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21.
Carter AP Clemons WM Brodersen DE Morgan-Warren RJ Hartsch T Wimberly BT Ramakrishnan V 《Science (New York, N.Y.)》2001,291(5503):498-501
Initiation of translation at the correct position on messenger RNA is essential for accurate protein synthesis. In prokaryotes, this process requires three initiation factors: IF1, IF2, and IF3. Here we report the crystal structure of a complex of IF1 and the 30S ribosomal subunit. Binding of IF1 occludes the ribosomal A site and flips out the functionally important bases A1492 and A1493 from helix 44 of 16S RNA, burying them in pockets in IF1. The binding of IF1 causes long-range changes in the conformation of H44 and leads to movement of the domains of 30S with respect to each other. The structure explains how localized changes at the ribosomal A site lead to global alterations in the conformation of the 30S subunit. 相似文献
22.
Lamb J Crawford ED Peck D Modell JW Blat IC Wrobel MJ Lerner J Brunet JP Subramanian A Ross KN Reich M Hieronymus H Wei G Armstrong SA Haggarty SJ Clemons PA Wei R Carr SA Lander ES Golub TR 《Science (New York, N.Y.)》2006,313(5795):1929-1935
To pursue a systematic approach to the discovery of functional connections among diseases, genetic perturbation, and drug action, we have created the first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules, together with pattern-matching software to mine these data. We demonstrate that this "Connectivity Map" resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs. These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project. 相似文献