不同贮藏温度对大五星枇杷果实保鲜效果的研究

枇杷成熟于初夏高温多湿季节，果实生理活动旺盛，含水量多，极易腐烂变质，一般条件下难以贮存和长途运销，采后损失率高。目前，枇杷贮藏最常用的方法是冷藏法。郑永华等把枇杷果实在1℃低温贮藏，可显著抑制果实失水，贮藏期可达20天以上；Mukerjece认为，枇杷贮藏在3℃低温下能减少腐烂；周德荣通过0、5、15℃和常温比较表明，“照种”枇杷在5℃下贮藏31天的好果率最高为83．9％。但近年来，一些研究报道，枇杷果实在不适宜的低温下长期贮藏会发生冷害现象。曾雅鹃等发现，在1℃低温下贮藏，枇杷果皮难剥，果肉变硬，     

不同毒饵及性诱剂不同使用方法对桔小实蝇的诱杀效果试验

桔小实蝇Dacus dorsalis（Hendel）又称东方果实蝇、食心虫，在我区除为害多种水果外，还为害苦瓜、丝瓜等瓜类蔬菜，是近年我区果蔬作物的主要害虫之一。2004年，部分未套袋的番石榴、洋桃、桃、杨梅等水果及苦瓜、丝瓜等瓜类作物，果实受害率平均60％以上，重的达80％～90％。严重影响水果及瓜类的产量和品质。     

荧光PCR快速诊断一起新城疫

2005年1月底．广东省某规模养殖场的鸡出现了张口喘气、呼吸困难，有呼噜声、咳嗽，排黄绿色粪便等症状．部分鸡还有神经症状。发病约450只．发病率为1．2％。三天后鸡只陆续出现死亡．两天内死亡50只左右。解剖病死鸡，可见：喉头、气管粘膜明显出血，小肠轻度炎症。回肠、直肠、泄殖腔粘膜出血。临床无法确诊。经实验室快速诊断．为新城疫感染。     

Detection,cloning and expression of bone morphogenetic protein-1 from human osteosarcoma cell lines

AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen.     

甜玉米玉米螟的发生危害与防治措施

研究了吉林省公主岭地区甜玉米上亚洲玉米螟Ostrinia furnacalis(Guene)的发生危害和防治措施。田间抗螟性人工接虫鉴定结果表明,甜玉米710A和普通玉米自交系丹340的食叶级别为9级,即高感玉米螟,而单交种邢抗5号和邢抗2号的食叶级别为5级,为中抗型。与普通感虫和中等抗虫性玉米品种相比,取食甜玉米的玉米螟幼虫存活率高3～7倍,发育速度更快,提前约4～8天(1～2个龄期),说明甜玉米比普通玉米感虫。甜玉米比普通玉米及早播比晚播田更吸引玉米螟产卵。田间小区试验表明,化学杀虫剂的防治效果比白僵菌和苏云金杆菌的好,以氯菊酯喷雾防治效果最好,虫孔减退率达90％;其次是杀螟灵颗粒剂、Bt喷雾和Bt颗粒剂;而白僵菌的防治效果较差,喷雾防治效果好于颗粒剂。大田防治效果与小区试验基本相同。同时还分析了氯菊酯防治甜玉米玉米螟的防治策略、防治时期、施用次数等。此外,对Bt制剂作为化学杀虫剂的取代品及与其它生物防治方法协调使用的优点与潜力进行了讨论。     

不同碳源对虎奶菇菌核蛋白质营养价值的影响

测定了四种碳源对虎奶菇菌核蛋白质营养价值的影响。结果表明，以玉米淀粉为碳源，虎奶薅菌蛋白质的化学评分，必需氨基酸指数，生物价和营养指数分别为11．63、69．80、64．40和9．98，均居四种参试碳源的第一位；氨基酸评分和氨基酸比值系数分分别为14．55和12．45，均居四种参试碳源的第二位，这些结果证实，以玉米淀粉为碳源，虎奶菇菌核蛋白质营养价值高，值得推广应用。     

黄瓜无毛突变体的生理特性研究

 黄瓜无毛突变体与普通黄瓜相比, 幼苗的各种性状均没有明显变化; 成株矮化明显, 雌花数降低, 产量明显下降, 但雌花节率没有明显变化, 蒸腾速率、气孔导度较高, 其他光合作用指标没有明显变化。遗传研究表明, 无毛性状是由一对核基因控制的隐性性状。     

Effect of lansoprazole on gastric ulceration in rats

AIM:To investigate the effect of lansoprazole on gastric ulceration in rats. METHODS:Using the gastric ulcer model induced by hemorrhagic shock, restraint water-immersion stress and pylorus-ligature, the protective effect of lansoprazole (iv) on gastric ulceration was observed. RESULTS:Pretreatment with lansoprazole (7.5-60 mg/kg) significantly inhibited the formation of gastric ulcer in the three models in a dose-dependent manner. The autiulcer efficacy of lansoprazole was similar to that of omeprazole in the equal dose, but stronger than that of omeprazole for ulcer induced by water-immersion stress.CONCLUSION:The intravenously administered lansoprazole inhibited formation of experimental gastric ulcer in rats.     

30种中药抑菌活性的筛选试验初报

近十年来,从天然资源(即生物界包括微生物到昆虫、动植物)中寻找活性物质代替化学农药已成为植保研究的热点,因为已经发现了不少值得注意的抗菌剂是以它们的天然防御体系起作用的。从植物中寻找抑菌活性物质是开发无公害新农药的一条有效途径,     

笋用竹害虫天敌资源研究

本文报道了浙江省笋用竹害虫天敌资源调查的结果，共查明天敌资源44种，经鉴定属于螳螂目1科2种，半翅目1科2种，蛇蛉目1科1种，脉翅目1科2种，鞘翅目2科9种，双翅目2科2种，膜翅目9科22种；蛛形纲蜱螨目1科1种，蜘蛛目3科3种，指出了天敌的寄主和分布。