全文获取类型
收费全文 | 746篇 |
免费 | 30篇 |
专业分类
林业 | 64篇 |
农学 | 21篇 |
基础科学 | 4篇 |
255篇 | |
综合类 | 29篇 |
农作物 | 67篇 |
水产渔业 | 90篇 |
畜牧兽医 | 165篇 |
园艺 | 18篇 |
植物保护 | 63篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 9篇 |
2021年 | 16篇 |
2020年 | 17篇 |
2019年 | 31篇 |
2018年 | 25篇 |
2017年 | 27篇 |
2016年 | 19篇 |
2015年 | 17篇 |
2014年 | 37篇 |
2013年 | 47篇 |
2012年 | 63篇 |
2011年 | 64篇 |
2010年 | 32篇 |
2009年 | 33篇 |
2008年 | 61篇 |
2007年 | 55篇 |
2006年 | 38篇 |
2005年 | 41篇 |
2004年 | 34篇 |
2003年 | 25篇 |
2002年 | 38篇 |
2001年 | 1篇 |
2000年 | 8篇 |
1999年 | 2篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1989年 | 2篇 |
1986年 | 2篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有776条查询结果,搜索用时 15 毫秒
711.
Bioenergetic evaluation of diel vertical migration by bull trout (Salvelinus confluentus) in a thermally stratified reservoir 下载免费PDF全文
Madeleine Eckmann Jason Dunham Edward J. Connor Carmen A. Welch 《Ecology of Freshwater Fish》2018,27(1):30-43
Many species living in deeper lentic ecosystems exhibit daily movements that cycle through the water column, generally referred to as diel vertical migration (DVM). In this study, we applied bioenergetics modelling to evaluate growth as a hypothesis to explain DVM by bull trout (Salvelinus confluentus) in a thermally stratified reservoir (Ross Lake, WA, USA) during the peak of thermal stratification in July and August. Bioenergetics model parameters were derived from observed vertical distributions of temperature, prey and bull trout. Field sampling confirmed that bull trout prey almost exclusively on recently introduced redside shiner (Richardsonius balteatus). Model predictions revealed that deeper (>25 m) DVMs commonly exhibited by bull trout during peak thermal stratification cannot be explained by maximising growth. Survival, another common explanation for DVM, may have influenced bull trout depth use, but observations suggest there may be additional drivers of DVM. We propose these deeper summertime excursions may be partly explained by an alternative hypothesis: the importance of colder water for gametogenesis. In Ross Lake, reliance of bull trout on warm water prey (redside shiner) for consumption and growth poses a potential trade‐off with the need for colder water for gametogenesis. 相似文献
712.
Early assessment of gilthead sea bream (Sparus aurata) spawning dynamics by mini‐broodstocks 下载免费PDF全文
The implementation of any sustainable breeding program requires a maximization of effective size (Ne) to maintain inbreeding rate at minimum levels. However, the control of Ne remains a major challenge for those species whose reproduction in captivity is based on a spawning strategy where there is no control over the established matings. The present study aims to assess the spawning dynamic of gilthead sea bream (Sparus aurata) at different dates from two mini‐broodstocks (4 ♀ and 4 ♂). Here we report the use of a simple protocol based on the genotyping of fertilized eggs from a reduced set of microsatellite loci which provides a quick evaluation of daily spawning in terms of family structuring (number of active breeders and number and family size). According to our results, the daily spawning dynamics is characterized by a fluctuation in the number of active breeders, reaching maximum values (until 100%) during peak production phase. However, the proportion of possible mating (families) in a single daily spawning never exceeded more than 81%. Unbalanced parental contributions were highly frequent, with extreme cases involving the assignment of 50%–90% of the offspring to a single family. Therefore, the use of mini‐broodstocks and the grouping of spawning harvested at different dates are proposed as strategies to minimize the inbreeding risk by providing a more optimal pattern of family structuring (increase in the number of families obtained and a more balanced parental contributions) that entails a more conservative Ne/N ratio. 相似文献
713.
714.
Mónica García-López Ricardo I. Pérez-Martín Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(1):114-121
ABSTRACTCarotenoid composition of two commonly discarded decapod crustaceans, Liocarcinus depurator and Munida spp., was assessed in different catching months. Total carotenoid content ranged between 7 ± 1 and 22.4 ± 0.7 μg g?1. Pigment separation was performed by high-performance liquid chromatography coupled with a diode array detector. Astaxanthin accounted for 21 to 50% of total carotenoids, and the remaining percentage corresponded mainly to esters of astaxanthin. The fatty acid profile of carotenoid extracts for each species was evaluated. Overall total unsaturated fatty acids content ranged from 406 to 1,631 μg g?1. Both species were rich in omega-3 fatty acids, which on average represented 68% of total unsaturated fatty acids. Therefore, the use of these low value by-catch species as a source of carotenoid pigments and fatty acids (e.g., for aquaculture formulations) is a possible alternative to their disposal at sea. 相似文献
715.
