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941.
The brain maintains internal models of its environment to interpret sensory inputs and to prepare actions. Although behavioral studies have demonstrated that these internal models are optimally adapted to the statistics of the environment, the neural underpinning of this adaptation is unknown. Using a Bayesian model of sensory cortical processing, we related stimulus-evoked and spontaneous neural activities to inferences and prior expectations in an internal model and predicted that they should match if the model is statistically optimal. To test this prediction, we analyzed visual cortical activity of awake ferrets during development. Similarity between spontaneous and evoked activities increased with age and was specific to responses evoked by natural scenes. This demonstrates the progressive adaptation of internal models to the statistics of natural stimuli at the neural level. 相似文献
942.
Kicheva A Pantazis P Bollenbach T Kalaidzidis Y Bittig T Jülicher F González-Gaitán M 《Science (New York, N.Y.)》2007,315(5811):521-525
In the developing fly wing, secreted morphogens such as Decapentaplegic (Dpp) and Wingless (Wg) form gradients of concentration providing positional information. Dpp forms a longer-range gradient than Wg. To understand how the range is controlled, we measured the four key kinetic parameters governing morphogen spreading: the production rate, the effective diffusion coefficient, the degradation rate, and the immobile fraction. The four parameters had different values for Dpp versus Wg. In addition, Dynamin-dependent endocytosis was required for spreading of Dpp, but not Wg. Thus, the cellular mechanisms of Dpp and Wingless spreading are different: Dpp spreading requires endocytic, intracellular trafficking. 相似文献
943.
Loscertales IG Barrero A Guerrero I Cortijo R Marquez M Gañán-Calvo AM 《Science (New York, N.Y.)》2002,295(5560):1695-1698
We report a method to generate steady coaxial jets of immiscible liquids with diameters in the range of micrometer/nanometer size. This compound jet is generated by the action of electro-hydrodynamic (EHD) forces with a diameter that ranges from tens of nanometers to tens of micrometers. The eventual jet breakup results in an aerosol of monodisperse compound droplets with the outer liquid surrounding or encapsulating the inner one. Following this approach, we have produced monodisperse capsules with diameters varying between 10 and 0.15 micrometers, depending on the running parameters. 相似文献
944.
Nuclear magnetic resonance imaging of the vitreous body 总被引:2,自引:0,他引:2
R G González H M Cheng P Barnett J Aguayo B Glaser B Rosen C T Burt T Brady 《Science (New York, N.Y.)》1984,223(4634):399-400
Imaging with proton nuclear magnetic resonance is a valuable new tool for studying the vitreous body of the eye. It is particularly suited for the detection of vitreal liquefaction and intraocular hemorrhage because of the dependence of the signal on the physical environment of water. Conversely, the vitreous body provides a new model for studying changes in proton relaxation times of protein solutions in biological systems. 相似文献
945.
946.
The main objective of this research was to evaluate two possibilities of material feed rate measurement of modern mowing machines equipped with conditioner. The machines are commonly used for harvest of grasses (Lolium multiflorum, Dactylus glomerata, Phleum pratense, etc.), clover (Trifolium pratense, etc.), alfalfa (Medicago sativa) etc.A mowing machine with finger conditioner was equipped with an electronic measuring unit for the purpose of our measurements. The mowing machine's conditioner shaft was supplied with strain gauges placed on a torque-meter and with a RPM optical sensor counter. Together with torque-meter the mowing machine was equipped with a curved impact plate mounted on the machine's material output.It was decided to arrange a laboratory measurement to obtain information about the dependence of conditioner power input and signals from the impact plate on material mass flow. A mixture of grass and alfalfa was used for our measurement.The measurements carried out proved that a very good linear relationship existed between the conditioner's power input, output frequency of the apparatus measuring impact force by means of the impact plate, and material feed rate through the mowing machine. The calculated coefficients of correlation were about 0.95. It is possible to differentiate a material feed rate difference of 0.5kgs–1 using both methods. This accuracy should be sufficient for practical utilisation such as creating yield maps. 相似文献
947.
Phoberomys is reported to be the largest rodent that ever existed, although it has been known only from isolated teeth and fragmentary postcranial bones. An exceptionally complete skeleton of Phoberomys pattersoni was discovered in a rich locality of fossil vertebrates in the Upper Miocene of Venezuela. Reliable body mass estimates yield approximately 700 kilograms, more than 10 times the mass of the largest living rodent, the capybara. With Phoberomys, Rodentia becomes one of the mammalian orders with the largest size range, second only to diprotodontian marsupials. Several postcranial features support an evolutionary relationship of Phoberomys with pakaranas from the South American rodent radiation. The associated fossil fauna is diverse and suggests that Phoberomys lived in marginal lagoons and wetlands. 相似文献
948.
Dorigo B Schalch T Kulangara A Duda S Schroeder RR Richmond TJ 《Science (New York, N.Y.)》2004,306(5701):1571-1573
Chromatin folding determines the accessibility of DNA constituting eukaryotic genomes and consequently is profoundly important in the mechanisms of nuclear processes such as gene regulation. Nucleosome arrays compact to form a 30-nanometer chromatin fiber of hitherto disputed structure. Two competing classes of models have been proposed in which nucleosomes are either arranged linearly in a one-start higher order helix or zigzag back and forth in a two-start helix. We analyzed compacted nucleosome arrays stabilized by introduction of disulfide cross-links and show that the chromatin fiber comprises two stacks of nucleosomes in accord with the two-start model. 相似文献
949.
Reddien PW Oviedo NJ Jennings JR Jenkin JC Sánchez Alvarado A 《Science (New York, N.Y.)》2005,310(5752):1327-1330
We have identified two genes, smedwi-1 and smedwi-2, expressed in the dividing adult stem cells (neoblasts) of the planarian Schmidtea mediterranea. Both genes encode proteins that belong to the Argonaute/PIWI protein family and that share highest homology with those proteins defined by Drosophila PIWI. RNA interference (RNAi) of smedwi-2 blocks regeneration, even though neoblasts are present, irradiation-sensitive, and capable of proliferating in response to wounding; smedwi-2(RNAi) neoblast progeny migrate to sites of cell turnover but, unlike normal cells, fail at replacing aged tissue. We suggest that SMEDWI-2 functions within dividing neoblasts to support the generation of cells that promote regeneration and homeostasis. 相似文献
950.
Schreier WJ Schrader TE Koller FO Gilch P Crespo-Hernández CE Swaminathan VN Carell T Zinth W Kohler B 《Science (New York, N.Y.)》2007,315(5812):625-629
Femtosecond time-resolved infrared spectroscopy was used to study the formation of cyclobutane dimers in the all-thymine oligodeoxynucleotide (dT)18 by ultraviolet light at 272 nanometers. The appearance of marker bands in the time-resolved spectra indicates that the dimers are fully formed approximately 1 picosecond after ultraviolet excitation. The ultrafast appearance of this mutagenic photolesion points to an excited-state reaction that is approximately barrierless for bases that are properly oriented at the instant of light absorption. The low quantum yield of this photoreaction is proposed to result from infrequent conformational states in the unexcited polymer, revealing a strong link between conformation before light absorption and photodamage. 相似文献