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531.
Javed Iqbal Caroline Metosh-Dickey Ralph J. Portier 《Journal of Soils and Sediments》2007,7(3):153-158
Goal, Scope and Background Temperature and soil moisture content are important environmental variables in bioremediation technologies. Optimizing these
variables in-situ would enhance and maintain remediation of hazardous wastes during cold winter seasons or in cold regions and may lead to
reduced maintenance and/or cost. The effect of elevated temperature and soil moisture on bioremediation efficiency was investigated
using a laboratory mesocosm approach. Selected polycyclic aromatic hydrocarbons (PAHs) and phenols degradation in contaminated
flooded soils, commonly found in Superfund sites situated in coastal plains sediments/soils, were evaluated in the mesocosms.
Material and Methods Four laboratory mesocosm treatments in triplicate simulating in-situ bioremediation of contaminated site soils using an immobilized microbe bioreactor system, i.e., bioplug, were established
to evaluate temperature effects. Elevated temperature treatments of site soils with and without contaminant-specific microorganisms
were established at a temperature of 42±2°C. Similarly, treatment of site soils with and without contaminant-specific microorganisms
were established at an ambient temperature of 21±1°C. Composite samples were analyzed for selected PAHs and chlorinated phenols
to determine rates of mineralization and overall remediation efficiency for different temperature regimes.
Results Mesocosm studies indicated that the high temperature inoculated treatment demonstrated a significant reduction in mean total
PAHs and total phenols with a kinetic rate (KR) of 76±13 ng g−1 d−1 in 49 days (approximately 84% reduction; p<0.01) The KR for low temperature inoculated treatment was 54±1 ng g−1 d−1 in 49 days (approximately 66% reduction; p<0.01). High temperature non-inoculated mesocosms exhibited significant mineralization
of all constituents with KR of 15±6 ng g−1 d−1 (approximately 65% reduction; p<0.01) in 49d compared to 54% reduction for low temperature non-inoculated treatment with
KR of 12±3 ng g−1 d−1 (p=0.1794). Phenol compounds in inoculated treatments were also significantly reduced (65%, p<0.01) at elevated temperatures
compared to ambient (52%, p<0.01).
Discussion Increased bioavailability and desorption were noted for elevated temperature and moisture in the soil laboratory mesocosms
simulating a field in situ remediation protocol. This protocol employing the application of immobilized microflora indicated that in situ systems provide an economical advantage if optimal elevated temperature and moisture are controlled properly. Results also
suggested that temperature and moisture optimization needs to be combined with efficient nutrients delivery systems for impacted
soils/sediments.
Conclusions The study demonstrated that temperature and soil moisture contents are important factors in the success of in-situ bioremediation techniques at hazardous waste sites situated in a coastal zone. Kinetic rates were significantly enhanced
to remediate known recalcitrant compounds (PAHs and phenols) in aged soil.
Recommendations and Perspectives The placement of a preferred microbial consortia such as an immobilized microbial population in an entrained bioreactor, i.e.,
bioplug, can significantly reduce constituents of concern in a timely manner for contaminated soils/sediments. However, frequent
monitoring of the soil temperature, moisture content, nutrient level, and dissolved oxygen is necessary to achieve predictable
kinetic rates of mineralization.
ESS-Submission Editor: Dr. Teresa Cutright (tcutright@uakron.edu) 相似文献
532.
Lucas A Rodrigues Michael O Wellington Jolie Caroline Gonzlez-Vega John K Htoo Andrew G Van Kessel Daniel A Columbus 《Journal of animal science》2021,99(5)
We recently showed that dietary supplementation with key functional amino acids (FAA) improves growth performance and immune status of Salmonella Typhimurium (ST)-challenged pigs. It is not known if ST-challenged pigs will benefit from a longer adaptation period to FAA. The objective of this study was to evaluate the effects of different adaptation periods to diets containing FAA above requirements for growth on performance and immune response of weaned pigs subsequently challenged with ST. A total of 32 mixed-sex weanling pigs (11.6 ± 0.3 kg) were randomly assigned to 1 of 4 dietary treatments, being a basal amino acid (AA) profile fed throughout the experimental period (FAA−) or a functional AA profile (FAA+; Thr, Met, and Trp at 120% of requirements) fed only in the postinoculation (FAA+0), for 1 wk pre- and postinoculation (FAA+1), or throughout the experimental period (FAA+2). After a 14-d adaptation period, pigs were inoculated with ST (2.15 × 109 CFU/mL). Growth performance, body temperature, fecal score, acute-phase proteins, oxidant/antioxidant balance, score for ST shedding in feces and intestinal colonization, and fecal and digesta myeloperoxidase (MPO) were measured pre- and postinoculation. Postinoculation body temperature and fecal score, serum haptoglobin, plasma superoxide dismutase (SOD), malondialdehyde (MDA), and fecal MPO were increased while serum albumin and plasma reduced glutathione (GSH):oxidized glutathione (GSSG) were reduced compared to pre-inoculation (P < 0.05). Average daily gain and G:F were greater in FAA+2 pigs compared to FAA− pigs (P < 0.05). Serum albumin was higher in FAA+2 and FAA+1 compared to FAA+0 and FAA− pigs (P < 0.05) while FAA+2 pigs had lower haptoglobin compared to FAA− (P < 0.05). Plasma SOD was increased and GSH:GSSG was decreased in FAA− pigs compared to the other treatments (P < 0.05). Score for ST shedding in feces was progressively lower from d 1 to 6 regardless of treatment (P < 0.05) and was lower in FAA+2 pigs compared to FAA− and FAA+0 (P < 0.05). Counts of ST in colon digesta were higher in FAA− and FAA+0 pigs compared to FAA+2 (P < 0.05). Fecal and colonic digesta MPO were lower in FAA+2 and FAA+1 pigs compared to FAA− (P < 0.05). These results demonstrate a positive effect of a longer adaptation period to FAA-supplemented diets on performance and immune status of weaned pigs challenged with Salmonella. 相似文献
533.
