Altered adenosine triphosphatase activities in pigs with naturally occurring hypertrophic cardiomyopathy

The purpose of this study was to determine whether myocardial adenosine triphosphatase (ATPase) activities were reduced in pigs with naturally occurring hypertrophic cardiomyopathy (HCM). The selection of hearts for the HCM and the normal control groups depended on histological examination. Specific ATPase activity and 5-nucleotidase activity were measured in left ventricular myocardium obtained from HCM (n=7) and normal control (n=7) animals. The histological features of HCM included marked disorientation of muscle cells, thickening of the intramural coronary arterial wall with a narrowed lumen, endocardial fibrosis and myocardial fibrosis. The HCM group showed significant increases in both heart weight (32%) and heart weight to body weight ratio (46%). The total ATPase activity in crude homogenates from the HCM group was significantly decreased by 16%. Azide-sensitive ATPase (mitochondrial ATPase) activity, ouabain-sensitive ATPase (Na⁺,K⁺-ATPase) activity, basal Mg²⁺-ATPase activity and Ca²⁺-ATPase activity were all significantly decreased by 18%, 30%, 20% and 50%, respectively. In contrast, no significant decrease was found in the mean values for 5-nucleotidase activity. These results suggest that myocardial ATPase activities are suppressed in pigs with naturally occurring HCMAbbreviations ATP adenosine triphosphate - gww grams wet weight - HCM hypertrophic cardiomyopathy - Pi inorganic phosphate     

西藏的小麦白秆病

小麦白秆病是在青藏高原上为害小麦的一种新病害,在受害的叶、叶鞘及茎秆上产生草黄色条斑和椭圆形至长方形斑点两种症状。经鉴定病害由壳月孢属(Selenophoma)真菌引起。病菌在15℃黑暗条件下的马铃薯葡萄糖琼脂上培养,形成粘质状菌落,生长缓慢,不形成分生孢子器,而在菌丝上芽殖分生孢子;在15℃黑光灯照射下的燕麦琼脂等培养基上培养,形成分生孢子器。分生孢子器分生孢子新月形,无色,单胞,大小为17.9-26×2.6-3.6μ;而芽殖分生孢子的大小及形态变异较大。病菌的生长温度范围为0—20℃,以15℃为最适宜。其寄主有小麦、黑麦及冰草等。种子和幼苗经接种后,均产生系统性条斑症状。根据病菌的形态和寄主范围,小麦白秆病菌即Selenophoma donacis(pass)sprague efA.G.Johnson,由于S.donacis 只产生斑点症状,而小麦白秆病菌产生特有的系统性条斑症状,且发生温度偏低,所以认为小麦白秆病菌是在青藏高原特殊的生境条件下,形成的一个Selenophoma donacis(pass)sprague et A.G.Johnson的“白秆(albo-culmo)生态型。     

The in vivo sensitivity of ATPases to DDT,DDE, and physical immobilization in American cockroaches

American cockroaches injected with sublethal doses of DDT (0.75 μg/roach) at 5-day intervals showed a 40% reduction in oligomycin-sensitive Mg²⁺ATPase from muscle homogenates, and a 23% reduction of Na⁺-K⁺ATPase from nerve cords. Thus, the maximum effect measured occurred with the same enzyme and tissue as determined from in vitro studies. The metabolite, DDE, used at 15 μg per roach, gave no significant change in activity of the ATPase system following injection. In contrast, high single doses of DDT (7.5 μg/roach) and 100 μg DDE and dicofol per roach caused over 30% increase in oligomycin-sensitive Mg²⁺ATPase of muscle and a 10–15% increase in Na⁺-K⁺ATPase of nerve cords measured 24 and 48 hr later. While a similar response was observed for Mg²⁺ATPase activities in cockroaches that were immobilized, the increase in enzyme activities were much greater than that caused by the pesticides.     

