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101.
Chun-Ho Park Daisuke Kozima Naoto Tsuzuki† Yoshifumi Ishi†‡ Toshifumi Oyamada 《Veterinary dermatology》2009,20(2):127-130
A solitary mass, 2.5 cm × 2.5 cm × 2.0 cm in size, was found in the subcutis adjacent to the ischial tuberosity of an 8-year-old male German shepherd dog. The excised mass was not encapsulated and the cut surface was solid, grey–white and had multiple red areas suggestive of haemorrhage. Microscopically, the neoplasm was composed of epithelioid cells interspersed with venous vessels of various sizes and irregular bundles of spindle-shaped tumour cells with mucinous stroma. The nuclei showed anisokaryosis and many mitotic figures were noted. Immunohistochemically, the majority of tumour cells were positive for alpha smooth-muscle actin and vimentin, but negative for cytokeratin, desmin, S-100 and factor VIII-related antigen. Ultrastructurally, the tumour cells contained irregular nuclei, few mitochondria, few rough endoplasmic reticulum, cytoplasmic myofilaments of intermediate density, pinocytotic vesicles and distinct basal lamina. Based on these pathological findings, the diagnosis was malignant glomus tumour. 相似文献
102.
Soil microorganisms are major drivers of soil carbon(C) cycling;however,the response of these microorganisms to climate change remains unclear.In the present study,we investigated how 18 months of multifactor climate treatments(warmed air temperature by 3℃ and decreased or increased precipitation manipulation by 30%) affected soil microbial biomass C and nitrogen(N),community substrate utilization patterns,and community composition.Decreased and increased precipitation significantly reduced microbial biomass C by 13.5% and 24.9% and microbial biomass N by 22.9% and 17.6% in unwarmed plots,respectively(P0.01).Warming enhanced community substrate utilization by 89.8%,20.4%,and 141.4% in the natural,decreased,and increased precipitation plots,respectively.Particularly,warming significantly enhanced the utilization of amine and carboxylic acid substrates among all precipitation manipulation plots.Compared with the natural air temperature with natural precipitation treatment,other treatments affected fungal community richness by -0.9% to 33.6% and reduced the relative abundance of the dominant bacterial and fungal groups by 0.5% to 6.8% and 4.3% to 10.7%,respectively.The warming and/or precipitation manipulation treatments significantly altered Zygomycota abundance(P0.05).Our results indicate that climate change drivers and their interactions may cause changes in soil microbial biomass C and N,community substrate utilization patterns,and community composition,particularly for the fungal community,and shifts in the microorganism community may further shape the ecosystems function. 相似文献
103.
104.
PARK NAM‐IL MASARU OGASAWARA KOICHI YONEYAMA YASUTOMO TAKEUCHI 《Weed Biology and Management》2001,1(4):222-225
The growth response to fertilizer under different soil texture (light clayey soil and composite soil consisting of the light clayey soil and sand), and the timing of N fertilization (early summer and fall fertilization) were determined for annual bluegrass (Poa annua L.) and creeping bentgrass (Agrostis palustris Huds., var. Penncross) in glasshouse experiments. At the three‐leaf stage, both plant species were treated with 30, 100, 300, 1,000, 3000 or 10 000 p.p.m. (w/v) of N, P2O5 and K2O equivalent to ammonium sulfate (N), calcium superphosphate (P) and potassium chloride (K). Three weeks after fertilization, the dry weight of shoots or roots was measured. Both plant species were more sensitive to N than to P or K; however, creeping bentgrass required a higher rate of N (1000 p.p.m.) for maximum shoot and root growth than did the weed (300 p.p.m., N). Shoot growth of both plant species was strongly affected by the timing of N fertilization and soil texture; their shoot responses to N increased in the order: composite soil > light clayey soil, and early summer N fertilization > fall N fertilization, and this tendency was especially marked for creeping bentgrass. These results suggest that the response of annual bluegrass to fertilizer is similar to that of creeping bentgrass; however, the two plants are different in regard to the N requirement or their relationship between response to N and application timing and/or soil texture. 相似文献
105.
Hassadin BOONSRIROJ Daria Llenaresas MANALO Kazunori KIMITSUKI Taichi SHIMATSU Nozomi SHIWA Harumi SHINOZAKI Yurika TAKAHASHI Naoto TANAKA Satoshi INOUE Chun-Ho PARK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):35-42
Rabies is a zoonotic disease caused by the rabies virus. While the salivary glands are
important as exit and propagation sites for the rabies virus, the mechanisms of rabies
excretion remain unclear. Here, we investigated the histopathology of the salivary glands
of rabid dogs and analyzed the mechanism of excretion into the oral cavity. Mandibular and
parotid glands of 22 rabid dogs and three control dogs were used. Mild to moderate
non-suppurative sialadenitis was observed in the mandibular glands of 19 of the 22 dogs,
characterized by loss of acinar epithelium and infiltration by lymphoplasmacytic cells.
