Microbiological properties of Myanmar traditional shrimp sauce,hmyin-ngan-pya-ye

Fisheries Science - The microbiological properties of Myanmar traditional fermented products were characterized using four different brands of bottled shrimp sauce products sold frequently at a...     

Establishment of a model for chemoattraction of <Emphasis Type="Italic">Symbiodinium</Emphasis> and characterization of chemotactic compounds in <Emphasis Type="Italic">Acropora tenuis</Emphasis>

Corals harbor symbiotic dinoflagellates, Symbiodinium spp., acquired from surrounding environments. Because Symbiodinium are present at low densities in the water column, corals may attract these symbionts using chemotactic compounds. To examine whether corals contain chemotactic compounds, we established an assay to measure the chemotactic activity for Symbiodinium using an extract of the coral Acropora tenuis, a major reef-building coral in Japan. Our assay revealed that Symbiodinium strain NBRC102920 (clade A), which is taken up by juvenile A. tenuis polyps, is attracted to crude A. tenuis extracts. We found that the chemotactic compounds are water-soluble, heat-labile macromolecules and that the chemotactic activity was inhibited by N-acetyl-d-glucosamine (GlcNAc). We separated the GlcNAc-binding fraction (Fr-ActL) and identified it as the most plausible candidate for the chemoattractant, since the chemotactic activity of the crude A. tenuis extract appeared to be mainly attributable to the activity of Fr-ActL and was also inhibited by the addition of GlcNAc. These results indicate that chemoattraction is mediated via the binding of Symbiodinium to Fr-ActL.     

Relationships between quality parameters and content of glycerol galactoside and porphyra-334 in dried laver nori <Emphasis Type="Italic">Porphyra yezoensis</Emphasis>

ABSTRACT:   Glycerol galactoside (GG; floridoside + isofloridoside) and porphyra-334 (P-334) are contained in nori (Susabinori Porphyra yezoensis and Asakusanori Porphyra tenera ). Glycerol galactoside has been found to have bifidogenic growth stimulator activity and P-334 is known to have ultraviolet-absorbing activity in the UVA region of sunlight. These substances have, respectively, potential for application to pre-biotic foods and in cosmetics as a sunscreen. In the present study, to investigate the relationships between GG and P-334 contents and the quality of nori, we measured the GG and P-334 contents with other components (total protein, chlorophyll-a, β-carotene and phycobillins) that are related to the quality of nori samples produced from different production areas and with different qualities. We found that the GG content was closely negatively correlated with the contents of other components, whereas P-334 was positively correlated with the other components. From these results, it is suggested that low-quality nori is a potential source of GG, and as a source for P-334, scraps of nori produced during nori processing should be suitable.     

Improved method of protoplast fusion between marine luminescent bacterium and <Emphasis Type="Italic">Escherichia coli</Emphasis> cultivated with fatty acid

An attempt was made to improve the stability toward centrifugation of protoplast fusion between Shewanella sp. and Escherichia coli. Stability of the cell membrane is an important factor in protoplast fusion. In order to change the fatty acid composition of the cell membrane phospholipids, eight fatty acids [caprylic acid, capric acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, eicosapentaenoic acid (EPA) and docosahexaenoic acid] were added to each nutrient medium of Shewanella sp. and E. coli. The protoplasts were treated with lysozyme, and fusion occurred in the presence of a polyethylene glycol solution. The stability of the protoplast of Shewanella sp. decreased after EPA was added to the culture medium, and the stability of the protoplast of E. coli increased after the addition of linoleic acid or linolenic acid. Some fusant colonies that developed on the regenerated medium selected for E. coli with antibiotic tolerance. The efficiency of this fusion was higher than that of initial condition using protoplasts from Shewanella sp. and E. coli incubated without fatty acids. Protoplasts improved the fatty acid composition of the phospholipids. Cell membrane stability can change in order for the weak cells to be taken in by strong cells. These results suggested that the fatty acid composition of cell membrane phospholipids affected the fusant yield of the fusion of these bacteria.     

