorsal funiculi. Caudal to the compressed site it was present in the ventral funiculi adjacent to the ventral median fissure and in the middle of the lateral funiculi. Deformation of the spinal cord did not correlate with the severity or duration of clinical signs but was positively correlated with the amount of perivascular collagen increase. The amount of nerve fibre swelling was not correlated with the severity of clinical signs but was negatively correlated with their duration. A rapid loss of nerve fibres apparently occurred early in the course of compression, since there was a marked decrease in the amount of nerve fibre swelling and Marchi stained degenerating myelin with increasing clinical duration.(ABSTRACT TRUNCATED AT 250 WORDS)     

Serum biochemical and electrophoretic values from four deer species and from pronghorn antelope.

Serums from 4 species of deer and 1 species of antelope were analyzed for various components in order to define an animal disease model for sickle cell disease in people. Animal species included black-tailed deer (Odocoileus hemionus columbianus), mule deer (Odocoileus hemionus), sika deer (Cervus nippon nippon), fallow deer (Dama dama), and pronghorn antelope (Antilocapra americana). The mean serum values for total bilirubin, total protein, albumin, creatinine, urea nitrogen, and electrolytes were similar in all species and were in the normal range for human beings. Cholesterol and uric acid values for all animals were lower than those for people. Alkaline phosphatase values in the 4 cervid species were higher than in the pronghorn antelope. Values for glutamic oxalacetic transaminase were lower in the cervids than in the pronghorn antelope. Lactic dehydrogenase values were similar in the 5 species. High activities for glutamic oxalacetic transaminase and lactic dehydrogenase in the 5 species probably related to muscle mass and great muscular activity.     

Echinoid spawning induced by a radial nerve factor

An extract of radial nerves of the sea urchin. Strongylocentrotus purpuratus, induces spawning within 1 minute in this species as well as in other echinoids, but a delayed spawning in several asteroid species. The concentration of the spawn-inducing factor in the radial nerves fluctuates annually correlated with the reproductive season of this species along the coast of southern California.     

An outbreak of avian malaria in captive yellowheads/mohua (Mohoua ochrocephala)

CASE HISTORY: Eight mohua, or yellowheads (Mohoua ochrocephala), were held in a large open aviary over the summer months of 2003–2004, following their capture for captivebreeding purposes. Two birds died of transportation trauma shortly after arrival, one became ill and died a month later, and another four died within a 2-week period in February 2004. The eighth bird also became ill at this time but survived for a year following treatment with chloroquine and doxycycline.

CLINICAL AND PATHOLOGICAL FINDINGS: The affected birds were depressed, lethargic and dyspnoeic. Necropsy of three birds showed a slightly pale and swollen liver and spleen. Impression smears of the liver of one bird revealed schizonts resembling Plasmodium spp. within the cytoplasm of many hepatocytes, which was confirmed histopathologically. Similar protozoal organisms were seen within splenic histiocytes and pulmonary endothelial cells of 5/6 birds. Electron microscopy identified these as protozoal schizonts containing merozoites of similar size and structure to those of Plasmodium spp.

DIAGNOSIS: The birds were infected with a protozoal haemoparasite resembling Plasmodium spp.; asexual stages within hepatocytes and endothelial cells of the lung and spleen were typical of this organism.

CONCLUSIONS AND CLINICAL RELEVANCE: The mohua captured from west Otago were highly susceptible to avian malaria as they came from an isolated population that was likely to be naïve and have had no previous contact with this organism. The birds were probably infected by bites from mosquitoes feeding off local populations of blackbirds subsequently found to be infected with Plasmodium spp.     

Failure of zinc supplementation to prevent severe facial eczema in cattle fed excess copper

CASE HISTORY: A 400-cow dairy unit in the Waikato suffered a severe outbreak of facial eczema (FE) despite consistent zinc supplementation and significantly elevated serum zinc concentrations.

CLINICAL FINDINGS: FE prevention had begun in mid-January 2006, via zinc sulphate supplementation in the water. Photosensitisation was reported on 06 April, contemporaneous with a marked increase in the number of Pithomyces chartarum spores in pasture samples. Within 10 days of this first clinical case, 100 affected cows had been dried off, eight of which subsequently died or were culled. Blood samples were collected from ten affected cows; all had serum zinc concentrations >17 µmol/L, and eight had gamma-glutamyl transferase (GGT) activities >200 U/ml, indicating that the photosensitisation was secondary to liver damage, i.e. probably FE. Further investigation identified that this herd had been receiving excess copper supplementation; 4/6 culled cows had liver copper concentrations within or above the marginal range for copper toxicity.

DIAGNOSIS: Severe FE despite zinc supplementation that increased serum zinc concentrations above recommended levels

CLINICAL RELEVANCE: As zinc supplementation significantly reduces apparent copper status, copper supplementation is often used to counteract this. Previous excess copper intake may reduce the efficacy of zinc in preventing FE, thus copper intake should be assessed prior to the start of zinc supplementation.     

Lesions in the brains of three cattle resembling the lesions of enterotoxaemia in lambs

CASE HISTORY: A 3-month-old female Angus calf was found dead, and two adult Friesian dairy cows died soon after developing nervous signs.

PATHOLOGICAL FINDINGS: Grossly, bilateral and mostly symmetrical areas of haemorrhage were evident that mainly involved areas of grey matter in the brainstem from the level of the caudal colliculi to the thalamus and, in one, the internal capsule and caudate nucleus. In the occipital and caudal parietal cortex, there was extensive oedema of white matter. Histologically, in addition to haemorrhage, there was protein-rich oedema around arterioles and venules in the cerebrum, hippocampus, internal capsule, thalamus, midbrain, dorsal medulla, and central cerebellar and cerebellar folial white matter. The calf's brain had bilateral and symmetrical oedema and necrosis affecting several brainstem nuclei and the occipital grey matter overlying areas of oedema of the corona radiata.

DIAGNOSIS: Although the cause was not established, the perivascular lesions resembled those produced in calves by the intravenous administration of epsilon toxin.

CLINICAL RELEVANCE: It is possible that epsilon toxin-induced enterotoxaemia occurs naturally in cattle, and where bilateral haemorrhage is recognised in the brains of cattle, small intestinal contents should be collected for analysis of epsilon toxin.