Electrophoresis Study of Extracts of Submaxillary Salivary Glands from Naturally Rabid Dogs

Rabies virus in submaxillary salivary glands from naturally infected dogs was investigated by a paper electrophoresis technique, and the virus activity was quantitated by intracerebral titration in mice. Extracts from these salivary glands were found to contain (a) 10⁴ to 10⁶ mouse ICLD ₅₀ units of wild rabies seed and (b) a protein complex that migrated electrophoretically in the albumin band and more conspicuously in the beta and gamma bands of normal horse serum. The protein complex was interpreted to comprise aggregates of neutralizing antirabies antibody.     

世界猪病的发展与演变(续)

17 群体水平上的复合病因网络 在集约化生产系统中,我们面临的大多数疾病都有着复杂的成分因素。其中一些可能是必要因素,如果没有他们的存在疾病也就无从发生,但当他们独自起作用时却并不是充分原因。“Koch’s”假设有其局限性,只有对疾病病因作现场检验时才起作用。那些病原单独存在并且是必要充分原因的疾病通常是最易     

Effects of protein concentration on responses to dietary lysine by chicks

Chicks were fed on diets varying in crude protein (CP) content (140 to 280 g/kg diet) in either 8 steps, experiment 1, or 6 steps, experiment 2. Protein composition was held constant in each experiment. At each protein concentration, 5 (experiment 1) or 6 (experiment 2) concentrations of lysine were tested, ranging from 40 to 60 g/kg CP. Growth rate and efficiency of food utilisation to 21 d of age responded to increasing dietary protein contents up to about 230 g CP/kg. An estimate of lysine requirement at each protein concentration was obtained by fitting a quadratic curve to the response data and calculating the dose of lysine (g/kg CP) needed to maximise either growth rate or gain/food ratio. Although no growth response to dietary protein was obtained between 240 and 280 g CP/kg, the amount of lysine needed to maximise growth and gain/food ratio over this range increased systematically when expressed as g/kg diet, but remained constant if expressed as g/kg CP. The regression of lysine required (g/kg diet) for maximum performance (growth or food efficiency) on CP (g/kg diet) was strictly linear for both responses in both experiments throughout the entire range studied (140 g CP/kg to 280 g CP/kg). The estimated lysine requirement was 0.053 of the CP in experiment 1 and 0.055 of the CP in experiment 2. It is concluded that a fixed ratio of lysine to protein should be specified in practical diet formulation, rather than a minimum dietary concentration of lysine. This would ensure that, if the dietary protein content rises above a prescribed minimum value in least-cost formulation, an appropriate adjustment will automatically be made to the lysine content of the solution.     

Enzootic pneumonia: comparison of cough and lung lesions as predictors of weight gain in swine.

A prospective cohort study was undertaken in a commercial swine herd to determine the relationship between weight gain and the occurrence of enzootic pneumonia (EP). Estimates of the association between EP and weight gain were obtained from multiple linear regression models, using coughing episodes or slaughter checks as indicators of EP status. Models were estimated for pigs in 2 different age groups: roasters (n = 1084, sold at 32 to 50 kg) and market hogs (n = 1162, sold at 100 to 110 kg). The relationship between presence of lung lesions at slaughter and previous coughing episodes was also investigated. Throughout the study period, clinical evaluations were performed weekly, and coughing episodes recorded for each pig. Lungs were inspected at slaughter, and scores were expressed as the percentage of the lung with gross pneumonic lesions. Coughing and lung scores were significantly correlated, after adjusting for other covariates (R = 0.32 and 0.59, respectively, for market hogs and roasters). However, the agreement beyond chance between coughing history and lung lesions at slaughter was poor among both roasters and market hogs (kappa = 0.17 and 0.07, respectively). Although very specific, weekly assessment of coughing was not a sensitive indicator of lung lesions at slaughter. In multiple regression, lung score was a highly significant predictor of lower final weight in pigs of both age groups (P < 0.001 in the selected regression models).(ABSTRACT TRUNCATED AT 250 WORDS)     

Estimate of economic impact of fowl cholera in turkeys in Georgia in 1986

Information gathered from cases of fowl cholera (FC) in commercial turkey flocks through case records, flock records, and telephone and mail surveys was used to estimate disease costs. The cost to the Georgia commercial turkey industry in 1986 from preventive measures, treatment of outbreaks, and production losses from the disease was estimated at $634,545. The cost of FC per kg of live production was estimated to be $0.015.     

Shared epitopes between mycobacteria and other microorganisms

Epitopes of antigens from a range of bacteria, parasites and fungi were identified by monoclonal antibodies as being similar to epitopes from mycobacterial antigens. Antibodies to many of these cross-reacting epitopes are present in normal mammalian and avian sera.     

Time of lay in hens exposed to intermittent lighting

1. Two short-term trials are described in which laying hens were exposed to 8 h light followed by 8 one-min pulses of light at hourly intervals followed by 8 h darkness (8L:8i:8D). The effect of varying the intensity of illumination during the one-min pulses and the effect of placing the intermittent lighting before the 8 h photoperiod (8i:8L:8D), were studied. 2. Normal egg production was maintained by the 8L:8i:8D system when the light pulses were at 20 lux, but not at 5 lux. This suggests a minimum threshold for illumination with short light pulses higher than that needed for continuous lighting. 3. Time of lay under 8L:8i:8D was the same as with 8L:16D in relation to the beginning and ending of the 8 h main photoperiod, but with 8i:8L:8D mean time of lay was 2 to 3 h earlier. Thus the hourly pulses caused a phase advance when placed before the normal photoperiod but did not cause a phase delay when placed after the normal photoperiod.     

The determination of the methionine requirement of laying pullets by a diet dilution technique

A method for the determination of amino acid requirements of laying hens is described. This involves the dilution of a high protein “ summit” diet with an isocaloric nitrogen‐free mixture. By ensuring that the amino acid to be assayed is first‐limiting in the summit diet, the response to dilution can be interpreted as a response to a single amino acid.

The method is applied to the determination of methionine requirement and it is shown that the response to methionine obtained is virtually independent of the protein level of the diet and is not influenced by direct effects of the dilution mixture.

From the results of the present and other published experiments, the “ available “ methionine required for maximum egg yield of pullets in the early stages of lay is estimated to be 275 mg per bird per day.