Efficacy of different dosages of ivermectin injectable against the Hypoderma spp. in yaks

A chemotherapy trial was conducted to determine the lowest dosage of injectable preparation of ivermectin against Hypoderma spp. infestation in yaks in Tibetan areas in Tianzhu county, Gansu province, in northwest of China. One hundred and sixty yaks were randomly divided into four groups of 40 yaks for the trial. The first three groups were treated by subcutaneous injection in the neck with 0.1% ivermectin (respectively, 1, 5, 10 microg/kg body weight). The fourth group was not treated and considered as control group. All the experiments were performed in November 2000 and the animals were examined for the presence of warbles in the next March and May. The results indicated that there was no warbles found on the back of treated animal while third stage larvae were palpated on back of some of the yaks in control group. It is concluded that dosage of 1 microg/kg ivermectin injectable was sufficient to kill or stop development of larvae of Hypoderma spp. in naturally infected yaks if administrated in November.     

Efficacy of injectable and pour-on microdose ivermectin in the treatment of goat warble fly infestation by Przhevalskiana silenus (Diptera, Oestridae)

The prophylactic efficacy of microdoses of injectable and pour-on ivermectin formulations against larval stages of Przhevalskiana silenus was assessed in naturally infected goats in the region of Calabria (southern Italy).Sixty-eight goats from two goat farms were divided into five groups: one group remained untreated, while the other four groups were treated with microdoses of ivermectin (5 and 10 microg/kg injectable formulation and 10 and 20 microg/kg pour-on formulation).The microdoses of ivermectin were fully effective in the treatment of goat warble fly infestation (GWFI) as no larvae emerged from the warbles in the treated groups, while all the larvae emerged in the control groups. Irrespective of the type of formulation used, the difference between the treated groups and the control group was statistically significant (P< 0.001). By contrast, no statistical differences were found between the goats treated with the injectable formulation and those receiving the pour-on applications, and between the two doses of the injectable and pour-on formulations used. Given the plasma concentrations it attains at its lowest dose (0.052 - 0.042 ng/ml for the injectable formulation and 0.030 ng/ml for the pour-on) the injectable formulation seems to offer the most reliable route for the administration of ivermectin microdoses and it is acceptable for milk consumption. The introduction of ivermectin in the early eighties and the use of microdoses in some cases have made it possible to control cattle hypodermosis in large areas of Europe. As with cattle hypodermosis, the administration of ivermectin microdoses in goats is particularly interesting because of the low costs involved and the low levels of residues found in goat milk; it may thus constitute the basis for GWFI control campaigns in areas where the disease is prevalent.     

Early hepatic immune response in rats infected with Fasciola hepatica

We investigated the phenotype of the T cells (CD4+ and CD8+) that produced Th1 (IFN-gamma) and Th2 cytokines (IL-4 and IL-10) during the firsttwo weeks of experimental fasciolosis in rats. We also followed the kinetics of the cytokine and proliferative responses of hepatic mononuclear cells (HMNC) over the same period. We found that HMNC were more numerous in the infected animals than in the controls. The percentage of CD4+ cells increased significantly after infection, whereas the percentage of CD8+ cells did not change. Moreover, the frequency of the cells producing (CP) cytokine changed after infection. The frequency of CP IFN-gamma on 7 days postinfection (pi) was similar to that in control animals. However, the frequency of CP IFN-gamma was clearly lower on day 14 pi, whereas the frequency of CP IL-4 and CP IL-10 had increased. The CP IL-10-were mostly CD4+. Mitogenic stimulation (phorbol myristate acetate/ionomycin) of HMNC led to an increase in the amounts of the Th2 cytokines in the supernatant on days 7 and 14 pi, with the increase more pronounced on day 14. In contrast, IFN-gamma levels also increased by day 7 pi but then decreased to below control levels by day 14. In addition, HMNC proliferation in response to mitogen followed a similar pattern to IFN-gamma production. These findings suggested that, during the first 2 weeks of infection, F hepatica induced a transient ThO cytokine profile followed by downregulation of the cellular response and the induction of a Th2 cytokine profile.     

Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats

The in situ distribution of NK cells in rat liver during the first 28 days of an experimental infection with F hepatica was investigated. NK cells were distributed homogeneously throughout the hepatic parenchyma in uninfected animals. The total number of hepatic mononuclear cells increased significantly following infection, but the proportion of NK cells did not change. After infection, these cells were found around the portal space, around the centrolobular vein, in the periportal fibrosis and in the band of collagen. However, no NK cells could be detected in or around the granuloma during infection. The frequency of both I L-2- and IFNgamma-producing NK cells was higher on day 7 postinfection (pi) but only the percentage of IFNgamma -CD161+ subsets remained elevated thereafter, whereas the percentage of both IL-2+CD161+ and IL-4+CD161+ subsets returned to the baseline. The number of CD161+IL10+ cells did not change significantly. These results suggest that NK cells could be another source for the early production of IFNgamma but provide no evidence that these cells are involved in early events associated with granuloma formation.     

