Pigments in avocado tissue and oil

Pigments are important contributors to the appearance and healthful properties of both avocado fruits and the oils extracted from these fruits. This study determined carotenoid and chlorophyll pigment concentrations in the skin and three sections of the flesh (outer dark green, middle pale green, and inner yellow flesh-nearest the seed) and anthocyanin concentrations in the skin of Hass avocado during ripening at 20 degrees C. Pigments were extracted from frozen tissue with acetone and measured using high-performance liquid chromatography. Pigments were also measured in the oil extracted from freeze-dried tissue sections by an accelerated solvent extraction system using hexane. Carotenoids and chlorophylls identified in the skin, flesh, and oil were lutein, alpha-carotene, beta-carotene, neoxanthin, violaxanthin, zeaxanthin, antheraxanthin, chlorophylls a and b, and pheophytins a and b with the highest concentrations of all pigments in the skin. Chlorophyllides a and b were identified in the skin and flesh tissues only. As the fruit ripened and softened, the skin changed from green to purple/black, corresponding to changes in skin hue angle, and a concomitant increase in cyanidin 3-O-glucoside and the loss of chlorophyllide a. In flesh tissue, chroma and lightness values decreased with ripening, with no changes in hue angle. The levels of carotenoids and chlorophylls did not change significantly during ripening. As fruit ripened, the total chlorophyll level in the oil from the flesh sections remained constant but declined in the oil extracted from the skin.     

Antifungal and insect antifeedant 2-phenylethanol esters from the liverwort Balantiopsis cancellata from Chile

A chemical study of a dichloromethane extract of Balantiopsis cancellata led to the isolation of four known 2-phenylethanol esters (1-4) and a phenylethanediol benzoate (5). Antifeedant activity toward Spodoptera littoralis (disk-choice bioassay) and growth inhibition of the phytopathogen Cladosporium herbarum in TLC-bioautography assays were determined. The results show that the antifeedant and antifungal activity of the extract is attributable mainly to the trans-beta-methylthioacrylate 4.     

Cytoplasmic and nuclear genetic components of membrane stability of winter wheat plants exposed to sub‐zero temperatures

Winter wheat (Triticum aestivum L.) is seeded in the autumn and harvested the following summer, and therefore, must survive multiple episodes of subfreezing temperatures throughout the winter months. Cellular membrane stability following exposure to subfreezing temperatures contributes to the ability to survive these episodes. This study investigated the inheritance of the ability to tolerate subfreezing temperatures with a seven‐parent diallel cross analysis of cellular membrane integrity as measured by electrolyte leakage after exposure to ?10 or ?14 °C. Significant differences in membrane stability were found among the parent lines. The inheritance of the freezing tolerance as measured by electrolyte leakage was complex and characterized by significant additive, dominant and cytoplasmic effects. General combining ability, indicative of additive genetic effects, were significant at both test temperatures, but accounted for 25.5% of the variance at the ?10° C test temperature, and only 4% of the variance at the ?14 °C test temperature. Specific combining ability, indicative of genetic dominance effects, were significant at both test temperatures, but accounted for only 14.6% of the variance at the ?10 °C test temperature, and 38% of the variance at the ?14 °C test temperature. Reciprocal (cytoplasmic) effects were significant and accounted for about 20% of the variance at both test temperatures. Cytoplasmic effects contributing to greater membrane stability were especially apparent in the cultivar Tiber when crossed to Masami, Lewjain, or Hatton. These results suggest that efforts to improve freezing tolerance are complicated by differing gene action at different test temperatures and also may benefit from identifying specific combinations of nuclear and cytoplasm sources that are most conducive to membrane stability following freezing.     

The effect of dietary protein level on pond water quality and feed utilization efficiency of pacúPiaractus mesopotamicus (Holmberg, 1887)

The effect of supplemental feeds with different levels of crude protein on pond water quality and food utilization efficiency by pacú (Piaractus mesopotamicus) was evaluated in a semi‐intensive culture system. Fish were stocked at a density of one individual per m², raised for 299 days, and fed isocaloric diets containing soybean meal, blood and bone meal, and yellow maize. Dietary treatments consisted of diets containing 25%, 35%, and 45% crude protein. Ponds receiving the two highest protein levels showed significantly higher values of alkalinity, conductivity and nitrites. High dietary protein diets resulted in no significant improvements in final weight (336.4±77.2, 308.2±92.6 and 368.4±82.2 g, mean±standard deviation, for 25%, 35% and 45% protein levels respectively). Moreover, fish carcass composition was not significantly different among treatments. Instead, significant augmentations in feed conversion ratio (1.36, 1.54 and 1.73 respectively) and reductions in protein retention (35.7%, 33.5% and 29.0% respectively) occurred as protein levels increased. Results indicate that at the lowest protein level, pacú growth rates and carcass composition were similar to the other experimental treatments, with a significant improvement in pond water quality and feed utilization efficiency.     

