增蛋散对蛋鸡内分泌及消化作用的影响

将768只421日龄海兰白蛋鸡随机均分为2组,分别饲喂添加0和1.5%中药增蛋散的2种饲料,于饲养30、60d时采血和屠宰,测定血样中卵泡刺激素(FSH)、促黄体素(LH)、三碘甲状腺原氨酸(T3)、甲状腺素(T4)、雌二醇(E2)、孕酮(P4)、甲状旁腺素(PTH)、环磷酸腺苷(cAMP)等的含量及饲料中粗蛋白和钙的消化率,探讨该散剂促产蛋的作用机制.结果显示,当日粮中添加增蛋散饲喂30、60 d时,蛋鸡血清中FSH、LH、T3、T4、E2、P4、PTH的含量,下丘脑和腺垂体中cAMP含量及饲料中粗蛋白和钙的消化率均有显著提高(P＜0.05或P＜0.01).本试验结果为该散剂提高产蛋后期鸡的产蛋率,推迟其淘汰期的药理作用提供了实验依据.     

Cutaneous lymphoma in a juvenile dog

Abstract: An 18-month-old male Doberman Pinscher was referred to the Veterinary Medical Teaching Hospital of the College of Veterinary Medicine for an erythemic nodular mass on the right forelimb. The mass was diagnosed as cutaneous lymphoma, based on cytologic examination of a mass aspirate and histopathology. Using immunohistochemistry the neoplastic cells were positive for CD3 but negative for CD79a, E-cadherin, and pancytokeratin, confirming their origin as T lymphocytes. No tumor recurrence was noted 18 months after surgery. To our knowledge, this is the first report of a solitary nodular form of cutaneous lymphoma in a young dog.     

海南坡鹿栖息地的外来植物调查

海南坡鹿是中国特有的国家Ⅰ级重点保护野生动物,其栖息地的稳定和发展对该物种种群有重要意义。潜在的外来入侵植物会对栖息地产生长期不可恢复的影响。因此,我们于2009—2011年采用样方调查和线路调查相结合的方法对海南坡鹿的栖息地海南大田国家级自然保护区内的外来植物进行了调查,共记录外来植物40科95属115种。其中,草本植物80种,占总物种数的69．6％;不论从科区系还是属区系上看,外来植物的区系分布都具有热带性;50．4％的外来植物产自美洲,在草地中分布最多。保护区内分布广泛的外来植物已对乡土物种产生一定的影响,也将可能对坡鹿的食物资源产生不利影响。根据调查结果,我们提出了一些保护管理建议。     

番鸭源鸭疫里默氏菌大环内脂类药物的耐药基因检测与分析

为了解莆田市番鸭源鸭疫里默氏菌对大环内酯类药物的耐药性和相关耐药基因携带情况,对本实验室分离鉴定的49株番鸭源鸭疫里默氏菌菌株采用K-B纸片扩散法进行药敏试验,提取菌株基因组DNA,采用PCR方法对大环内酯类药物的ermA、ermB、ermC、ermF、ereD等5种耐药基因进行检测,阳性产物送公司测序并对序列进行分析...     

江苏常州地区禽白血病血清学调查研究

为了解江苏常州地区肉鸡禽白血病病毒(ALV)感染情况,本研究采集了该地区2019年7-12月、2020年1-4月肉鸡血清样本1257份,通过ELISA试剂盒检测ALV-A/B、ALV-J、抗原p27阳性率。结果显示：在接受调查的两个地区均存在ALV感染及混合感染,且ALV-A/B感染率(13.13%)均高于ALV-J(5.01%);不同月份阳性率存在一定差异,ALV-A/B、ALV-J均为春夏季阳性率较高,秋冬季逐渐下降趋势;抗体阳性样本中有54.39%的p27抗原阳性率。本次调查为江苏常州地区商品肉鸡ALV净化及防控提供一定的参考依据。     

刚棘颚口线虫的扫描电镜观察

刚棘颚口线虫扫描电镜显示：头球顶端有两片半球形的大侧唇，大侧唇背面有1对双乳突和1个头感器。在轮节皮棘第6-7排或第8-9排或第10-11之间各有1对颈乳突，与樊氏等及古贺正崇等描述颈乳突位置有所差异。并观察到雄虫尾端长交合刺的皮纹上有细小的凹陷小乳，其顶端有开口处；有2对尾乳突。雌虫阴门位于虫体腹面中线之后；尾端前腹面有半月状的肛门，有1对尾乳突。其中头钩、体棘、交合刺和尾乳突等形态特征与前人记述相似。     