Factors influencing steel tool wear when milling wood

The wear of high speed steel cutting tools after milling wood of four selected wood species was studied. For the experiments wood specimens were chosen with very different silica contamination, wood density, and high temperature corrosivity (HTC) of wood towards tool material. Analyses performed show that the silica content and wood density display poor linear correlation with the cutting tools wear, while the correlation of the HTC appeared very good. The silica contamination and the HTC effects overshadow each other. Thus, a theoretical multivariable simulation of the cutting edge wearing process, employing all experimental variables, was applied, providing a very good explanation of the analyzed problem.     

Performance of coated carbide tools in turning wood-based materials: Effect of cutting speeds and coating materials on the wear characteristics of coated carbide tools in turning wood-chip cement board

This paper reports on the performance of coated carbide tools in turning wood-chip cement board. The coating materials studied were titanium carbonitride (TiCN), titanium nitride (TiN), chromium nitride (CrN), and titanium nitride/aluminum nitride (TiN/AlN), which were synthesized on the P30 carbide substrate. The aim is to investigate the effect of coating materials and cutting speeds on the wear characteristics of the coated carbide tools. Cutting tests were performed when turning wood-chip cement board at cutting speeds of 30, 40, 50 and 60 m/s, a depth of cut of 1mm, and a feed of 0.05 mm/rev. The results of the study show that the coated carbide tools provided better performance than the P30 carbide tool, especially in terms of reducing the progression of the wear rate and clearance wear. The TiN/AlN-coated carbide tool showed the smallest increase in both wear rate and clearance wear with an increase in cutting speed and had the longest tool life among the coated carbide tools investigated. Though the TiCN-coated carbide tool was observed to have a low wear rate and low clearance wear for cutting speeds of 30 and 40m/s, when the cutting speeds were >50m/s its wear rate and clearance wear were almost the same as those of CrN-and TiN-coated carbide tools, which had high values for these parameters.Part of this paper was presented at the 21st IUFRO World Congress, August 2000, Kuala Lumpur, Malaysia     

The critical rotational speed of circular saw: simple measurement method and its practical implementations

Operation safety during sawing operations as well as dimensional accuracy and surface roughness depend on circular saw dynamic features among other factors such as circular saw blade accuracy and static/dynamic properties of the machine tool. Manufacturers of saw blades have an obligation to mark tools with a value stating the maximum allowed rotational speed for each saw. However, in some cases the value indicated on the saw corresponds to the critical rotational speed or is dangerously close to this critical value. Saw operation at the critical rotational speed is inadvisable and may result in serious injury or depreciation of product quality. This report outlines a simple methodology for evaluation of circular saw critical rotational speed. The assessment was conducted with a camera vision technique on the basis of an impulse test. Results are compared with theoretically calculated critical rotational speeds and the marks on saw blades.     

Evaluation of surface smoothness using a light-sectioning shadow scanner

A new type of sensor for rapid three-dimensional evaluation of surface geometrical properties is presented. Light emitted with a fixed small angle to the surface plane by a projector is directed into the measured surface. A curtain installed in the light path close to the surface creates a shadow on the measured surface. The shape of the border between bright (highly lit area) and dark (shadow area) is a profile section of the surface. The camera installed over the measured surface captures an image of the border and a digital signal processor using image analysis techniques digitizes the profile section. The shadow scanner method evaluated here could be used for rapid and accurate scans of surfaces of various porous materials, particularly wood, veneer, paper, fiberboards, leaves, and similar materials in both laboratory and industrial applications. The simplicity of the sensor is an advantage because it makes the system easy to maintain, resistant to breakage, and inexpensive. Its straightforward nature and high accuracy enables the method to be utilized for on-line measurement, and therefore it is suitable for industrial application.     

Effect of combinations of wood and counterface materials on three-body abrasive wear

The abrasion characteristics of various combinations of wood and counterface materials in three-body abrasive wear were investigated. Various wood samples were examined in combination with wood, plastic, and metal counterface materials. The wear coefficient in the wood samples was calculated as the wear volume of the friction surface divided by the sliding distance and the applied load. The results showed that the wear coefficient was smaller in cases where the wood samples had greater yield stress. The wear coefficient increased as the yield stress of the various counterface materials increased, reaching a maximum value and then decreased as the yield stress increased. This result indicated that a peak value existed for the wear coefficient in combination with the counterface material.Part of this report was presented at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001