In vivo kinetic study on uptake and distribution of intramuscular tritium-labeled polysulfated glycosaminoglycan in equine body fluid compartments and articular cartilage in an osteochondrial defect model

The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (³H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. ³H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean ³H-PSGAG concentrations peaked at 2 hours post-injection. ³H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in ³H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.

HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.

³H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan.     

In vitro viability of cryopreserved equine embryos following different freezing protocols.

The main objective of this study was to evaluate two freezing protocols and the effect of agar embedding on survival of day 6.5 equine embryos. A total of 133 embryos were used, in one group (n = 51), embryos were first embedded in agar before the freezing protocol was started. A freezing protocol to -30 degrees C or -33 degrees C was used before plunging embryos into liquid nitrogen (LN2). The embryos were thawed in water at 37 degrees C, evaluated and placed in culture. After 24 h culture, the embryos were evaluated for their morphology and development. No differences were observed between embryos plunged at -30 degrees or at -33 degrees C in LN2. The analysis of the morphology and development after thawing showed that the diameter and developmental stage at freezing correlated with embryo survival. Morula and early blastocyst stages of development were associated with better quality after freezing and thawing and had a better potential to survive after in vitro culture (p < 0.05) compared to more advanced stages. The agar failed to protect embryos from zona pellucida damage, but a tendency to prevent rupture was observed in larger embedded embryos.     

沙区五种珍稀濒危植物水分生理指标测定及分析

本文在测定沙区５种珍稀濒危植物的水分生理指标的基础上，分析了其抗旱性，意在探讨适应干旱环境的特性。研究结果表明：矮沙冬青保水能力与吸水能力强，对干燥炎热的环境有很强的适应性；银沙槐蒸腾强度大，失水快，但吸水力强，补偿水分快；裸果木通过强的保水力适应干旱；绵刺以强的吸水力和抗脱水力适应环境；准噶尔无叶豆以体内水分自我调节作用适应大气干旱。     

水稻不育系粒黑粉病普遍率与严重度间的关系

 稻粒黑粉病(病原菌Neovossia horrida (TaK.)Padw. et A. Kahn)是杂交稻繁殖制种田不育系上的主要病害,流行频率高,危害损失大。普遍率和严重度的关系(I-S关系)是植病流行学观测技术上的一个重要问题。     

荔枝采后果皮变色机理研究

对不同采后处理的荔枝在贮藏过程中果皮的渗透率和多酚氧化酶活性变化进行了研究，结果表明：当果皮细胞膜渗透率大于30％时。荔枝果皮就发生大面积褐变；果皮细胞膜渗透率的变化是荔枝采后果皮变色的一个重要指标，而多酚氧化酶活性的高低并不是荔枝果皮颜色变化的决定因素。     

Effects of aminoglycoside antibiotics on renal antioxidants, malondialdehyde levels, and some serum biochemical parameters

Effects of amikacin, gentamicin, kanamycin, and streptomycin on renal tissue superoxide dismutase, glutathione peroxidase, glutathione and malondialdehyde, serum creatinine, potassium, sodium, total protein, glucose, uric acid, and total bilirubin levels were investigated. All aminoglycoside antibiotics decreased renal tissue glutathione levels.     

灰紫香蘑的分离培养

对灰紫香蘑(Lepista glaucocana)在14种培养基上进行了分离培养研究,获得了纯菌种。在PDA、燕麦、黄豆粉培养基上菌落颜色为紫色,是灰紫香蘑分离培养的最佳培养基;液体培养基配方筛选表明,PD培养基为长势最佳的液体配方;在固体扩繁培养基上,草炭78% 麦麸20%是最适合的培养基质,经2个月的生长,可长满果酱瓶。     

Presentation of postweaning Escherichia coli diarrhea in southern Ontario, prevalence of hemolytic E. coli serogroups involved, and their antimicrobial resistance patterns

Post-weaning Escherichia coli diarrhea (PWECD) in Ontario was investigated using a case-control study involving 50 Ontario nurseries. The clinical signs and the impact on productive parameters were determined by means of a producer survey. The hemolytic E. coli serogroups involved in PWECD (O149:K91:K88) were examined in this study. Based on a polymerase chain reaction test, the hemolytic E. coli from 82% of the case herds were positive for 3 enterotoxins (STa, STb, and LT), those from 12% of the case herds were positive for STb and LT only, and those from one herd (6%) were positive for 3 enterotoxins, as well as for verotoxin and F18 pili. The E. coli involved in disease were resistant to multiple antibiotics. Case farms commonly used a wide variety of antibiotics either in the feed or water, or as injectable drugs. The most common antibiotic used to treat PWECD on the study farms was apramycin, but evidence of resistance to this antibiotic was noted. The PWECD problem was commonly seen within a week of weaning but onset of diarrhea was reported as late as the grower-finisher stage. Growth rate was poorer in case herds and mortality was higher than in control herds, demonstrating that PWECD is an economically important disease in Ontario.