Jennifer J Swenson Bruce E Young Stephan Beck Pat Comer Jesús H Córdova Jessica Dyson Dirk Embert Filomeno Encarnación Wanderley Ferreira Irma Franke Dennis Grossman Pilar Hernandez Sebastian K Herzog Carmen Josse Gonzalo Navarro Víctor Pacheco Bruce A Stein Martín Timaná Antonio Tovar Carolina Tovar Julieta Vargas Carlos M Zambrana-Torrelio 《BMC ecology》2012,12(1):1-19
Background
The Andes-Amazon basin of Peru and Bolivia is one of the most data-poor, biologically rich, and rapidly changing areas of the world. Conservation scientists agree that this area hosts extremely high endemism, perhaps the highest in the world, yet we know little about the geographic distributions of these species and ecosystems within country boundaries. To address this need, we have developed conservation data on endemic biodiversity (~800 species of birds, mammals, amphibians, and plants) and terrestrial ecological systems (~90; groups of vegetation communities resulting from the action of ecological processes, substrates, and/or environmental gradients) with which we conduct a fine scale conservation prioritization across the Amazon watershed of Peru and Bolivia. We modelled the geographic distributions of 435 endemic plants and all 347 endemic vertebrate species, from existing museum and herbaria specimens at a regional conservation practitioner's scale (1:250,000-1:1,000,000), based on the best available tools and geographic data. We mapped ecological systems, endemic species concentrations, and irreplaceable areas with respect to national level protected areas.Results
We found that sizes of endemic species distributions ranged widely (< 20 km2 to > 200,000 km2) across the study area. Bird and mammal endemic species richness was greatest within a narrow 2500-3000 m elevation band along the length of the Andes Mountains. Endemic amphibian richness was highest at 1000-1500 m elevation and concentrated in the southern half of the study area. Geographical distribution of plant endemism was highly taxon-dependent. Irreplaceable areas, defined as locations with the highest number of species with narrow ranges, overlapped slightly with areas of high endemism, yet generally exhibited unique patterns across the study area by species group. We found that many endemic species and ecological systems are lacking national-level protection; a third of endemic species have distributions completely outside of national protected areas. Protected areas cover only 20% of areas of high endemism and 20% of irreplaceable areas. Almost 40% of the 91 ecological systems are in serious need of protection (= < 2% of their ranges protected).Conclusions
We identify for the first time, areas of high endemic species concentrations and high irreplaceability that have only been roughly indicated in the past at the continental scale. We conclude that new complementary protected areas are needed to safeguard these endemics and ecosystems. An expansion in protected areas will be challenged by geographically isolated micro-endemics, varied endemic patterns among taxa, increasing deforestation, resource extraction, and changes in climate. Relying on pre-existing collections, publically accessible datasets and tools, this working framework is exportable to other regions plagued by incomplete conservation data. 相似文献716.
Olga Alvarez Colom Isabel Barrachina Ildefonso Ayala Mingol M. Carmen Gonzalez Mas Pilar Moya Sanz Adriana Neske Alicia Bardon 《Journal of pest science》2008,81(2):85-89
From an Argentine collection of Annona cherimolia and a Bolivian collection of Annona montana (Annonaceae), the acetogenins, squamocin, molvizarin, itrabin, almuñequin, cherimolin-1, annonacin, annonacin-A, densicomacin-1, cis-annonacin-10-one and murihexocin-A, were obtained to study their toxicity against the cotton pest Oncopeltus fasciatus (Hemiptera: Lygaeidae). Topical ventral application of O. fasciatus nymphs with solutions of the mentioned acetogenins produced acute and delayed mortality. 相似文献
717.