The effect of dietary supplementation with globin and spray-dried porcine plasma on performance,digestibility and histomorphological traits in broiler chickens

This study evaluated the effects of globin and spray-dried porcine plasma (SDPP) on growth performance, digestibility, nitrogen retention, energy retention efficiency (ERE) and intestinal morphology of broiler chickens. A total of 336-day-old male broiler chickens were reared from 1 to 40 days of age and fed 3 diets (8 replicates/diet, 14 birds/replicate) during 3 feeding phases: starter (1–12 days), grower (12–25 days) and finisher (25–40 days). Isonitrogenous diets were formulated by replacing gluten protein isolate contained in the control diet (C diet) with 2% (starter) or 1% (grower and finisher) spray-dried porcine plasma in the plasma diet (SDPP diet). The globin diet (G diet) was obtained by adding globin on the top of C diet at a dose of 0.08% for the whole rearing period. Total tract apparent digestibility (aD), nitrogen retention and ERE were assessed during the three growing phases. At 12  and 40 days of age, one bird per pen was slaughtered to sample gut, liver, spleen and bursa of Fabricius for histomorphological investigations. The SDPP diet increased body weights of chickens at 12 (+60 g; p < .001), 25 (+101 g; p < .001) and 40 days (+130 g; p = .018) of age compared to C and G diets. Also SDPP improved crude protein aD (+9.7%) and ERE (+12.3%) during the starter phase (p < .001). Dietary globin and SDPP inclusion did not affect either the gut morphology or the histopathological findings in birds at 12 and 40 days of age, despite a numerical (+6.90% and +7.40% respectively) villus height improvement in the SDPP group. Overall, these results confirm that dietary supplementation with SDPP and, to a lesser extent, with globin can improve growth performance and dietary protein and energy utilization in broiler chickens without effect on gut functionality.     

Latitudinal variability in the seroprevalence of antibodies against Toxoplasma gondii in non-migrant and Arctic migratory geese

Toxoplasma gondii is an intracellular coccidian parasite found worldwide and is known to infect virtually all warm-blooded animals. It requires a cat (family Felidae) to complete its full life cycle. Despite the absence of wild felids on the Arctic archipelago of Svalbard, T. gondii has been found in resident predators such as the arctic fox and polar bear. It has therefore been suggested that T. gondii may enter this ecosystem via migratory birds. The objective of this study was to identify locations where goose populations may become infected with T. gondii, and to investigate the dynamics of T. gondii specific antibodies. Single blood samples of both adults and juveniles were collected from selected goose species (Anser anser, A. brachyrhynchus, Branta canadensis, B. leucopsis) at Arctic brood-rearing areas in Russia and on Svalbard, and temperate wintering grounds in the Netherlands and Denmark (migratory populations) as well as temperate brood-rearing grounds (the Netherlands, non-migratory populations). A modified agglutination test was used on serum, for detection of antibodies against T. gondii. Occasional repeated annual sampling of individual adults was performed to determine the antibody dynamics. Adults were found seropositive at all locations (Arctic and temperate, brood-rearing and wintering grounds) with low seroprevalence in brood-rearing birds on temperate grounds. As no juvenile geese were found seropositive at any brood-rearing location, but nine month old geese were found seropositive during spring migration we conclude that geese, irrespective of species and migration, encounter T. gondii infection in wintering areas. In re-sampled birds on Svalbard significant seroreversion was observed, with 42% of seropositive adults showing no detectable antibodies after 12 months, while the proportion of seroconversion was only 3%. Modelled variation of seroprevalence with field data on antibody longevity and parasite transmission suggests seroprevalence of a population within a range of 5.2–19.9%, in line with measured values. The high occurrence of seroreversion compared to the low occurrence of seroconversion hampers analysis of species- or site-specific patterns, but explains the absence of an increase in seroprevalence with age and the observed variation in antibody titre. These findings imply that even though infection rate is low, adults introduce T. gondii to the high Arctic ecosystem following infection in temperate regions.     

Environmental conditions encountered during typical consumer retail display affect fruit and vegetable quality and waste

Temperature and relative humidity (RH) are the most important environmental factors affecting the sensory quality of fresh produce, and therefore, the consumer acceptability for fruits and vegetables displayed in a produce department. Poor temperature management inevitably occurs in commercial handling and reduces the quality and maximum potential shelf life of fruits and vegetables. Since there is a lack of information on the actual temperatures registered inside local distribution trucks or in consumer retail displays, and the effects on the produce quality, the current study was designed to evaluate the segment of the distribution chain that comprises the time the produce arrives from the distribution center to the store, is displayed at the store, and then stored under household conditions. Temperature and RH were recorded in three different produce departments, at reception and during a 6-week retail display period. Truck and produce temperatures were collected immediately upon opening of the doors, and display temperatures were monitored inside 27 refrigerated and non-refrigerated retail displays. Visual quality of 37 different produce items was evaluated, and surface or pulp temperatures were measured immediately upon unloading from the delivery truck. Reasons for produce waste were recorded on a daily basis during a 6-week period. Shelf life studies were conducted under simulated household conditions using samples collected at the stores. Results from this study showed that chilling-sensitive commodities were transported under too cold conditions, whereas heat-sensitive commodities were transported under too warm conditions. Visual quality of the produce received at the store ranged from good to poor depending on the fruit or vegetable evaluated, with raspberry receiving the lowest visual quality score in 6.7% of store deliveries, and banana, fresh-cut vegetables, peach and pineapple receiving the best visual quality score in 100% of store deliveries. Temperatures measured inside retail displays showed a wide variation, depending on the store and location inside the display, ranging from ?1.2 °C to 19.2 °C in refrigerated displays and from 7.6 °C to 27.7 °C in non-refrigerated displays. RH ranged from 55.9% to 92.9% in refrigerated displays and from 29.7% to 86.6% in non-refrigerated displays. Poor temperature management was the major cause of produce waste (55%) whereas expired date and mechanical damage accounted for 45% of the produce wasted. Results from this study show that fruits and vegetables are often handled under adverse environmental conditions, resulting in produce with poor quality and shorter shelf life, and increased waste at the retail and consumer levels.