Preventive Medicine and Management in Beef Feedlots

The objectives of beef feedlot preventive medicine are to reduce morbidity, mortality and culling rates, maximize feed efficiency and optimize expenditures for biologicals and pharmaceuticals. The veterinarian must provide regular inspection of all areas of the feedlot and constantly monitor disease incidence. The provision of advice on vaccination, treatment schedules, necropsies on all dead cattle and the regular analysis of disease and production records are all part of feedlot preventive medicine. All of this is aimed at improving net economical gain for the producer.     

Evaluation of a continuous glucose monitoring system in cats with diabetes mellitus

A continuous glucose monitoring system (CGMS) was evaluated in 14 cats with naturally occurring diabetes mellitus. The device measures interstitial fluid glucose continuously, by means of a sensor placed in the subcutaneous tissue. All cats tolerated the device well and a trace was obtained on 15/16 occasions. There was good correlation between the CGMS values and blood glucose concentration measured using a glucometer (r=0.932, P<0.01). Limitations to the use of the CGMS are its working glucose range of 2.2-22.2 mmol/l (40-400 mg/dl) and the need for calibration with a blood glucose measurement at least every 12 h. When compared to a traditional blood glucose curve, the CGMS is minimally invasive, reduces the number of venepunctures necessary to assess the kinetics of insulin therapy in a patient and provides a truly continuous glucose curve.     

Differences in the expression of luteinizing hormone and follicle-stimulating hormone receptors in the lower urinary tract between intact and gonadectomised male and female dogs

Receptors for LH (LHR) and FSH (FSHR) are expressed in the canine lower urinary tract (LUT). As gonadectomy results in an increase in plasma LH and FSH, the objective of this study was to determine whether there are any differences in the expression of LHR and FSHR in the LUT between intact and gonadectomised dogs. Four regions of the LUT, i.e. body and neck of the bladder as well as proximal and distal urethra, were collected from 20 healthy dogs (5 intact males, 5 intact anoestrous females, 4 castrated males and 6 spayed females). The mRNA and protein expression of receptors was determined by in situ hybridization and immunohistochemistry, respectively, and assessed semi-quantitatively incorporating both the distribution and the intensity of specific staining. Expression of LHR and FSHR was present in all tissue layers (epithelium, sub-epithelial stroma and muscle) of each region with different levels of the expression. Overall mRNA and protein expression for both LHR and FSHR was significantly (P < 0.001) lower in gonadectomised dogs. Intact dogs had more (P < 0.05) LHR and FSHR mRNA and protein in all tissue layers of the four regions, except for LHR mRNA expression in the sub-epithelial stroma where no differences were observed between the two statuses. Decreases in LHR and FSHR mRNA and protein in gonadectomised dogs appeared to be more consistent in spayed bitches compared to castrated males. Lower expression of LHR and FSHR observed in gonadectomised dogs may adversely affect the normal canine LUT function.     

Influence of environmental factors on infrared eye temperature measurements in cattle

Environmental factors were evaluated to determine potential limitations in using cattle eye temperatures obtained through infrared thermography (IRT) for early disease detection systems or in animal welfare research studies. The effects of the following factors on IRT eye temperatures in cattle and a fabricated surrogate “eye” were evaluated: camera to object distance, wind speed, camera settings (distance, emissivity, and humidity), and solar loading. Wind speed in both live animals and using a surrogate “eye” was found to decrease the IRT temperature. In the presence of ∼7 km/h wind, the mean IRT eye temperature decreased by 0.43 ± 0.13 °C and; at higher wind speeds (∼12 km/h), the temperature decreased by 0.78 ± 0.33 °C. Direct sunlight was found to increase the IRT eye temperature by 0.56 ± 0.36 °C. It was determined that environmental factors impact IRT temperature measurements significantly and therefore must be managed to ensure reproducible and accurate readings.     

Experimental inoculation of wild turkeys (Meleagris gallopavo) with Mycobacterium bovis

Although avian species are known to be susceptible to infection with Mycobacterium spp. organisms, much remains unknown about the susceptibility of birds to infection with M. bovis. The objective of this current study was to determine if wild turkeys (Meleagris gallopavo) can be infected with M. bovis when inoculated by the oral or intratracheal route. Six turkeys were orally inoculated and another six were inoculated via the trachea with a high dose of M. bovis, 1 x 10(5) CFU/ml. Six turkeys were sham-inoculated controls. Two turkeys from each treatment group were sacrificed on days 30, 60, and 90 postinoculation. There were no gross or microscopic lesions consistent with mycobacteriosis in the 23 inoculated turkeys over the 90-day duration of this study. Fecal cultures were also consistently negative for M. bovis when sampled before inoculation and on days 1, 30, and 60 postinoculation. Two intratracheally inoculated turkeys were positive for M. bovis in visceral tissues at 30 days postinoculation. However, this finding was only indicative of passive persistence of mycobacteria in the tissues and not of infection, as there were no attendant lesions or clinical compromise to support infection. Thus, it can be concluded that young wild turkeys are resistant to infection with M. bovis and, therefore, pose minimal threat as reservoir or spillover hosts for this organism.