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51.
Effect of Leukemia Inhibitory Factor and Forskolin on Establishment of Rat
Embryonic Stem Cell Lines
Masumi HIRABAYASHI Teppei GOTO Chihiro TAMURA Makoto SANBO Hiromasa HARA Shinichi HOCHI 《The Journal of reproduction and development》2014,60(1):78-82
This study was designed to investigate whether supplementation of 2i medium with
leukemia inhibitory factor (LIF) and/or forskolin would support establishment of
germline-competent rat embryonic stem (ES) cell lines. Due to the higher likelihood of
outgrowth rates, supplementation of forskolin with or without LIF contributed to the
higher establishment efficiency of ES cell lines in the WDB strain. Germline transmission
competency of the chimeric rats was not influenced by the profile of ES cell lines until
their establishment. When the LIF/forskolin-supplemented 2i medium was used, the rat
strain used as the blastocyst donor, such as the WI strain, was a possible factor
negatively influencing the establishment efficiency of ES cell lines. Once ES cell lines
were established, all lines were found to be germline-competent by a progeny test in
chimeric rats. In conclusion, both LIF and forskolin are not essential but can play a
beneficial role in the establishment of “genuine” rat ES cell lines. 相似文献
52.
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54.
Mohammad M. Monir Kohzy Hiramatsu Sachiko Matsumoto Kei Nishimura Chihiro Takemoto Tomomi Shioji Takafumi Watanabe Kazumi Kita Shinichi Yonekura Sang Gun Roh 《Animal Science Journal》2014,85(5):581-587
Influences of a specific dietary nutrient on glucagon‐like peptide (GLP)‐1‐containing cells in the chicken intestine are not yet clear. Significance of dietary protein level on GLP‐1‐containing cells in the chicken ileum was investigated. Chickens fed control or experimental diets of varying protein levels were examined using immunohistochemical and morphometrical techniques. We show that the protein ingestion had an impact on the activities of GLP‐1‐immunoreactive cells in the chicken ileum. Weight gains declined with decreasing dietary crude protein (CP) levels, but no significant differences were detected in the daily feed intake and villous height. GLP‐1‐immunoreactive cells with a round or oval shape were frequently observed in the lower CP level groups (4.5% and 0%). Frequencies of occurrence of GLP‐1‐immunoreactive cells were 41.1 ± 4.1, 38.5 ± 4, 34.8 ± 3.1 and 34.3 ± 3.7 (cells/mm2, mean ± SD) for dietary CP level of 18%, 9%, 4.5% and 0% groups, respectively and significant differences were recognized between the control and lower CP level groups (P < 0.05). Multiple regression analysis indicated a significant correlation between the daily protein intake and frequencies of occurrence of GLP‐1‐immunoreactive cells. The protein ingestion is one of the signals that influence GLP‐1‐containing cells in the chicken small intestine. 相似文献
55.
Polyadenylic acid in Visna virus RNA 总被引:13,自引:0,他引:13
Visna virus 70S RNA contains long stretches of polyadenylic acid [poly(A)]. The homogeneity in length of poly(4) regions is observed in 70S RNA from visna virus and all RNA tumor viruses tested, and not with other types of RNA. By this criterion visna virus resembles RNA tumor viruses. 相似文献
56.
Hamauzu Y Yasui H Inno T Kume C Omanyuda M 《Journal of agricultural and food chemistry》2005,53(4):928-934
To evaluate the phenolic extracts of Chinese quince, quince, and apple fruits, their phenolic profiles, antioxidant properties, and anti-influenza viral activities were investigated. Chinese quince had the largest amount of phenolics consisting mainly of high polymeric procyanidins. Quince had considerable amounts of hydroxycinnamic derivatives mainly composed of 3-caffeoylquinic acid and 5-caffeoylquinic acid and polymeric procyanidins. Apple (cv. Fuji) had the lowest amount of phenolics, mainly 5-caffeoylquinic acid and monomeric and oligomeric procyanidins. The antioxidant functions of Chinese quince and quince phenolic extracts were superior to that of chlorogenic acid standard or ascorbic acid evaluated in both the linoleic acid peroxidation system and the DPPH radical scavenging system. However, those extracts were less effective than apple phenolics or (-)-epicatechin in linoleic acid peroxidation system. On the other hand, Chinese quince phenolics showed the strongest anti-influenza viral activity on the hemagglutination inhibition test. 相似文献
57.
Shuhei Takemoto Hitoshi Nakamura Atsuko Sasaki Takanori Shimane 《Journal of General Plant Pathology》2011,77(2):107-111
Rosellinia compacta, described recently, resembles R. necatrix and also causes white root rot. Here a species-specific PCR was developed for R. compacta, and the two R. necatrix-specific primer sets already available were validated in terms of species specificity. PCRs using the primer sets for R. necatrix amplified specific products exclusively from R. necatrix isolates. The R. compacta-specific primer set exclusively detected R. compacta, which appears to be a rare but widely distributed species. We conclude that R. necatrix is the major cause of the disease in Japan but that the involvement of R. compacta should be studied further. 相似文献
58.
Kido N Wada Y Takahashi M Kamegaya C Omiya T Yamamoto Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(6):845-847
The prevalence of Dirofilaria immitis in free-ranging raccoon dogs (Nyctereutes procyonoides viverrinus) was examined in the southeast region of Kanagawa Prefecture, Japan, using a rapid immunomigration (RIM) test kit. Between April 2007 and March 2010, we examined 108 raccoon dogs rescued and housed by the Kanazawa Zoological Garden. D. immitis infection was found in 8 (7.4%) raccoon dogs. This is the first report to reveal the prevalence of D. immitis infection in living raccoon dogs. The prevalence of the infection was lower than previously reported values obtained on postmortem examination. One reason might be that the present study included young raccoon dogs infected with immature worms. Significant high-risk areas of D. immitis infection in the raccoon dogs were not observed. 相似文献
59.
To predict soil moisture condition in arbitral agricultural lands by taking spatial distribution of soil type in a watershed into account, we investigated the possible proper methods of determining the soil hydrological parameters using available soil physical properties database of Japanese soils, “SolphyJ”, and the digital soil map. In this study, simulation of soil moisture was conducted at three neighboring locations, including monitoring sites each in Toyama and Fukui cities. The simulated results of soil moisture appeared to be improved when hydraulic parameters were obtained by fitting water retention data of SolphyJ compared to the parameters estimated by neural network with soil texture and dry bulk density data. It is probably because peculiar soil structure in each field could affect the hydraulic properties more than the soil texture. Simulation results also indicated that soil moistures are much different even if they are located in neighbors (<5 km). We concluded that combination of these two databases is useful for estimating soil hydraulic parameters and to predict soil moisture condition in arbitrary agricultural lands. 相似文献
60.
Tropical Animal Health and Production - 相似文献