Irene Romero María T. Sanchez-Ballesta María I. Escribano Carmen Merodio 《Postharvest Biology and Technology》2008,49(1):1-9
In this study, we have analyzed the profiles of individual anthocyanins in the skin of Cardinal table grapes and their contribution to the total antioxidant capacity (TAC) in response to low temperature (0 °C) and high CO2 levels (20% for 3 days). An analysis of the representative colour parameters of this red-violet variety was also determined. The anthocyanin composition was determined using high-performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC–DAD–MS). The contribution of individual anthocyanins to the TAC value of table grapes was calculated on the basis of their concentration and antioxidant capacity measured as the TEAC value (slope of the anthocyanin/slope of Trolox). Chromatographic analysis identified six anthocyanins, including pelargonidin 3-glucoside (Pg-3-G), in the skin of Cardinal table grapes. Short-term storage at 0 °C in air had an increasing effect on the concentration of each of the identified anthocyanins. After 3 days at 0 °C, untreated grapes had the highest anthocyanin content (27.55 mg/100 g FW) and displayed the largest TAC value (52.45 mM TE/100 g FW). Peonidin 3-glucoside (Pn-3-G) was the predominant anthocyanin, and it was mainly responsible for the rise in the calculated TAC value in untreated grapes. Pn-3-G had a low average TEAC value (1.73 mM), but its contribution could be explained by the sharp increase in the content of this anthocyanin the first days of storage at 0 °C. In contrast, the Pn-3-G content in grapes at the end of the 3-day CO2 treatment did not change, and both the total anthocyanin content and the calculated TAC value remained significantly constant in comparison to the levels in pre-stored grapes. In addition CO2 treatment had a positive effect on the amount of Pg-3-G concomitant with a pronounced decline in delphinidin 3-glucoside (Dp-3-G) and smaller decreases in petunidin 3-glucoside (Pt-3-G) and malvidin 3-glucoside (Mv-3-G). These effects of high CO2 levels on the profile of individual anthocyanins were progressively lost when grapes were transferred to air. Indeed, after prolonged low-temperature storage when the colour of the berries become dark violet, similar calculated TAC values were quantified in untreated grapes and in those exposed to 20% CO2. These data provide new insights about the effect of low temperature and high CO2 levels on the concentration of the individual anthocyanins identified in cardinal table grapes. 相似文献
718.
Geographic variation in tree growth and wood density of <Emphasis Type="Italic">Guazuma crinita</Emphasis> Mart. in the Peruvian Amazon 总被引:1,自引:0,他引:1
Guazuma crinita Mart. is a fast-growing timber tree in the Peruvian Amazon. A trial of 11 Peruvian provenances was established in three planting
zones. Tree growth at 6, 12, 18 and 30 months and wood density at 32 months were analyzed across and within zones. Zones accounted
for more variation than provenances. Density varied significantly due to provenances in the analysis across zones, whereas
growth exhibited strong provenance by zone interactions. The local provenance grew well and produced relatively dense wood
in all zones. The relative magnitude of variation due to provenances depended on the planting zone. Density was greater in
the lower than in the upper stem. Density increased with longitude from the more humid to the drier part of the sample region.
In general, correlations indicated that larger trees had lower density and a larger difference in density between the lower
and upper stem, but the strength of these relationships depended on the provenance and zone. Results suggest that fast-growing
provenances can be selected at an early age without significantly reducing wood density. The local provenance is recommended
for reforestation pending future research demonstrating the superiority of foreign provenances. Some practical implications
for tree-improvement programs are discussed. 相似文献
719.
Pedrero Z Madrid Y Hartikainen H Cámara C 《Journal of agricultural and food chemistry》2008,56(1):266-271
The protective effect of selenium against the cadmium-induced oxidative effect in broccoli ( Brassica oleracea) plants was studied. Plants grown in hydroponic culture were supplied with selenium [as Se(IV)] and cadmium [as Cd(II)], individually or simultaneously. Cadmium accumulation in roots was noticeably higher than in the aerial parts of the plants, and this effect was even more acute when selenium was simultaneously added. Cadmium phytotoxicity was evidenced by an increase in the malondialdehyde (MDA) concentration in the roots and a decrease of photosynthetic pigment and tocopherol concentration in the aerial parts of the plant. The simultaneous addition of selenium alleviated cadmium-induced stress in the roots after 40 days of exposition. In the leaves, a more remarkable decrease of tocopherol and chlorophyll concentration was observed in the cadmium-enriched plants after 10 days of exposure. The results provided evidence that selenium supplementation helps the plant to minimize the cadmium oxidant effect. Tocopherol concentration in broccoli fruit of cadmium-supplied plants was not affected in comparison to control. However, the proportion of alpha-tocopherol increases with the addition of selenium. This response is important not only for the protective effect against oxidative damage in the plant but also in terms of human nutrition. 相似文献
720.
Domínguez MJ Sanmartín C Font M Palop JA San Francisco S Urrutia O Houdusse F García-Mina J 《Journal of agricultural and food chemistry》2008,56(10):3721-3731
The design, synthesis, and biological evaluation of phosphoramide derivatives as urease inhibitors to reduce the loss of ammonia has been carried out. Forty phosphorus derivatives were synthesized and their inhibitory activities evaluated against that of jack bean urease. In addition, in vivo assays have been carried out. All of the compounds were characterized by IR, (1)H NMR, MS, and elemental microanalysis. In some cases, detailed molecular modeling studies were carried out, and these highlighted the interaction between the enzyme active center and the compounds and also the characteristics related to their activity as urease inhibitors. According to the IC(50) values for in vitro inhibitory activity, 12 compounds showed values below 1 microM and 8 of them represent improvements of activity in comparison to the commercial urease inhibitor N-n-butylthiophosphorictriamide (NBPT) (100 nM) (AGROTAIN). On the basis of the activity results and the conclusions of the molecular modeling study, a structural model for new potential inhibitors has been defined. 相似文献