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121.
Munn DH Sharma MD Lee JR Jhaver KG Johnson TS Keskin DB Marshall B Chandler P Antonia SJ Burgess R Slingluff CL Mellor AL 《Science (New York, N.Y.)》2002,297(5588):1867-1870
Antigen-presenting cells (APCs) can induce tolerance or immunity. We describe a subset of human APCs that express indoleamine 2,3-dioxygenase (IDO) and inhibit T cell proliferation in vitro. IDO-positive APCs constituted a discrete subset identified by coexpression of the cell-surface markers CD123 and CCR6. In the dendritic cell (DC) lineage, IDO-mediated suppressor activity was present in fully mature as well as immature CD123+ DCs. IDO+ DCs could also be readily detected in vivo, which suggests that these cells may represent a regulatory subset of APCs in humans. 相似文献
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Matthew D. Barnhart DVM MS Diplomate ACVS Cristobal F. Rides DVM Shawn C. Kennedy DVM Diplomate ACVS Sean W. Aiken DVM MS Diplomate ACVS Charles M. Walls DVM Diplomate ACVS Christopher L. Horstman DVM MS Diplomate ACVS Jonathon C. Chandler DVM MS Diplomate ACVS Jeff D. Brourman DVM MS Diplomate ACVS Sean M. Murphy DVM Diplomate ACVS Frederick Pike DVM Diplomate ACVS Steven J. Naber MS PhD 《Veterinary surgery : VS》2013,42(1):60-66
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R.M. Barber Q. Li P.P.V.P. Diniz B.F. Porter E.B. Breitschwerdt M.K. Claiborne A.J. Birkenheuer J.M. Levine G.J. Levine K. Chandler P. Kenny P. Nghiem S. Wei C.E. Greene M. Kent S.R. Platt K. Greer S.J. Schatzberg 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2010,24(2):372-378
Background: Vector‐transmitted microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, Bartonella, and Borrelia are commonly suspected in dogs with meningoencephalomyelitis (MEM), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (CSF) of dogs with MEM is unknown. Hypothesis/Objectives: To determine if DNA from these genera is present in brain tissue and CSF of dogs with MEM, including those with meningoencephalitis of unknown etiology (MUE) and histopathologically confirmed cases of granulomatous (GME) and necrotizing meningoencephalomyelitis (NME). Animals: Hundred and nine dogs examined for neurological signs at 3 university referral hospitals. Methods: Brain tissue and CSF were collected prospectively from dogs with neurological disease and evaluated by broadly reactive polymerase chain reaction (PCR) for Ehrlichia, Anaplasma, Spotted Fever Group Rickettsia, Bartonella, and Borrelia species. Medical records were evaluated retrospectively to identify MEM and control cases. Results: Seventy‐five cases of MUE, GME, or NME, including brain tissue from 31 and CSF from 44 cases, were evaluated. Brain tissue from 4 cases and inflammatory CSF from 30 cases with infectious, neoplastic, compressive, vascular, or malformative disease were evaluated as controls. Pathogen nucleic acids were detected in 1 of 109 cases evaluated. Specifically, Bartonella vinsonii subsp. berkhoffii DNA was amplified from 1/6 dogs with histopathologically confirmed GME. Conclusion and Clinical Importance: The results of this investigation suggest that microorganisms in the genera Ehrlichia, Anaplasma, Rickettsia, and Borrelia are unlikely to be directly associated with canine MEM in the geographic regions evaluated. The role of Bartonella in the pathogenesis of GME warrants further investigation. 相似文献
125.
Ireland JL Clegg PD McGowan CM McKane SA Chandler KJ Pinchbeck GL 《Equine veterinary journal》2012,44(1):94-100
Reasons for performing study: Previous studies suggest that owners underestimate or incorrectly recognise or report health problems in geriatric horses. However, few studies have directly compared owner‐reported and veterinary assessed disease. Objectives: To compare the findings of veterinary clinical examination of geriatric horses with owner‐reported clinical signs and disease. Methods: A total of 200 horses aged ≥15 years were randomly selected to receive a veterinary examination, from responses to a cross‐sectional postal questionnaire survey. Veterinary examinations were performed within 2 months of questionnaire return, and agreement between owner‐reported data and veterinary clinical findings was assessed. Results: Owners under‐reported many clinical signs and disease conditions detected on veterinary clinical examination. For example, dental abnormalities (detected in 95.4% of horses, reported by 24.5% of owners); cardiac murmurs (detected in 20% of horses, reported by 0.5% of owners); lameness (present in 50% of horses, reported by 23% of owners) and hoof abnormalities (detected in 80% of horses, reported by 27% of owners). Agreement between owner‐reported and veterinary assessed respiratory disease (Kappa 0.02–0.2), body condition score (Kappa 0.24) and coat abnormalities (Kappa 0.42) was poor, fair and moderate, respectively. Range of motion (ROM) of the tarsal and metacarpophalangeal joints was lower in horses with owner‐reported osteoarthritis (P = 0.005 and <0.001, respectively). Conclusions and potential relevance: The low prevalence and relatively poor agreement of owner‐reported disease compared to that detected on veterinary examination suggests inaccurate or under‐recognition, or inaccurate reporting of health problems by owners of geriatric horses, which could lead to a delay in presentation for veterinary treatment. Increased veterinary involvement and improved owner education in the care of geriatric horses should facilitate earlier identification of disease, particularly that which is not readily detectable by owners, and aid management of health and welfare problems. 相似文献
126.
Wiley PR Tanner GJ Chandler PM Anderssen RS 《Journal of agricultural and food chemistry》2009,57(10):4042-4050
Near-infrared reflectance (NIR) spectroscopy was used in the characterization of grain morphology mutants of barley ( Hordeum vulgare L.) in relation to grain nitrogen (N) content and protein composition. Derivative spectroscopy provided spectra with enhanced resolution, allowing wavelengths to be identified with clear differences in contribution from associated chemical bonds. Comparisons of fourth-derivative spectra of wholemeal flour from high-N grains with flour from low-N grains identified wavelengths at which there were statistically significant differences between the groups. Their importance was independently confirmed by step-up regression using these wavelengths to generate an equation predicting N content (R(2) = 0.98). Fourth-derivative spectral comparisons also allowed novel biochemical differences to be predicted. Visual assessment of the spectra of all mutants revealed a variable region (1470-1520 nm, corresponding to N-H stretch vibrations) that allowed two extreme sets to be defined. The protein extracted from these two sets differed markedly in hordein content. 相似文献
127.
Subconjunctival antimicrobial poloxamer gel for treatment of corneal ulceration in stranded California sea lions (Zalophus californianus)
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Chandler LD 《Pest management science》2003,59(6-7):605-608
The corn rootworm areawide management program was implemented by USDA-ARS in 1995 at five locations across the USA. This program is based on the use of a semiochemical insecticide bait applied to maize (Zea mays L) during peak adult corn rootworm activity. Managing adult rootworms minimizes the number of eggs laid, resulting in fewer larvae available to economically damage maize roots in the following growing season. To date, rootworm populations have been significantly reduced at all participating locations and new bait products have been developed and evaluated for use in rootworm-infested areas. 相似文献