Caroline A. Sullivan David Bourke Micheline Sheehy Skeffington John A. Finn Stuart Green Stephen Kelly Mike J. Gormally 《Biological conservation》2011,(3):1089-1099
A significant policy objective is the need for protection of biodiversity not just within designated Natura 2000 sites, but also in areas that occur outside of these sites. However there is a lack of information on existing semi-natural habitat cover on EU farms, making it difficult to assess whether targets for halting the loss of agricultural biodiversity are being met. To achieve these targets, reliable yet easy-to-use methods are needed for accurately identifying priority areas for conservation actions and monitoring biodiversity on a large spatial scale.Remote sensing, farmland statistics and species data have been used in some EU countries to create maps to estimate the extent of semi-natural habitat cover but these are acknowledged as being too broad scale. In this study, we examined a method of fine-scale prediction of the spatial coverage of semi-natural habitats in lowland farms. A generalized additive model (GAM) was used to investigate the relationships between landscape and farm management variables and the lowland farmland habitat biodiversity on 32 farms outside of conservation designations, in a region of western Ireland. Semi-natural habitat cover on lowland farms could be predicted with a model using stocking density, soil diversity and river and stream length. It is proposed that this model could be used to predict the coverage of semi-natural habitats on farms in other regions of Ireland with similar land-use and landscape. A similar modelling approach could be adapted for application in other regions of Ireland and across Europe with different landscapes to predict semi-natural habitat coverage. 相似文献
534.
As an important component of organic fertilizers, animal faeces require methods for determining diet effects on their microbial quality to improve nutrient use efficiency in soil and to decrease gaseous greenhouse emissions to the environment. The objectives of the present study were (i) to apply the chloroform fumigation extraction (CFE) method for determining microbial biomass in cattle faeces, (ii) to determine the fungal cell-membrane component ergosterol, and (iii) to measure the cell-wall components fungal glucosamine and bacterial muramic acid as indices for the microbial community structure. Additionally, ergosterol and amino sugar data provide independent control values for the reliability of the microbial biomass range obtained by the CFE method. A variety of extractant solutions were tested for the CFE method to obtain stable extracts and reproducible microbial biomass C and N values, leading to the replacement of the original 0.5 M K2SO4 extractant for 0.05 M CuSO4. The plausibility of the data was assessed in a 28-day incubation study at 25 °C with cattle faeces of one heifer, where microbial biomass C and N were repeatedly measured together with ergosterol. Here, the microbial biomass indices showed dynamic characteristics and possible shifts in the microbial community. In faeces of five different heifers, the mean microbial biomass C/N ratio was 5.6, the mean microbial biomass to organic C ratio was 2.2%, and the mean ergosterol to microbial biomass C ratio was 1.1‰. Ergosterol and amino sugar analysis revealed a significant contribution of fungi, with a percentage of more than 40% to the microbial community. All three methods are expected to be suitable tools for analysing the quality of cattle faeces. 相似文献
535.
536.
Sophia G. Moesgaard Torkel Falk Tom Teerlink Halldóra H. Guðmundsdóttir Sif Sigurðardóttir Caroline E. Rasmussen Lisbeth H. Olsen 《Veterinary journal (London, England : 1997)》2011,189(3):349-352
Elevations in the plasma concentrations of natriuretic peptides correlate with increased severity of myxomatous mitral valve disease (MMVD) in dogs. This study correlates the severity of MMVD with the plasma concentrations of the biomarkers N-terminal fragment of the pro-brain-natriuretic peptide (NT-proBNP) and its second messenger, cyclic guanosine monophosphate (cGMP). Furthermore, the l-arginine:asymmetric dimethylarginine (ADMA) ratio was measured as an index of nitric oxide availability. The study included 75 dogs sub-divided into five groups based on severity of MMVD as assessed by clinical examination and echocardiography.Plasma NT-proBNP and cGMP concentrations increased with increasing valve dysfunction and were significantly elevated in dogs with heart failure. The cGMP:NT-proBNP ratio decreased significantly in dogs with heart failure, suggesting the development of natriuretic peptide resistance. Although the l-arginine:ADMA ratio decreased with increasingly severe MMVD, this was largely due to the older age of the dogs with heart failure. 相似文献
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540.
The phenotypic responses of functional traits in natural populations are driven by genetic diversity and phenotypic plasticity. These two mechanisms enable trees to cope with rapid climate change. We studied two European temperate tree species (sessile oak and European beech), focusing on (i) in situ variations of leaf functional traits (morphological and physiological) along two altitudinal gradients and (ii) the extent to which these variations were under environmental and/or genetic control using a common garden experiment. For all traits, altitudinal trends tended to be highly consistent between species and transects. For both species, leaf mass per area displayed a positive linear correlation with altitude, whereas leaf size was negatively correlated with altitude. We also observed a significant increase in leaf physiological performance with increasing altitude: populations at high altitudes had higher maximum rates of assimilation, stomatal conductance and leaf nitrogen content than those at low altitudes. In the common garden experiment, genetic differentiation between populations accounted for 0-28% of total phenotypic variation. However, only two traits (leaf mass per area and nitrogen content) exhibited a significant cline. The combination of in situ and common garden experiments used here made it possible to demonstrate, for both species, a weaker effect of genetic variation than of variations in natural conditions, suggesting a strong effect of the environment on leaf functional traits. Finally, we demonstrated that intrapopulation variability was systematically higher than interpopulation variability, whatever the functional trait considered, indicating a high potential capacity to adapt to climate change. 相似文献