辽宁省绿色食品发展现状、存在问题及对策

对辽宁省绿色食品发展现状及存在的问题进行了介绍与探讨.分析了绿色食品在农业和食品加工业中的重要地位.同时根据辽宁省实际情况提出了发展绿色食品的相关对策.     

Identification of p53 gene mutations in bladder cancers and urine samples

Although bladder cancers are very common, little is known about their molecular pathogenesis. In this study, invasive bladder cancers were evaluated for the presence of gene mutations in the p53 suppressor gene. Of 18 tumors evaluated, 11 (61 percent) were found to have genetic alterations of p53. The alterations included ten point mutations resulting in single amino acid substitutions, and one 24-base pair deletion. In all but one case, the mutations were associated with chromosome 17p allelic deletions, leaving the cells with only mutant forms of the p53 gene products. Through the use of the polymerase chain reaction and oligomer-specific hybridization, p53 mutations were identified in 1 to 7 percent of the cells within the urine sediment of each of three patients tested. The p53 mutations are the first genetic alterations demonstrated to occur in a high proportion of primary invasive bladder cancers. Detection of such mutations ex vivo has clinical implications for monitoring individuals whose tumor cells are shed extracorporeally.     

Modeling the nucleus and jets of comet 81P/Wild 2 based on the Stardust encounter data

We interpret the nucleus properties and jet activity from the Stardust spacecraft imaging and the onboard dust monitoring system data. Triangulation of 20 jets shows that 2 emanate from the nucleus dark side and 16 emanate from sources that are on slopes where the Sun's elevation is greater than predicted from the fitted triaxial ellipsoid. Seven sources, including five in the Mayo depression, coincide with relatively bright surface spots. Fitting the imaged jets, the spikelike temporal distribution of dust impacts indicates that the spacecraft crossed thin, densely populated sheets of particulate ejecta extending from small sources on the rotating nucleus, consistent with an emission cone model.     

Shape-Controlled Synthesis of Colloidal Platinum Nanoparticles

The shapes and sizes of platinum nanoparticles were controlled by changes in the ratio of the concentration of the capping polymer material to the concentration of the platinum cations used in the reductive synthesis of colloidal particles in solution at room temperature. Tetrahedral, cubic, irregular-prismatic, icosahedral, and cubo-octahedral particle shapes were observed, whose distribution was dependent on the concentration ratio of the capping polymer material to the platinum cation. Controlling the shape of platinum nanoparticles is potentially important in the field of catalysis.     

Comparative metagenomics of microbial communities

The species complexity of microbial communities and challenges in culturing representative isolates make it difficult to obtain assembled genomes. Here we characterize and compare the metabolic capabilities of terrestrial and marine microbial communities using largely unassembled sequence data obtained by shotgun sequencing DNA isolated from the various environments. Quantitative gene content analysis reveals habitat-specific fingerprints that reflect known characteristics of the sampled environments. The identification of environment-specific genes through a gene-centric comparative analysis presents new opportunities for interpreting and diagnosing environments.     

Subsoil rhizosphere modification by chickpea under a dry topsoil: implications for phosphorus acquisition

Chickpea (Cicer arietinum L.) roots exude carboxylates. While chickpea commonly grows where the topsoil dries out during crop growth, the importance of carboxylate exudation by the roots and mobilization of soil P from below the dry topsoil has not been examined. The study investigates the response of carboxylate exudation and soil P mobilization by this crop to subsoil P fertilizer rate. In constructed soil columns in the glasshouse, the P levels (high, low, and nil P) were varied in the well‐watered subsoil (10–30 cm), while a low level of P in the dry topsoil (0–10 cm) was maintained. At flowering, rhizosphere carboxylates and rhizosphere soil from topsoil and subsoil roots were collected separately and analyzed. The concentration of total carboxylates per unit rhizosphere mass in the subsoil was nearly double that of the topsoil. Plants depleted sparingly soluble inorganic P (P_i), NaOH‐P_i, and HCl‐P_i, along with the labile P_i (water soluble and NaHCO₃‐Pi). The P depletion by plants was greater from the subsoil than the topsoil. The study concluded that depletion of sparingly soluble P from the chickpea rhizosphere in the subsoil was linked with the greater levels of carboxylates in the rhizosphere. These findings indicate that chickpea, with its deep rooting pattern, can increase its access to subsoil P when the topsoil dries out during crop growth by subsoil rhizosphere modification.     

Tuber yield, storability, and quality of Michigan cultivars in response to nitrogen management and seedpiece spacing

Information is required on nitrogen (N) fertility and seedpiece management for new cultivars and advanced breeding lines. Interactions amongst N fertilizer rate, genotype, and seedpiece spacing are complex, and can affect tuber yield, quality, and storability as well as N fertilizer efficiency. A field study was carried out in 2001 and 2002 at MSU Montcalm Research Farm in central Michigan. Tuber yields and post-harvest quality characteristics were evaluated for five potato genotypes (MSG227-2, MSE192-8Rus, Jacqueline Lee, Liberator, and Snowden) in response to a factorial combination of three N levels (200 kg N ha^-1, 300 kg N ha^-1-, and 400 kg N ha^-1) and two seedpiece spacings, narrow (0.20 m or 0.25 m) and wide (0.33 m or 0.38 m). Narrow seedpiece spacing consistently produced the highest U.S. No. 1 yields in all genotypes tested (37 and 34 t ha^-1, narrow vs wide spacing, respectively). There was a tradeoff between seedpiece spacing and N level in 2001 as tuber yields were enhanced by higher N levels at wide seedpiece spacing, but not at narrow spacing. In 2002, tuber yield was not enhanced, but petiole nitrate-N and tuber-N increased as N fertilization increased. Genotype was the major factor that influenced tuber quality characteristics at harvest and for stored tubers (e.g., specific gravity, internal defects, bruising, chip color rating, sucrose, and glucose). Spacing had minimal effects, whereas higher levels of N slightly reduced specific gravity both years, reduced internal defects in 2001 and enhanced sucrose at harvest in 2002. The cultivars tested demonstrated excellent storage characteristics for different N fertility levels and seedpiece spacing combinations. Overall, the recommended N fertilizer level for moderately long-duration potato cultivars in Michigan (200 kg N ha^-1) and a narrow seedpiece spacing optimized yield and tuber quality performance while conserving N fertilizer.     

New tetromycin derivatives with anti-trypanosomal and protease inhibitory activities

Four new tetromycin derivatives, tetromycins 1-4 and a previously known one, tetromycin B (5) were isolated from Streptomyces axinellae Pol001(T) cultivated from the Mediterranean sponge Axinella polypoides. Structures were assigned using extensive 1D and 2D NMR spectroscopy as well as HRESIMS analysis. The compounds were tested for antiparasitic activities against Leishmania major and Trypanosoma brucei, and for protease inhibition against several cysteine proteases such as falcipain, rhodesain, cathepsin L, cathepsin B, and viral proteases SARS-CoV M(pro), and PL(pro). The compounds showed antiparasitic activities against T. brucei and time-dependent inhibition of cathepsin L-like proteases with K(i) values in the low micromolar range.