猪圆环病毒2型感染PK-15细胞差异表达miRNA和mRNA互作网络

旨在筛选猪圆环病毒2型感染猪肾上皮细胞(PK-15)后差异表达miRNA和mRNA,并探究miRNA和mRNA之间的互作关系。使用茎环定量PCR检测多个热点miRNA在PCV2感染组和未接种病毒组之间的差异表达水平以发现差异表达miRNA,分析PCV2感染后第12小时的蛋白质组学研究结果(GEO登录号:GSE71945)以获得差异表达mRNA,并分析差异表达miRNA和mRNA之间的互相作用关系。结果显示,在PCV2感染后第12小时miR-10b、miR-128、miR-155-5p、miR-21、miR-29b、miR-361-3p等表达量显著性变化,使用miRecords预测其靶基因,分别发现7 105、9 741、7 451、7 183、7 971、8 793个靶基因;PCV2感染后第12小时共有158个差异表达mRNA,分属于多种细胞结构成分并参与多条重要的细胞生物途径,且差异miRNA的靶基因和差异mRNA之间有大量的交集基因。上述结果表明,PCV2感染能导致细胞内多种miRNA差异表达,造成miRNA的靶基因表达紊乱,进而影响细胞正常的生命进程。     

主要蛋鸡养殖国家蛋鸡粪处理概况及其对我国的启示

畜禽粪污是畜牧业环境污染的重要组成部分,已对我国生态环境可持续发展产生严重影响,同时也严重制约了产业的进一步发展.本文重点对美国、日本和欧盟为代表的发达国家的蛋鸡粪处理进行系统分析,并在此基础上探索上述国家和地区的模式时我国蛋鸡粪处理的启示.     

Study on some molecular characterization of Babesia orientalis

The study on buffalo babesiosis indicated that its pathogen was different from other Babesia on many aspects such as morphology, transmission and pathogenicity. Therefore, it was named as a new species—Babesia orientalis. In order to prove the validity of this taxon, molecular taxonomic study on the pathogen was done in this experiment. The complete 18S rRNA gene sequence of B. orientalis was determined by PCR. It was sequenced and blasted. The results indicated that the classification of the parasite belonged to the genus Babesia. The 1700 bp complete sequence was compared with 15 other Babesia sp. available in GenBank. The data were analyzed and a phylogenetic tree was established. The results indicated that the hereditary distance of the parasite was close to that of Babesia sp. from South Africa and Babesia ovis, and the hereditary distance was far from Babesia bigemina and B. bovis.     

Molecular Cloning and Expression of IL2 cDNA from the Tibet Pig

Interleukin-2 is a vital cytokine secreted by activated T lymphocytes, and plays important role in the regulation of cellular and humoral immunity of animals. In our experiment, IL2 cDNA of the Tibet Pig was first cloned by RT-PCR from ConA-stimulated lymphocytes in the blood and subcloned into pMD-18 T vector, which then was identified with endonuclease restriction. The sequencing result showed that Tibet pig IL-2 (TPIL-2) cDNA was 503 bp long (ORF was 465 bp) (Genbank accession number: AY 294018). The recombinant prokaryotic and eukaryotic expression plasmids of the cDNA were then constructed to analyse the ability to stimulate the proliferation of porcine lymphocytes in vitro. The recombinant porcine IL-2 expressed in the prokaryotic cells was found to be of 43 kDa molecular mass, which was consistent with a 17.4 kDa protein deduced from the IL-2 cDNA sequence (glutathione S-transferase molecular mass is 26 kDa); the recombinant protein in eukaryotic cells was confirmed by use of specific rabbit anti-porcine IL-2 serum in an ELISA. The bioactivity of TPIL-2 was detected through MTT colorimetry by stimulating the proliferation of pig ConA-stimulated blasts in vitro. The results indicate that the TPIL-2 significantly promoted the proliferation of ConA-stimulated blasts of pig. This confirms that IL-2 cDNA of the Tibet pig was successfully cloned and expressed in prokaryotic and eukaryotic cells, which lays the foundation for the the preparation of specific recombinant IL-2 protein and development of novel immune adjuvants to raise the immunity of pigs against various infectious pathogens and increase the immunoprotective efficacy of vaccines.