Organic Halogens in Landfill Leachates

Using a group parameter, totalorganic halogens (TOX), high TOX concentrationswere found in leachates and leachate contaminatedgroundwaters at two Danish mixed sanitary andhazardous waste sites. With commonly used screeningprocedures for organic contaminants, the individualhalogenated organic compounds behind the high TOXcould not be found. Conversely, part of the TOX couldbe attributed to halogenated humic substances that cannot be analysed with conventional organic contaminantanalyses. Even though TOX can still be considered anindicator of leachate contamination in the aquifers,attempts to identify the halogenated contaminantsbehind the TOX using screening methods such as GC-MSwill be unsuccessful in most cases.     

The effects of creep feed composition and form and nursery diet complexity on small intestinal morphology and jejunal mucosa-specific enzyme activities after weaning in pigs

Fifty-six litters from first-parity sows standardized to 12 piglets were used to determine the effects of creep feed composition and form and the provision of low- or high-complexity nursery diets on the evolution of small intestinal histomorphology and jejunal mucosa-specific enzyme activities postweaning. At 5 d of age, litters (initial bodyweight [BW] 2.31 ± 0.61 kg) were assigned to one of four creep feeding regimens (n = 14): 1) commercial creep feed (COM), 2) liquid milk replacer (LMR), 3) pelleted milk replacer (PMR), or 4) no creep feed (NO). At weaning (21 d of age), six pigs per litter were provided a HIGH- (contained highly digestible animal proteins) or LOW- (contained corn and soybean meal as main protein sources) complexity nursery diet (n = 7). At 21, 28, and 59 d of age, two pigs per pen (one castrated male and one female) were euthanized, and ileal and jejunal segments for histomorphological measurements and jejunal mucosal scrapings were collected to determine specific mucosa enzyme activities. At weaning, pigs provided COM had a greater ileal absorptive capacity (M) than LMR or NO, which were not different (14.1 vs. 10.4 and 10.5 ± 0.9 μm²; P < 0.05); PMR was intermediate. On days 28 and 59, M was not different among pigs regardless of creep feed treatments. Pigs fed LOW had reduced jejunal villus height (VH; P < 0.001) and M (P < 0.001) on day 28 vs. day 21. The VH and M were not different for pigs fed HIGH or LOW by the end of the nursery period. For all dietary treatments except COM-HIGH and COM-LOW, jejunal mucosal maltase-specific activity was not different between days 21 and 28 of age but greater on day 59 (P < 0.05). For pigs that received COM-HIGH, maltase-specific activity was not different between days 21 and 28 but greater on day 59 than day 28 (P < 0.05). For pigs that received COM-LOW, maltase-specific activity was not different between days 21, 28, and 59. Regardless of creep or nursery treatment, sucrase-specific activity was the greatest on day 59, followed by days 21 and 28 (P < 0.001), and lactase-specific activity was greater on day 21 than on days 28 and 59 (P < 0.001), which were not different. Therefore, pigs that provided LOW diet had greater villus atrophy and reduced M during the first week after weaning vs. pigs that provided HIGH, regardless of creep feeding regimen, but were able to recover by the end of the nursery period.     

A correction factor for,and its application to,visual censuses of littoral fish

Visual estimation techniques were used between October 1976 and May 1977 on the fish fauna of an enclosed mid-intertidal pool in the eastern Cape, South Africa, to test the usefulness and accuracy of this non-consumptive census method. In January, 1977, two pools were censused for fish, then poisoned using the ichthyocide ‘pronoxfish’ and all fish collected. Forty species in 21 families were obtained from the two stations. The accuracy of visual estimates varied with the species of fish. Secretive and cryptically coloured types, as well as species which Inhabit crevices, were underestimated, between 0 and 86% being counted. Other fish with protective colours but that occur more openly and do not react adversely to divers, as well as schooling species, were also underestimated, but up to 100% of the total were observed. Those which occur singly or in small groups in the water column were counted more accurately, with 57 to 100% of the actual number being seen. Correction factors were calculated from these data and applied to a census taken of one pool in May. Comparisons of corrected and actual numbers Indicate that these factors were relatively accurate for non-secretive species. The factors will, however, vary for each species, from observer to observer, depending on their personal experience, and from area to area.     

Salmonella in pork cuttings in supermarkets and butchers' shops in Denmark in 2002 and 2006

The prevalence of Salmonella in fresh pork cuttings in Denmark in the years 2002 and 2006 was investigated at retail and compared with the retail supply pattern. A total of 1025 and 3473 samples were taken in 2002 from butcher's shops and supermarkets, respectively. The corresponding numbers in 2006 were 259 from butchers' shops and 628 from supermarkets. In 2002, 1.2% of all samples were positive for Salmonella; butchers' shops and supermarkets had 1.8% and 1.0% positive samples, respectively. The overall prevalence in 2006 was 4.2%, with prevalence of 8.1% and 2.6% for butchers' shops and supermarkets, respectively. Hence, increases around 3- to 5-fold were found. There was neither observed any parallel increase in Salmonella positive carcasses in Danish slaughterhouses during the study period, nor were any changes in supply routes towards slaughterhouses with higher prevalence observed, which could explain the apparent increase. We hypothesize that hygiene levels and ability to avoid cross-contamination and prevent growth of the organism, in the meat processing chain after slaughter were the most likely responsible factors. Results from this study indicate that the hygiene performance, particularly at retail, has a significant impact on the occurrence of Salmonella. This implies that there is no direct link between slaughterhouse Salmonella surveillance data and the level of Salmonella contamination at retail. To improve risk assessment of Salmonella in fresh pork meat, this study underlines the need for comprehensive retail data.     

