Prevention of organ allograft rejection by a specific Janus kinase 3 inhibitor

Because of its requirement for signaling by multiple cytokines, Janus kinase 3 (JAK3) is an excellent target for clinical immunosuppression. We report the development of a specific, orally active inhibitor of JAK3, CP-690,550, that significantly prolonged survival in a murine model of heart transplantation and in cynomolgus monkeys receiving kidney transplants. CP-690,550 treatment was not associated with hypertension, hyperlipidemia, or lymphoproliferative disease. On the basis of these preclinical results, we believe JAK3 blockade by CP-690,550 has potential for therapeutically desirable immunosuppression in human organ transplantation and in other clinical settings.     

贵妃鸡生长发育性状的测定与分析

为研究贵妃鸡体重及体尺间的相关性,对60只贵妃鸡的早期生长发育规律及13周龄体尺性状进行了测定,并对测定指标进行分析.结果表明:在0～13周龄,贵妃公鸡平均体重极显著高于母鸡.由生长曲线图可知,在7～8周龄时公鸡增重达到最大.13周龄时贵妃公母鸡平均体重分别为1.1130 kg和0.8954 kg,平均胫长分别为7.85 cm和6.81 cm,贵妃公鸡体重性状和体尺性状相关性极显著高于母鸡.     

抚仙湖北部农田区不同施肥对水稻产量、氮素吸收及利用率的影响

针对抚仙湖北部农田区的蔬菜施肥过量导致土壤养分残余量大,会对后作水稻施肥造成影响的问题,采用田间小区开展不同施肥试验研究。结果表明,砂壤土和粘壤土在施氮量为150~360 kg/hm²范围内,水稻植株氮素积累量随着施氮量的增加而提高,而增加的氮素积累量主要表现在茎叶部位;穗肥施用氮肥可提高籽粒氮素积累量。水稻氮肥吸收利用率和产量,砂壤土以施氮255 kg/hm2时最高,分别为45.1%和10594 kg/hm²;而粘壤土施氮150~360 kg/hm²之间,氮肥吸收利用率为20.9%~22.4%之间,产量为10486~10596 kg/hm2之间;当对砂壤土和粘壤土的水稻穗肥施用氮肥时,水稻氮肥吸收利用率（分别为42.8%、23.5%）和产量（分别为10445 kg/hm²、10564 kg/hm²）最高。水稻的氮素收获指数、氮肥农学利用率、氮肥生理利用率及氮肥偏生产力,均以砂壤土明显高于粘壤土,且随着施氮量的增加而显著下降。蔬菜后作水稻施氮量以150~255 kg/hm²范围为宜,氮肥分基肥50%+分蘖肥30%+穗肥20%施用     

适宜水稻花粉萌发的液体培养基的优化

花粉萌发率是检测花粉活力的一个重要指标,但实践中还缺少便捷、可靠的水稻花粉萌发率测定方法。本研究在前人研究基础上,采用液体培养基对相关培养条件进行了优化。结果表明,采用155g/L蔗糖+20mg/L H3BO3+40mg/L CaCl2+5 mg/L VB1相配合的培养基较适合两系杂交中稻两优818花粉的萌发,萌发率最高可达63.78%。此外,该培养方法具有培养条件要求不高、发芽结果稳定性好、培养基分布均匀等优点。     

Ecological effects of climate fluctuations

Climate influences a variety of ecological processes. These effects operate through local weather parameters such as temperature, wind, rain, snow, and ocean currents, as well as interactions among these. In the temperate zone, local variations in weather are often coupled over large geographic areas through the transient behavior of atmospheric planetary-scale waves. These variations drive temporally and spatially averaged exchanges of heat, momentum, and water vapor that ultimately determine growth, recruitment, and migration patterns. Recently, there have been several studies of the impact of large-scale climatic forcing on ecological systems. We review how two of the best-known climate phenomena-the North Atlantic Oscillation and the El Ni?o-Southern Oscillation-affect ecological patterns and processes in both marine and terrestrial systems.     

Generation of Salmonella ghost cells expressing fimbrial antigens of enterotoxigenic Escherichia coli and evaluation of their antigenicity in a murine model

Salmonella Typhimurium ghost cells expressing K88ab, K88ac, K99, and FasA fimbriae of enterotoxigenic Escherichia coli (ETEC) in their envelopes were constructed. The genes encoding the fimbriae were individually cloned into an expression plasmid, pMMP81, carrying the asd gene, which was subsequently electroporated into the Δasd S. Typhimurium mutant. Plasmid pJHLP99, carrying the phiX174 lysis gene E, was also subsequently electroporated into the Salmonella mutant. The presence of the individual fimbriae on the ghost cells was examined by Western blot analysis. Forty BALB/c mice were equally divided into 2 groups of 20 mice each. Group A mice were intramuscularly vaccinated with a mixture of the 4 ghost cells expressing the individual fimbriae. The group B mice were inoculated with sterile phosphate-buffered saline as a control. The antigen-specific serum IgG concentrations were significantly higher in group A than in group B from week 2 until week 6 after inoculation. In addition, the antigen-specific IgA concentrations in fecal samples were significantly higher in group A than in group B at week 2 after inoculation. A large difference between the groups in the number of antigen-specific IgA-secreting cells in the small intestine was observed by immunohistochemical study. Also, the splenic lymphocyte proliferative responses were significantly greater in group A than in the control mice. These results suggest that vaccination with our Salmonella ghost cells can induce both humoral and cell-mediated immune responses and that the increased number of antigen-specific IgA-secreting cells in the small intestine may be correlated with the elevated fecal IgA immune response.     

Hydroxycinnamoylmalic acids and their methyl esters from pear (Pyrus pyrifolia Nakai) fruit peel

Two novel caffeoylmalic acid methyl esters, 2-O-(trans-caffeoyl)malic acid 1-methyl ester (6) and 2-O-(trans-caffeoyl)malic acid 4-methyl ester (7), were isolated from pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) fruit peels. In addition, 5 known hydroxycinnamoylmalic acids and their methyl esters were identified: 2-O-(trans-coumaroyl)malic acid (1), 2-O-(cis-coumaroyl)malic acid (2), 2-O-(cis-coumaroyl)malic acid 1-methyl ester (3), 2-O-(trans-coumaroyl)malic acid 1-methyl ester (4), and 2-O-(trans-caffeoyl)malic acid (phaselic acid, 5). The chemical structures of these compounds were determined by spectroscopic data from ESI MS and NMR. Of all the isolated compounds, five hydroxycinnamoylmalic acids and their methyl esters (2-4, 6, 7) were identified in the pear for the first time.