草莓杯装软罐头的研制

试验对杯装草莓罐头的工艺进行了研究。结果表明,烫漂的工艺为75 ℃、4 min(分钟);注液汤汁的配比为白砂糖25 %、柠檬酸0.3 %、氯化钙0.2 %;杀菌工艺为7.5 kGy辐射杀菌。     

胚培杏新品种———山农凯新1号

 ‘山农凯新1号’是以丰产性强的‘凯特’杏为母本, 以品质优良的‘新世纪’杏为父本进行有性杂交, 结合胚培技术育成的新品种, 具有成熟早、香味浓、品质优、自交坐果率高、丰产性强等特点。     

不同施肥期沾化冬枣对15N的吸收、分配及利用特性

 盆栽条件下利用¹⁵N示踪技术, 研究不同时期施15N - 尿素, 对沾化冬枣¹⁵N的吸收利用及分配特性的影响。结果表明: 生长季前期(萌芽前和花前) 施用¹⁵N - 尿素, 经根系吸收后, ¹⁵N优先分配到贮藏器官(包括主干、多年生枝和粗根) 中, 然后外运用于树体新生器官(包括枣吊及其叶片、新生营养枝、细根及果实) 的形成, 果实采收后¹⁵N开始向贮藏器官回流; 果实硬核期¹⁵N直接用于树体营养生长和生殖生长, 而不是先贮藏再利用; 果实速长期¹⁵N优先向贮藏器官中积累; 萌芽前施¹⁵N在树体内的运转规律符合落叶果树贮藏N营养分配规律, 优先转运到生长中心。随着施肥期的后延, 植株对¹⁵N - 尿素的当季利用率逐渐下降。     

苹果采前落果与内源激素的关系

为了探讨苹果采前落果与内源激素之间的关系,在采前8周间定期测定了不同苹果品种的果柄、果台和离层形成部位组织中IAA、ABA含量;在采收前20d内定期测定了离层部位组织中细胞壁分解酶(Cellulase)的活性;在收获期测定了果实乙烯的发生量。结果表明:不同品种果柄、果台和离层部位组织中IAA和ABA含量变化有差异,但它们变化的总趋势相似,都是随着果实成熟IAA含量下降,而ABA含量上升;不同品种的成熟果实中乙烯发生量有很大差异,以落果多的品种显著大于落果少的品种;采前落果重的品种离层部位组织中细胞壁分解酶的活性在果实成熟期急剧增加。由于果实进入成熟阶段后,IAA含量下降,ABA含量升高,ABA/IAA之间的相对平衡被打破,高ABA/IAA以及高乙烯会刺激离层组织中细胞壁分解酶的活性增高,进而促进离层形成,这可能是导致落果发生的原因。     

不同含水量菜豆种子老化过程中生理特性的研究

 对不同含水量的菜豆种子( 2.1%、3.4%、3.8%、6.9%和9.0% ) 老化过程中的生理特性进行了研究。结果表明, 菜豆种子在含水量为318%时, 发芽率, 保护酶系统( SOD、POD和CAT) 活性和脱氢酶活性明显高于其它含水量的种子, 而脂氧合酶(LOX) 活性和丙二醛(MDA) 含量显著低于其它含水量的种子, 表现出较高的生活力。此结果表明适度降低菜豆种子的含水量可以提高其抗老化劣变能力;不同生活力的菜豆种子POD同工酶酶谱表现不同, 而SDS2PAGE蛋白质谱带则基本相同。     

难忘的起步阶段

时光似箭,日月如梭。转眼,中国饲料工业协会成立20周年了,中国饲料工业也走过了从起步到腾飞的光辉历程。1985年我开始接触这个新兴行业直到1995年退休。作为一名具体工作的行业管理工作者,我度过了从艰难创业到蓬勃发展的不平凡的十年。这其中有丰收的喜悦,也有创业的艰辛;有成功的快乐,也有阻力和冷漠带来的苦涩。回忆起步阶段的十年,酸甜苦辣,百感交集。从创业到蓬勃发展1985年,山东省政府决定全省饲料工业的统筹管理由省计委移交省经委后,我开始踏入这个新的战斗行列。省政府对饲料工业是重视的。早在1985年前就成立了全省饲料工业领导…