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排序方式: 共有109条查询结果,搜索用时 15 毫秒
31.
Ohba Y Takasu M Nishii N Takeda E Maeda S Kunieda T Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):297-299
Using a DNA-based diagnostic test for factor XI deficiency in Japanese black cattle, we surveyed 123 cattle (42 sires and 81 dams) in Gifu and Hyogo prefectures, and calculated gene frequencies. In sires, we drew up the pedigree network of the cattle with the factor XI deficiency. Results showed that the mutated allele of factor XI deficiency was retroactive in at least 6 or more generations of sires. Frequencies of the mutant gene were higher at 26.4% in total, and at 33.3% in sires. All 7 cattle with the homozygote of mutated allele were clinically normal, and showed no bleeding episodes. The mutated allele of factor XI deficiency might be widespread among Japanese black cattle. 相似文献
32.
Masuda K Hashizume C Kikusui T Takeuchi Y Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(2):183-187
Catechol O-methyltransferase (COMT) inactivates catecholamines and catechol-containing drugs such as L-DOPA. The common genetic polymorphism Val158Met in the human COMT gene is suspected to be associated with "persistence" or risk for schizophrenia. In this study, we attempted to identify the canine COMT gene fragment and to find a similar polymorphism and to reveal its genetic distribution among five representative canine breeds. We found that the amplified gene consisted of 663 bp nucleotides and was 84% homologous with the human COMT gene. The single nucleotide polymorphisms, guanine adenine substitution, were observed at the 39th, 216th and 482nd nucleotides. From the genotyping of the 216th polymorphism among 266 dogs by the polymerase chain reaction-restriction fragment length polymorphism method with restriction enzyme EagI, and that of the 482nd polymorphism with restriction enzyme SfcI, we found inter-breed variations of genotypes as well as of allelic frequencies for both of these polymorphic regions. These results suggest that the identified polymorphisms will be useful tools in elucidating the genetic background of canine behavioral traits. 相似文献
33.
34.
Shogo HIGAKI Hongyu DARHAN Chie SUZUKI Tomoko SUDA Reina SAKURAI Koji YOSHIOKA 《The Journal of reproduction and development》2021,67(1):67
We aimed to determine the effectiveness of estrus detection based on continuous measurements of the ventral tail base surface temperature (ST) with supervised machine learning in cattle. ST data were obtained through 51 estrus cycles on 11 female cattle (six Holsteins and five Japanese Blacks) using the tail-attached sensor. Three estrus detection models were constructed with the training data (n = 17) using machine learning techniques (random forest, artificial neural network, and support vector machine) based on 13 features extracted from sensing data (indicative of estrus-associated ST changes). Estrus detection abilities of the three models on test data (n = 34) were not statistically different among models in terms of sensitivity and precision (range 50.0% to 58.8% and 60.6% to 73.1%, respectively). The relatively poor performance of the models might indicate the difficulty of separating estrus-associated ST changes from estrus-independent fluctuations in ST. 相似文献
35.
Higaki-Sato N Sato K Esumi Y Okumura T Yoshikawa H Tanaka-Kuwajima C Kurata A Kotaru M Kawabata M Nakamura Y Ohtsuki K 《Journal of agricultural and food chemistry》2003,51(1):8-13
An enzymatic hydrolysate of wheat gluten was further digested in vitro with porcine pepsin and pancreatin to obtain an indigestible peptide. Indigestible pyroglutamyl peptide was isolated from the digest by strong cation-exchange, size-exclusion, and reversed-phase chromatographies. The pyroglutamyl peptide was digested with pyroglutamate aminopeptidase, and the digest was reacted with phenyl isothiocyanate. The resultant phenylthiocarbamyl (PTC) peptides were purified by reversed-phase HPLC by using binary gradient elution with ammonium acetate buffer, pH 6.0, and acetonitrile. The PTC peptides were analyzed with an automatic peptide sequencer on the basis of the Edman degradation method with a modified program. Some pyroglutamyl peptides were also analyzed by fast-atom bombardment ionization mass spectrometry without the pyroglutamate amino peptidase digestion. Consequently, pyroGlu-Asn-Pro-Gln, pyroGlu-Gln-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gly-Gln-Gly-Gln, pyroGlu-Gln, pyroGlu-Gln-Pro, pyroGlu-Ile-Pro-Gln, pyroGlu-Ile-Pro, pyroGlu-Gln-Pro-Leu, pyroGlu-Gln-Phe-Pro-Gln, pyroGlu-Ser-Phe-Pro-Gln, pyroGlu-Phe-Pro-Gln, and pyroGlu-Gln-Pro-Pro-Phe-Ser were identified. 相似文献
36.
37.
Hafez E Takahashi T Ogawa H Sato M Nakai T Takasu C Uehara H Izumi K 《Journal of toxicologic pathology》2011,24(4):187-193
Diabetes mellitus (DM) and obesity are believed to be risk factors for colorectal cancer
in humans. In experiment 1, male nondiabetic Long-Evans Tokushima Otsuka (LETO) rats and
Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model animal of type 2 DM, were
whole-body X-irradiated (4 Gy) at 6 and 8 weeks of age and euthanized at 78 weeks of age
(n=15, respectively). The incidences of small intestine adenocarcinoma in LETO and OLETF
rats were 0% and 30%, respectively. In experiment 2, male LETO and OLETF rats (n=24,
respectively) were given s.c. injections of 15 mg/kg azoxymethane (AOM) once weekly for 3
weeks and euthanized at 36 weeks of age. The incidences of Zymbal gland tumors in LETO and
OLETF rats were 0% and 67%, respectively (P<0.001), whereas those of
small intestine adenocarcinoma were 0% and 43% (P<0.001) and those of
cecum/colon adenocarcinoma were 46% and 79% (P<0.05), respectively.
