Olfactory and quantitative analysis of aroma compounds in elder flower (Sambucus nigra L.) drink processed from five cultivars

Fresh elder flowers (Sambucus nigra L.) were extracted with an aqueous solution containing sucrose, peeled lemon slices, tartaric acid, and sodium benzoate to make elder flower syrup. Aroma compounds emitted from the elder flower syrup were collected by the dynamic headspace technique and analyzed by GC-FID and GC-MS. A total of 59 compounds were identified, 18 of which have not previously been detected in elder flower products. The concentrations of the identified volatiles were measured in five elder cultivars, Allesoe, Donau, Sambu, Sampo, and Samyl, and significant differences were detected among cultivars in the concentration levels of 48 compounds. The odor of the volatiles was evaluated by the GC-sniffing technique. cis-Rose oxide, nerol oxide, hotrienol, and nonanal contributed to the characteristic elder flower odor, whereas linalool, alpha-terpineol, 4-methyl-3-penten-2-one, and (Z)-beta-ocimene contributed with floral notes. Fruity odors were associated with pentanal, heptanal, and beta-damascenone. Fresh and grassy odors were primarily correlated with hexanal, hexanol, and (Z)-3-hexenol.     

Nitrous oxide emission from a soil under permanent grass: Seasonal and diurnal fluctuations as influenced by manuring and fertilization

The emission of nitrous oxide (N₂O) from soil under grass was measured, following applications of cow slurry and NH₄NO₃ fertilizer. The N₂O-flux from untreated soil averaged 0.58 mg Nm^?2 day^?1 through April to August. Application of slurry at the end of April and at the middle of July caused increases in the daily N₂O-flux of up to 40-fold, compared to untreated grass. Applications of NH₄NO₃ increased the N₂O-flux up to 5 times during the same period. The N₂O-flux often showed marked diurnal fluctuations. These fluctuations are not solely associated with change in temperature, but may also be related to grass root activity and to photosynthesis.     

溴虫腈与哒螨灵对朱砂叶螨的联合作用

为明确溴虫腈与哒螨灵的联合杀螨活性,采用玻片浸渍法测定了溴虫腈与哒螨灵不同配比混剂对朱砂叶螨雌成螨的杀螨活性,并以共毒因子法和共毒系数法评价混剂的增效作用。结果表明,溴虫腈和哒螨灵对朱砂叶螨雌成螨处理后24 h的致死中质量浓度(LC50)分别为8.80、17.69mg/L;溴虫腈与哒螨灵的质量比为49∶51、47∶53、45∶55时增效最明显,对朱砂叶螨处理后24 h的共毒系数分别为177.94、178.49、173.73,LC50分别为6.65、6.72、7.00 mg/L,其毒力分别是哒螨灵的2.66倍、2.63倍、2.53倍。因此,45%~49%溴虫腈与51%~55%哒螨灵混合具有显著的增效作用。     

家庭因素对边疆少数民族大学生心理健康影响研究

为准确把握家庭因素对边疆少数民族大学生心理健康的影响,本文运用卡特尔16PF人格因素量表、大学生人格问卷UPI和SCL-90症状自评量表,采用随机抽样的方式,对边疆9省少数民族大学生心理健康状况进行调查;并结合自己设计的《边疆地区少数民族大学生基本情况调查问卷》的调查结果进行对应分析,用SPSS13.0进行数据处理。结果表明:家庭整体氛围、家庭教育方式与家庭成员的关系状况对研究对象的心理健康状况有显著影响,而家庭规模、家庭生活水平、家庭收入水平、家庭负担大学开支情况,以及研究对象是否是独生子女、在家中的排行、在家庭中的地位对研究对象的心理健康状况没有显著影响。     

