Stabilizing Lead-Salt Diode Lasers: Understanding and Controlling Chaotic Frequency Emission

Lead-salt tunable diode lasers (TDLs) are the only devices currently available that can generate tunable monochromatic radiation at arbitrary wavelengths between 3 and 30 micrometers and are particularly useful for high-resolution spectroscopy over a wide range of spectral regimes. Detailed observations of TDLs show that the observed instrumental linewidth is actually a temporal average of many narrow (less than 0.5 megahertz) emission "modes." The time scale characteristic of these "modes," which appear to be of relatively constant intensity, is of the order of a microsecond. The laser's behavior is highly suggestive of a chaotic process, that is, seemingly random excursions of a dynamic variable (frequency) within a bounded range. This report shows experimentally that TDL emissions are indeed chaotic. Furthermore, in a simple and robust fashion, this chaotic behavior has been successfully controlled with the use of recent techniques that take advantage of chaos to produce a narrow band laser output.     

Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro

Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.     

土地沙漠化过程的土壤风蚀率指标——以青海共和盆地为例

由风蚀所导致的土壤物质再分配过程,决定了土地沙漠化的程度。根据半干旱干草原地区不同程度沙漠化土地土壤风蚀率的137Cs示踪结果,沙漠化各个阶段的土壤风蚀率指标为:潜在沙漠化土地以微弱堆积和微度风蚀为特征,风蚀率小于2000kg/(hm2·a);轻度沙漠化土地风蚀率介于2000～7000kg/(hm2·a)之间;中度沙漠化土地风蚀率大于7000kg/(hm2·a);重度沙漠化土地风蚀和堆积都很强烈,地表物质交换迅速,没有必要制定风蚀指标。     

不同气候对氧化型降解地膜的影响（英文）

【目的】通过在三个不同气候地区的试验,研究气候因素对农用氧化型降解地膜降解的影响,为此类降解地膜在不同地区的应用而调整添加剂配方提供指导。【方法】太阳辐射、温度和湿度是控制降解速度的主要因素,通过红外光谱分析,力学性能和分子量测试来评估他们对降解的影响。【结果】太阳辐射强度对氧化型降解地膜的降解影响最大。经过6个月太阳照射后,含有高浓度添加剂的降解膜,降解速率快。【结论】氧化型农用降解地膜的降解速率主要受太阳辐射强度控制,温度和湿度对降解速率影响较轻。当分子量小于5道尔顿时,降解膜碎片能够被微生物消化。     

Immunodominant antigens of zoospores from ovine isolates of Dermatophilus congolensis.

Zoospores of Dermatophilus congolensis were analysed by SDS-PAGE and western blotting. The electrophoretic profiles of zoospores from 13 isolates of D. congolensis were similar but not identical when stained with Coomassie blue or silver. Immunodominant polypeptides with apparent molecular masses of 76 and 31 kDa were identified in western blots of 13 of 13 and 12 of 13 isolates respectively of D. congolensis reacted with hyperimmune, ovine, antizoospore sera. Identical immunodominant polypeptides were observed in western blots reacted with sera obtained from naturally infected sheep. Initial characterisation of the 76 and 31 kDa polypeptides indicated that they were probably surface exposed because (i) antibodies eluted from the surface of live zoospores after adsorption of hyperimmune antizoospore serum, reacted principally against the 76 and 31 kDa subunit polypeptides in western blots, (ii) adsorption of hyperimmune antizoospore serum with live zoospores resulted in significant diminution of reactivity against both the 76 and 31 kDa polypeptides in western blots, (iii) indirect fluorescent immunostaining of zoospores with antiserum prepared against gel-purified 76 kDa polypeptide, resulted in intense staining of the zoospore outer coat. Immuno-gold electron microscopy of negatively stained zoospores with antiserum prepared against gel-purified 31 kDa polypeptide identified this antigen as a flagella subunit.     

Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries

OBJECTIVE: To identify virulence genes in enterotoxigenic E coli (ETEC) isolates associated with diarrhoea in neonatal, 1 to 3 week-old and weaned pigs in southeast Queensland. DESIGN: Multiplex PCR and serotyping were applied to E coli isolates obtained over a 5-year period (1998-2002) from cases diagnosed at Toowoomba Veterinary Laboratory. PROCEDURE: A total of 126 isolates from 25 different Queensland piggeries were tested for haemolytic activity on 5% sheep blood agar and by multiplex PCR for the presence of five commonly recognised fimbrial (F4, F5, F6, F41 and F18) and three enterotoxin genes (STa, STb, LT). A subset of 62 representative isolates were serotyped by slide agglutination. For comparative purposes, multiplex PCR was also performed on the DNA of 31 ETEC isolates from 9 serotypes originating from piggeries in southern New South Wales. RESULTS: A total of 113 (89.7%) of the isolates from Queensland possessed ETEC virulence genes, including 14 of 15 isolates from neonatal pigs (93.3%), 18 of 23 isolates from 1 to 3 week old pigs (78.3%) and 81 of 88 isolates from weaned pigs (92.1%). F4:STa:STb:LT (serotype O149) was the most prevalent pathotype in neonatal and 1-3 week old pigs and F4:STa:STb:LT (serotype O149) and F18:STa:STb:LT (serotype O141) were most prevalent in weaned pigs. In comparison, isolates obtained from neonatal pigs from New South Wales belonged to a more diverse range of pathotypes and serotypes. CONCLUSION: Multiplex PCR was a rapid and specific method for detecting the presence of ETEC virulence genes in porcine E coli isolates. For isolates obtained from cases of suspected colibacillosis in Queensland, growth of a heavy pure culture of haemolytic E coli was a sensitive prognostic indicator of the presence of ETEC virulence genes in the isolate. ETEC pathotypes and serotypes remained stable in Queensland piggeries over the five-year study period and appear to have changed little over the last three decades.     

Phenotypic indicators for cultured pearl size improvement in the black‐lipped pearl oyster (Pinctada margaritifera): towards selection for the recipient growth performance

The black‐lipped pearl oyster, Pinctada margaritifera, is the most important farmed species in French Polynesia and the basis of the most valuable export industry. Mass production of black pearls relies on a surgical operation requiring tissue from a donor pearl oyster to be grafted, together with a nucleus made of shell, into the gonad of a recipient oyster. Improving pearl size through family selection remains one of the main challenges for future aquaculture development. This study analyses the relative contribution of donor and recipient oysters to pearl size. To this end, hatchery‐produced donor oysters of two batches, large and small (based on shell height), were used to supply grafts for recipients, which were then monitored individually for their growth performance by recording shell height, width, and thickness, and total live weight (flesh + shells) every 6 months (four biometric measurement times) over 20 months of culture. Pearls issued from the two batches of donors showed no significant differences in nacre weight or thickness. In contrast, recipient oyster shell height and total weight were increasingly positively correlated with these pearl size parameters over the culture period, becoming significant at 8 months post‐grafting. Potential therefore exists to use shell height and oyster weight as phenotypic indicators for selective breeding of recipient oysters with high growth performance to increase pearl size in P. margaritifera.     

白斑狗鱼耗氧率和窒息点研究

白斑狗鱼(Esoxlucius)属鲑形目(Salmoniformes)、狗鱼科(Esocidae)、狗鱼属(Esox),北半球北部亚冷水性大型凶猛淡水鱼类,我国仅产于新疆北部额尔齐斯河流域,如额尔齐斯河、布尔津河、哈巴河等及附属水体。鱼类大多数代谢活动都和氧的利用有关,耗氧率直接或间接地反映新陈代     

四种滤料去除氨氮的效果

测定生化环、沸石、生化石和碎石4种滤料的去除氨氮效果。结果显示,单位体积滤料去氨氮效果以沸石最佳[4.19mg/(h·L)],生化环和生化石去氨氮能力基本相同,两者分别为3.60mg/(h·L)与3.58mg/(h·L),效果最差的碎石仅为2.09mg/(h·L);若以单位质量滤料表示其去氨氮能力,则不同滤料的去氨氮能力差异显著,其中生化环去氨氮能力最强[5.29mg/(h·kg)],其次为生化石,其去氨氮能力为4.07mg/(h·kg),沸石的去氨氮能力较差[1.95mg/(h·kg)],仅为生化环的37.0%,碎石的去氨氮能力最差[0.87mg/(h·kg)],为生化环的16.4%。