Fatty change of hepatocytes was common in OLETF rats (63%) but not in LETO rats. Serum
triglyceride and free fatty acid levels in OLETF rats were significantly higher than in
LETO rats at sacrifice, whereas serum insulin levels in OLETF rats were very diverse.
These data suggest that hyperlipidemia plays a significant role in high susceptibility to
lower intestinal tract carcinogenesis in OLETF rats; this strain is susceptible to
AOM-induced Zymbal gland carcinogenesis. 相似文献
38.
Ando M Kado S Suzuki T Yamamoto R Nagata Y Hata C Uchida K Kaneko K 《Journal of toxicologic pathology》2011,24(4):251-255
A subcutaneous tumor was found in the right abdomen of a 16-week-old male TCRβ and p53 double-knockout mouse. The tumor had indistinct borders with the surrounding tissue. The cut surface after formalin fixation was pale yellowish white, partially dark red and partly white. Histologically, the tumor was composed of three distinct regions. The first region showed pleomorphic cells arranged in sheets. The second region showed spindle cells arranged in interlacing fascicles. The final region contained a mixture of the above mentioned two types of cells. Furthermore, a small amount of collagen fibers, round cells, multinucleated giant cells, and cells with eosinophilic granules were observed between these tumor cells. Immunohistochemical examination and electron microscopy identified that the pleomorphic cells and spindle cells were histiocytes and fibroblasts, respectively, and that the round cells were undifferentiated mesenchymal cells. Based on these findings, the tumor was diagnosed as a malignant fibrous histiocytoma. 相似文献
39.
Yamagishi C Momoi Y Kobayashi T Ide K Ohno K Tsujimoto H Iwasaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):915-924
In this study, a retrospective analysis was conducted to assess the current aspects and predisposing factors of canine sterile panniculitis. Miniature dachshund, neutered, and younger dogs appeared to be predisposed. In addition, histories of previous surgery and injection were associated in 46.5% of the cases, with several types of surgical suture materials used. About 88% of the dogs had multifocal lesions, frequently with signs of systemic illnesses. Whereas systemic immunosuppressive therapy was effective in most dogs, surgical excision of lesions was rarely curative. In order to prevent recurrences, over 65% of the cases required prolonged immunosuppressive therapy. Polymorphism of canine alpha(1)AT gene was investigated as a candidate gene for sterile panniculitis. Eight polymorphisms were discovered in seven Miniature dachshunds by direct nucleotide sequencing, which included a 12-bp deletion, three non-synonymous single nucleotide polymorphisms, and four silent substitutions. Genotyping of the two polymorphisms, c.109_120del12 and c.483A>C, which identified at high incidence in the dachshunds, was conducted in 83 dogs of 6 popular breeds. The frequencies of neither polymorphism differed between Miniature dachshunds and other breeds, suggesting that neither is responsible for developing panniculitis. 相似文献
40.
Chie Hayakawa Kazumichi Fujii Shinya Funakawa Takashi Kosaki 《Soil Science and Plant Nutrition》2013,59(5):663-673
Low molecular weight (LMW) organic compounds in soil solution are easily biodegradable and could fuel respiration by soil microorganisms. Our main aim was to study the mineralization kinetics of monosaccharides using 14C-radiolabelled glucose. Based on these data and the soil solution concentrations of monosaccharides, we evaluated the contribution of monosaccharides to basal respiration for a variety of tropical forest soils. Further, the factors controlling the mineralization kinetics of monosaccharides were examined by comparing tropical and temperate forest soils. Monosaccharides comprised on average 5.2 to 47.7% of dissolved organic carbon in soil solution. Their kinetic parameters (V max and KM ), which were described by a single Michaelis-Menten equation, varied widely from 11 to 152?nmol?g?1?h?1 and 198 to 1294?µmol?L?1 for tropical soils, and from 182 to 400?nmol?g?1?h?1 and 1277 to 3150?µmol?L?1 for temperate soils, respectively. The values of V max increased with increasing microbial biomass-C in tropical and temperate soils, while the KM values had no correlations with soil biological or physicochemical properties. The positive correlation between V max values and microbial biomass-C indicates that microbial biomass-C is an essential factor to regulate the V max values in tropical and temperate forest soils. The biodegradation kinetics of monosaccharides indicate that the microbial capacity of monosaccharide mineralization far exceeds its rate at soil solution concentration. Monosaccharides in soil solution are rapidly mineralized, and their mean residence times in this study were very short (0.4–1.9?h) in tropical forests. The rates of monosaccharide mineralization at actual soil solution concentrations made up 22–118% of basal respiration. Probably because of the rapid and continuous production and consumption of monosaccharides, monosaccharide mineralization is shown to be a dominant fraction of basal respiration in tropical forest soils, as well as in temperate and boreal forest soils. 相似文献