牛AR基因5′UTR SNP分析及与精液品质关系

采用PCR-SSCP方法对种公牛群体雄激素受体(AR)基因5′非翻译区(5′UTR)多态性进行检测。结果表明:AR基因5′UTR含有多态性位点;测序结果显示5′UTR的-1575处存在T→G碱基突变;用最小二乘方法对该基因型与生产性状相关分析显示遗传多态性与精子密度性状呈显著相关,AA型显著高于AB型(P=0.025),对其他性状的影响差异不显著。试验为研究种公牛体内AR基因的生物学作用奠定了基础,为肉用种公牛的早期选种选育提供了候选基因和分子标记。     

图书馆危机管理流程分析

危机管理作为图书馆管理内容的重要组成部分,影响着图书馆的生存与发展,影响着图书馆的社会声誉与社会地位。文章分析了图书馆危机管理的概念,从流程管理视角出发,依次分析图书馆危机管理中的日常危机管理、危机事件管理、危机后续管理3个流程。     

农业网络资源的开发与利用

本文阐述了开发利用网上农业信息资源的重要意义，并对开发利用的方法与途径进行了探讨。     

额济纳旗绿洲生态环境的遥感动态监测分析

在干旱区利用光学遥感可以对其沙化状况、治沙效果进行监测 ,得到良好的监测效果。利用 L and-sat TM遥感图像并结合 GIS分析了绿洲最近 15 a来额济纳绿洲生态环境变化情况 ,分析表明额济纳沙漠化土地已遍布全旗。( 1)绿洲北部、东部等的湖泊水体范围在锐减 ;( 2 )绿洲面积在明显减少。2 0 0 0年比1986年后 15 a来绿洲面积减少了 6 1.48% ,平均每年面积递减 4.39% ;( 3)绿洲质量及盖度在下降。绿洲生态环境质量下降的主要原因是人类对水资源与土地的不合理利用 ,因此 ,通过引进现代高新技术如遥感的监测与系统地分析及科学地治理 ,额济纳旗绿洲生态恢复是完全可能的     

Occurrence of Honeysuckle Yellow Vein Virus (HYVV) containing a monopartite DNA-A genome in Korea

Two newly emerged begomoviruses were isolated from naturally infected tomato (Solanum lycopersicum) plants grown in greenhouses at Jeju Island and Dangjin in Korea and their genomes were characterized. These viruses-infected plants had very small leaves that curled upward, yellow margins and a leathery appearance, and a bushy and stunted appearance with short internodes. Nucleotide (nt) sequence analysis of their genomes showed that they have a DNA-A component of a monopartite begomovirus. Their genomes comprised 2763 and 2764 nucleotides with six open reading frames. The results of nt sequence similarity analysis of DNA-A genome between the two Korean isolates and isolates of Tobacco leaf curl Japan virus (TbLCJV), Honeysuckle yellow vein virus (HYVV), Honeysuckle yellow vein mosaic virus (HYVMV), and Eupatorium yellow vein virus in Japan (EpYVV) showed that they are likely similar to HYVV-[Masuda] (89.4–92.8% nt identity). Consequently, we tentatively propose the two isolates’ names as HYVV-Jeju and -DJ according to the ICTV geminivirus rules. Phylogenetic relationship analysis of 33 DNA-A genome sequences using PAUP* 4.0b10 and MrBayes revealed that HYVV-Jeju and -DJ belong to the Far East Asian begomovirus species complex. Within the Far East Asian begomovirus species complex, HYVV-Jeju and -DJ are distantly related to EpYVV, HYVMV, and TbLCJV groups. Based on the presence of a recombination fragment spanning the C3 ORF, a recombinant origin was suggested for both HYVV-Jeju and –DJ, with parents close to Japanese isolates HYVMV-[SP1:00] and Eupatorium yellow vein virus (EpYVV)-[Suya]. In addition, the presence of a further recombination fragment spanning the IR suggested the parents of HYVV-DJ were close to HYVV-Jeju and EpYVV-[Suya].     

CFD-DEM耦合模拟网式过滤器局部堵塞

过滤器内部的流场不均导致使用时容易产生局部堵塞,堵塞分布受入口流速、颗粒粒径、流线轨迹等因素的共同影响。该文以计算流体力学-离散元法(computational fluid dynamics-discrete element method,CFD-DEM)耦合模拟不同流量下Y型网式过滤器内部不同粒径的沙粒运动及分布,分析过滤器内部流态对沙粒运动分布的影响并通过试验加以证明。结果表明,滤网两侧的压差占总压差的77%。网面流量呈阶梯分布,最大流量位于出口侧滤网上端,最低流量位于进口侧滤网中心,前者是后者的5.9倍;对于通过滤网的颗粒,入口流速越高,颗粒通过点越集中;对于拦截颗粒,当粒径接近孔径时,颗粒稳定附着在滤网,增加入口流速使颗粒向侧面滤网聚积并产生局部堵塞,粒径远大于孔径时,颗粒在内腔中不停运动,难以稳定附着在滤网;降低入口流速将提高颗粒分布的均匀程度,延长过滤器高效段时间,减少冲洗难度。     

利用转录组测序技术分析ADC和NOS基因mRNA在鹅等级前卵泡中的表达

为研究精氨酸代谢的关键酶精氨酸脱羧酶(Arginine Decarboxylase,ADC)和一氧化氮合酶(Nitric-oxide Synthase,NOS)mRNA在鹅等级前卵泡中的表达情况。采用高通量测序技术对鹅等级前卵泡进行转录组测序分析,并通过荧光定量PCR对结果进行验证。通过FPKM值计算分析,这2个基因在鹅等级前卵泡中均有表达,且随着卵泡的发育,NOS mRNA的表达呈现先降低后增加再降低的趋势,在初级卵泡中的表达量达到最高值,在中白卵泡中的表达量最低。ADC mRNA的表达则呈现先增加后降低再增加的趋势,在初级卵泡中的表达量最低,在小白卵泡中的表达量最高。荧光定量PCR结果与转录组测序结果基本相同。     

Expression in Escherichia coli of open reading frame gene segments of HTLV-III

Human T-cell lymphotropic virus type III (HTLV-III), the causative agent of the acquired immune deficiency syndrome (AIDS), was recently isolated and its genomic structure analyzed by DNA cloning methods. In the studies reported here a combined cloning and expression system was used to identify HTLV-III encoded peptides that react immunologically with antibodies in sera from AIDS patients. Cloned HTLV-III DNA was sheared into approximately 500-base-pair fragments and inserted into an "open reading frame" expression vector, pMR100. The inserted DNA was expressed in Escherichia coli transformants as a polypeptide fused to the lambda CI protein at its amino terminus and to beta-galactosidase at its carboxyl terminus. Sera from AIDS patients containing antibodies to HTLV-III were then used to screen for immunoreactive fusion proteins. Twenty clones, each specifying a fusion protein strongly reactive with AIDS serum, were identified. DNA sequence analysis indicated that the HTLV-III fragments were derived from the open reading frame DNA segments corresponding to the gag and pol gene coding regions and also the large open reading frame region (env-lor) located near the 3' end of the viral genome.     

基于XPS的苹果表皮残留农药界面层的氩刻分析

针对果皮表面残留农药进行微观深度探测及分析。结果表明,苹果表皮氧乐果及敌敌畏随施药浓度及施药时间的残留沿纵向深度变化呈现非线性规律性,主要残留部位为果实表皮的角质层区距表面8μm,氧乐果残留分布的规律性强于敌敌畏。