Refrigerated Storage of Channel Catfish Sperm

Abstract.— Refrigerated storage of sperm is useful for genetic study and artificial breeding of fishes. Due to the potential loss of donor males, storage is important in species such as channel catfish Icralurus punctatus from which sperm cannot be stripped. This study addresses short-term storage (at 4 C) of channel catfish sperm by evaluation of storage methods employed for other species and for cryopreservation of channel catfish sperm. The objectives were to evaluate: 1) storage of intact testes and storage of sperm suspended in an extender solution; 2) use of various storage containers with and without supplemental oxygen; 3) use of extender solution with and without the addition of an antibiotic/antimycotic cocktail; 4) use of extender solution with and without the addition of methanol; and 5) use of extender solution with and without the addition of glucose and methanol. Sperm suspended in extender solution retained motility significantly longer (9 d) than did sperm in intact testis (2 d). Sperm stored in Zip-loc® plastic bags inflated with pure oxygen retained motility significantly longer (12 d) than did sperm stored in Zip-loc® plastic bags without supplemental oxygen (7 d), or sperm stored in plastic beakers (8 d) or test tubes (8 d) without supplemental oxygen. Sperm stored with the addition of antibiotic/antimycotic cocktail or methanol retained motility significantly longer (10–12 d) than did sperm stored without additives (6–8 d). Sperm stored in extender solution without glucose retained motility significantly longer (19–21 d) than did sperm stored in extender with glucose (13–16 d). Motility was retained for as long as 21 d in sperm stored in extender solution with 5% methanol and without glucose. In each experiment, loss of motility was associated with bacterial growth.     

The growth rate and the variation in growth in relation to the stock plant of Hedera canariensis Willd. ‘Gloire de Marengo’

When leaf-bud cuttings from Hedera canariensis Willd. ‘Gloire de Marengo’ were prepared with various stem lengths, the subsequent shoot length was inversely correlated with the stem length below the axil. The shoot growth was also correlated with the size of the leaf of the cutting but not by the position along the stem of the stock plant. Certain stock plants yielded cuttings with significantly greater shoot lengths than did others.The shoot length obtained was variable (coefficient of variation 36%), and this variation was reduced neither by standardising the length of the stem below the axil, nor by standardising the area of the original leaf on the cuttings. However, some stock plants produced less variable cuttings than others, and variability was shown to be inversely correlated with the shoot length of the propagated plants.     

Cutaneous neoplasms in dogs associated with canine oral papillomavirus vaccine

A spectrum of proliferative cutaneous lesions occurred in 12 dogs at the injection site of live canine oral papillomavirus (COP) vaccine, suggesting a viral etiology for the masses. Lesions included epidermal hyperplasia, epidermal cysts, squamous papilloma, basal cell epithelioma, and squamous cell carcinoma. Peroxidase-antiperoxidase staining of tumor sections revealed nuclei which stained for group-specific papillomavirus antigen in five of 12 masses. Electron microscopic examination of tumor sections did not reveal virions. In transmission studies, macerated tumor tissue did not produce oral papillomas on the scarified mucosa of puppies; this procedure did protect the puppies from development of lesions when challenged with infectious papilloma material. These findings are evidence that COP can induce hyperplastic and neoplastic lesions in sites other than oral, pharyngeal, and ocular mucosa.     

Attempts to immunize cattle against Ostertagia ostertagi infections employing 'normal' and 'chilled' (hypobiosis-prone) third stage larvae.

Immunity to Ostertagia ostertagi infections in calves develops slowly and only becomes manifest towards the end of a grazing season in which they have been exposed to the parasite. In an attempt to hasten the onset of immune reactions, three immunization protocols were set up. Twenty four heifers were allocated into four groups. Beginning in January, animals in two of the groups were inoculated with four 1-monthly increasing dosages of either 'normal' or 'chilled' (hypobiosis-prone) larvae, those in the third group received a single large infection with 'chilled' larvae and those in the fourth group served as non-infected controls. All animals were turned out on a common pasture in late April. Development of immunity was evaluated through determinations of faecal egg counts, live weight gains, serum pepsinogen levels and specific serum antibody responses of three isotypes (IgG1, IgG2 and IgA). Significantly reduced egg excretions in the immunized groups were apparent early in the season, indicating that the immunizations had, in this respect, been efficacious. The 'chilled' and 'normal' larvae seemed equally efficient given as multiple and single infections. A single large dosage of 'chilled' larvae seemed to have adverse effects. Only moderate antibody responses were elicited probably because of low challenge infection level on pasture. Considerable variation in responses existed between and within the four groups, for which reason conclusions regarding correlations between antibody isotype responses and immune effects on parasites could not be made.