Long‐term effects of animal manure and mineral fertilizers on phosphorus availability and silage maize growth

A better appraisal of the plant availability of soil phosphorus (P) added with animal manure is crucial to alleviate environmental impacts from over‐application of P. This study compares the availability of P to maize in the Askov long‐term experiments using unmanured plots and plots receiving corresponding rates of nitrogen (N), P and potassium (K) in mineral fertilizers or manure. Total‐P and water extractable P (Pw) in soil, and plant height, dry weight, P concentration and P uptake were determined in early August. Final yields were determined in late October. Soil Pw was similar for plots receiving corresponding rates of P in mineral fertilizer or manure form. With a strong relationship between Pw, and maize growth and final yields, Pw was a reliable indicator of P availability to maize. Plant dry weight, P concentration and P uptake in early August were 23%, 8% and 31% higher, respectively, for maize grown on soil receiving manure compared with mineral fertilizer, while final maize yield in late October was 13% higher. Plant height and dry weight determined in early August suggested that maize development at this growth stage defined final maize yield. We conclude that the availability of P was similar after long‐term application of corresponding rates of P in animal manure and mineral fertilizers, and that animal manure improves the growth of maize compared to mineral fertilizers. This is ascribed to micronutrients and residual N effects from previous additions of manure.     

Suppression of Babesia microti infection in mice concurrently infected with Fasciola hepatica

Blood cell parasitaemia of Babesia microti and the associated haematological changes were examined in mice harbouring patent Fasciola hepatica infections and in fluke-free control mice. B. microti parasitaemia was markedly suppressed in mice harbouring primary 7-week F. hepatica infections, as reflected in a reduction in the percentage of erythrocytes parasitised and in the incidence of multiple B. microti infections in the red cells. This suppression was accompanied by an annulment of B. microti induced reductions in haemoglobin and haematocrit levels in F. hepatica infected mice. In naive recipient mice, inoculated with blood from mice concurrently infected with F. hepatica and B. microti, the course of B. microti infection was characterised by a prolonged pre-parasitaemia period, a reduced peak parasitaemia and a delayed fall in haematocrit levels as compared to those inoculated with blood from mice infected with B. microti only. This feature may presumably be dose-related. The present study does not reveal the actual mechanism(s) involved in the suppression of the blood protozoan by F. hepatica. However, since B. microti has a preference for mature erythrocytes, the suppression may be a result of the altered erythrocyte kinetic state induced by the removal of erythrocytes by the blood-sucking fluke resulting in high levels of reticulocytes.     

Effects of flaxseed supplementation on milk production, milk fatty acid composition and nutrient utilization by lactating dairy cows.

Twelve multiparous Holstein cows at 72 +/- 20 days in milk were used in a switch-back design with 14-d periods to determine the effect of replacing barley grain into a dairy total mixed ration with micronized or raw flaxseed on nutrient digestibility, milk yield, milk composition. Total mixed diets were (DM basis) 50% barley silage, 50% concentrate mix mainly rolled barley grain and canola meal. Diets were supplemented with 1 kg raw (RF) or micronized (MF) flaxseed to substitute 1 kg of rolled barley grain (C). Neutral detergent fibre, ADF and CP digestibility of the diets were not significantly affected by supplementation; however, calcium digestibility was reduced by 62% and 46% when raw and micronized flax were fed, respectively. Milk yield (38.3, 39.6, and 38.4 kg/d for diets C, RF and MF, respectively) was similar for all diets. Milk fat (3.50, 3.48, and 3.52%) and protein (3.31, 3.34, and 3.31%) for diets C, RF and MF, respectively, were not affected by treatment diets. Concentrations of c9, t11 conjugated linoleic acid (CLA; 0.51, 0.72 and 0.76 g/100 g fatty acids) in milk fat increased (P<0.05) similarly among the two flaxseed supplemented diets. The RF and MF diets significantly increased the C18:1, C18:1 trans-11, C18:2 cis-9, cis-12 and C18:3 in milk fat however, C12:0, C14:0 and C16:0 were significantly reduced compared with control. Replacing barley grain with flaxseed in the diet of lactating cows increased the beneficial fatty acids in milk without depressing nutrient digestibility. Micronization of flaxseed did not reveal any advantage over raw flaxseed.     

Profile of selected bacterial counts and Salmonella prevalence on raw poultry in a poultry slaughter establishment.

The USDA Food Safety and Inspection Service determined populations of bacteria on poultry during processing at a slaughter plant in Puerto Rico in November and December 1987. The plant was selected because of its management's willingness to support important changes in equipment and processing procedures. The plant was representative of modern slaughter facilities. Eight-hundred samples were collected over 20 consecutive 8-hour days of operation from 5 sites in the processing plant. Results indicated that slaughter, dressing, and chilling practices significantly decreased the bacterial contamination on poultry carcasses, as determined by counts of aerobic bacteria, Enterobacteriaceae, and Escherichia coli. Salmonella was not enumerated; rather, it was determined to be present or absent by culturing almost the entire rinse. The prevalence of Salmonella in the study decreased during evisceration, then increased during immersion chilling.     

A study of the effects of Marek's disease in a broiler flock

In a flock of 17000 broilers, characteristic Marek's disease (MD) paralysis, accompanied by increased mortality occurred during the fourth week of the flock's life. Cytolytic lesions and atrophy in the bursae of Fabricius, and the severity of lymphocytic infiltration of nerves, livers, proventriculus and brain are suggestive of the involvement of MD virus of greater pathogenicity than has been encountered previously in New Zealand.     

Reporting temporal fluctuations of hepatic C16 and C18 fatty acids during late gestation and early lactation in dromedary camel

Tropical Animal Health and Production - Based on current knowledge, C16 and C18 fatty acids (FA) are considered the most functional FA in hepatic metabolism. Although these FAs have been...