Viral antigens were detected in the mucous acinar epithelium, ganglion neurons and
myoepithelium. Acinar epithelium and lymphocytes were positive for anti-caspase-3
antibodies and TUNEL staining. In contrast, no notable findings were observed in the
ductal epithelial cells and serous demilune. In the parotid gland, the acinar cells,
myoepithelium and ductal epithelium all tested negative. These findings confirmed the path
through which the rabies virus descends along the facial nerve after proliferation in the
brain to reach the ganglion neurons of the mandibular gland, subsequently traveling to the
acinar epithelium via the salivary gland myoepithelium. Furthermore, the observation that
nerve endings passing through the myoepithelium were absent from the ductal system
suggested that viral proliferation and cytotoxicity could not occur there, ensuring that
secretions containing the virus are efficiently excreted into the oral cavity. 相似文献
106.
Yong Kwan KIM Seong-In LIM Jae-Jo KIM Yoon-Young CHO Jae-Young SONG In-Soo CHO Bang-Hun HYUN Sung-Hyun CHOI Seung-Hoe KIM Eun-Hye PARK Dong-Jun AN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1667-1671
Classical swine fever (CSF) is a highly contagious systemic hemorrhagic viral disease of
pigs. Wild boar plays a crucial role in the epidemiology of CSF. Between 2010 and 2014,
samples were collected nationwide from 6,654 wild boars hunted in South Korea. Anti-CSF
antibodies were identified in 0.59% (39 of 6,654) of the wild boar samples using a virus
neutralization test and were primarily detected in wild boars living close to the
demilitarized zone and the area of the Taebaek Mountains surroundings. The CSF virus
(subgroup 2.1b) was isolated from two wild boars captured in a nearby border area. The
criteria used to define high-risk areas for targeted CSF surveillance in South Korea
should be further expanded to include other regions nationwide. 相似文献
107.
108.
硅肺是吸入SiO2粉尘引起的以肺间质纤维化为主的全身性疾病。探讨了白茶提取物对纳米SiO2诱导大鼠肺纤维化的抑制作用及机制。54只Wistar大鼠随机分为正常组、模型组、白毫银针提取物组、白牡丹提取物高和低剂量组、EGCG组共6个组,每组9只大鼠。除正常组外的其余5个组采用非暴露式气管插管方法造模纳米SiO2粉尘(80 mg·mL-1),每天以灌胃方式给予药物两周之后,检测肺组织中羟脯氨酸(HYP)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)和谷光甘肽过氧化物酶(GSH-Px)含量以及肺组织形态学变化。结果显示,与模型组相比,白茶提取物各处理组及EGCG组病理形态改变有不同程度的减轻,其中白毫银针提取物组的效果最佳。白茶提取物各处理组与EGCG组的大鼠肺NO含量和炎症因子IL-6都显著低于模型组(P<0.05),GSH-Px活力高于模型组(P<0.05),高剂量白牡丹提取物对降低NO含量和升高GSH-Px活力效果最好;本研究表明,白茶提取物对于纳米SiO2引起的大鼠肺纤维化氧化应激损伤具有有效的减缓和修复作用,主要与其抗氧化作用和抑制炎症反应相关。 相似文献
109.
肉鸡生产中通过抗生素来促进生长和提高饲料效率会存在一定程度的安全隐患,所以欧盟已通过立法禁止这类抗生素的使用。在美国,越来越多的消费者希望生产者能提供不饲喂抗生素的家禽产品。尽管已经找到了很多可以部分或全部发挥抗生素功能的替代品,但是,还没有一种能够在养殖生产中得到广泛应用。 相似文献
110.
MARTHA G. DRUM CHRISTOPHER E. KAWCAK ROBERT W. NORRDIN RICHARD D. PARK C. WAYNE McILWRAITH CLIFFORD M. LES 《Veterinary radiology & ultrasound》2007,48(6):518-527
Comparison of subchondral bone density determined by quantitative computed tomography (CT) with gross and histopathologic changes have not been made in horses. The goal of this study was to determine if mean quantitative CT density and mean voxel standard deviation are associated with the presence and severity of osteochondral lesions in the palmar aspect of the distal third metacarpal bone in racing horses. Metacarpophalangeal joints from nine racehorses were imaged using CT and scored for gross damage. Four-millimeter-thick sagittal and 30 degrees palmar dorsal plane sections were cut, decalcified and stained with hematoxylin and eosin from the distal third metacarpal bone. Microscopic osteochondral lesions and subchondral remodeling were scored on a scale of 0-3. Percent subchondral bone, expressed as the ratio of bone volume to tissue volume, was also measured. Mean quantitative CT density and mean voxel standard deviation were measured from three-dimensional models of CT images comparable with histologic sections. Mean quantitative CT density was not associated with lesion severity or number of lesions. A weak correlation between mean quantitative CT density and gross score was found, but mean quantitative CT density was not predictive for gross score. Mean voxel standard deviation was not correlated with gross or histopathologic measures, but was predictive of mild osteochondral lesions. Results support the association of subchondral remodeling with the development of palmar metacarpal lesions. However, there was not a strong correlation between mean quantitative CT density or mean voxel standard deviation and histopathologic lesions of the distal third metacarpal bone. 相似文献