Effect of Ultraviolet Disinfection of Hydroponic Solutions on Pythium Root Rot and Non-target Bacteria

Dispersal of root pathogens is a major concern in closed hydroponic cultures. To limit dispersal, ultraviolet (UV) disinfection technology has been used to remove pathogens but its effect on non-target bacterial populations is largely unknown. In this study, the circulating solution was disinfected with various UV doses (i.e., 19, 38, 59, 88mJcm^–2) before recirculation. At the beginning of the experiment, the hydroponic solution was inoculated with Pythium aphanidermatum at 6.7±1.5CFUmL^–1. Four months later the population density of P. aphanidermatum reached 1030CFUmL^–1 in the control and 1028, 970, 610, and 521CFUmL^–1 in the solutions treated by the above UV doses. In all UV-treated solutions, significant (P<0.05) reduction of both Pythium and bacterial populations was noted. For the former, its reduction did not result in decrease of Pythium root rot nor increase of tomato fruit yield. For the latter, its reduction showed a concomitant decrease of the populations in the rhizosphere. The bacterial populations in the rhizosphere were 6.77×10⁹CFUmg^–1 fresh roots at the beginning of the experiment and were 7.89×10⁸, 9.93×10⁷, 7.33×10⁷, and 3.51×10⁷CFUmg^–1 fresh roots at the end of the experiment in the control, UV38, UV59, and UV88 treatments, respectively. The bacterial density also decreased with time in the control (UV0) although at a low rate. The results suggest that the attempt to control Pythium root rot by UV irradiation of recirculating solutions to remove P. aphanidermatum also affects the non-target bacterial populations in the rhizosphere. The interaction between the target pathogen and non-target bacterial flora in UV-treated hydroponics needs further investigation.     

我国农药科学之发展

中国是世界农药生产大国,生产能力已达100万吨左右,约1/2产品出口到世界农药市场。半个多世纪以来中国农药的发展趋势基本上与世界农药的发展同步。近年来已开始自主合成新农药,有21种已获得中国专利。农药毒理学是农药研究开发的重要基础研究领域,是新农药研究开发之本,不论在新化合物的合成筛选还是农药的剂型和制剂开发利用方面,生理毒理学和宏观毒理学的深入研究都将为新农药的研究开发提供强大的推动力。本文介绍了世界某些重要农药生产公司在新农药研发方面的成功经验,可供中国新农药和农药剂型及制剂研究开发部门共享。     

Lack of biocontrol capacity in a non-pathogenic mutant of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">melonis</Emphasis>

The aim of this study was to assess the biocontrol capacity of rev157, a non-pathogenic mutant of a pathogenic strain of Fusarium oxysporum f. sp. melonis (Fom24). Inoculated in association with the virulent parental strain, the mutant rev157 did not protect the host plant (muskmelon) against infection by Fom24. Applied on flax, a non-host plant, the mutant rev157 was not able to protect it against its specific pathogen F. oxysporum f. sp. lini. On the contrary the parental strain Fom24 did protect flax as well as a soil-borne biocontrol strain (Fo47). Since the mutant rev157 was affected neither in its growth in vitro nor in its capacity to penetrate into the roots, it can be speculated that the mutation has affected traits responsible for interactions within the plant. In F. oxysporum the pair of strains Fom24/rev157 is a good candidate to identify genes involved in the biocontrol capacity of F. oxysporum and to test the hypothesis of a link between capacity to induce the disease and capacity to induce resistance in the plant.     

四倍体刺槐的饲用营养价值分析

该研究测定了四倍体刺槐常规营养成分、微量元素、维生素、氨基酸、单宁等的含量,并与苜蓿、羊草等作了对比.四倍体刺槐叶粉干物质中的粗蛋白含量达到24.6%,粗纤维含量为13.6%,可作为蛋白质饲料使用.综合各项指标,认为四倍体刺槐是一种较好的粗饲料,可在反刍动物饲料中替代苜蓿.     

板栗良种基地营建技术研究

在江苏省溧阳市龙潭林场，进行了板优良品种的收集，良种优良，良种繁育，板栗嫩枝扦插等一系列营建技术研究，取得了明显的经济，社会效益。     

秦岭松栎林带生物量及其营养元素分布特征

秦岭脱齿栎林、油松林和华山林（包括０－６０cm土层）营养元素总贮量达２９．２５００－３９０．１７３９t.hm^-2，其大小为锐齿栎林＞华山松＞油松林，土壤中营养现贮量占系统总量的９３．１０％－９９．３９％，植被层和凋落物层仅占０．５７％－３．００％和０．１０％－１．９９％；３林分乔木层生物量、木材蓄积量和营养元素积累量平均值分别为１２０．４８３t.hm^-2,129.384m^3.hm^-2和１２２６．９kg.hm^-2，锐齿栎林＞油松林＞华山松林。３林分林下植被层生物量现存量和营养元素积累量平均值分别为１．５５３t.hm^-2和５３．１kg.hm^-2，锐齿栎林最大，华山松林最小。林下凋落物现存量和营养元素积量平均值分别为１７．４７５t.hm^-2和５０２．５kg.hm^-2，３林分凋落物现存量、营养元素含量及其积累量都存在明显的差异。锐齿栎林、油松林和华山松林营养元素年吸收量、归还量、存留量和平均归还率分别为３３．４、１４７．２和２６４．３，１９５．６、６６．９和８４．１，１３８．８、８０．４和１８０．２kg.hm^-2和０．５８５、０４５４和０．３１８，３林分各营养元素的年吸收量，归还量和存留量也存在一定的差异，锐齿栎林干和皮中Ｃa和Ｍg含量远远高于油松林和华山松林的，锐齿栎林多代连作势必导致营养元素的大量流失，特别是Ｃa和Ｍg，会破坏土壤原有离子动态平衡，引起林地土壤理化性质改变，林地生产潜力逐渐下降。