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21.
Lourdes Cardozo Tllez Alice Chavez Nathalia Bobadilla Pastor Prez‐Estigarribia Mohan Kohli 《Plant Breeding》2019,138(1):62-68
Wheat blast disease, caused by Magnaporthe oryzae (anamorph Pyricularia oryzae), produces severe damage to wheat production in South America. It was observed that many resistant cultivars contain the 2NS/2AS translocation from Triticum ventricosum. In this study, we evaluate the presence of the 2NS/2AS translocation in 57 advanced breeding lines and one variety ‘Caninde 1’ from Paraguayan wheat germplasm, using VENTRIUP‐LN2 primers. The germplasm ‘Caninde 1 and 22’ of the breeding lines, found positive for the presence of 2NS/2AS translocation, were inoculated with a single aggressive Magnaporthe pathotype P14‐039, to assess their response to wheat blast infection under controlled conditions. Based on the disease infection score, ten of the breeding lines, ‘Caninde 1’ and ‘Milan’ (positive control), were classified as resistant. Three of the remaining breeding lines were classified as moderately resistant, five as moderately susceptible and other four as susceptible. Our results show that the expression of 2NS/2AS‐based blast resistance is more dependent on genetic background of the inserted germplasm than previously envisioned. 相似文献
22.
Larissa B Cardozo DVM Ricardo M Almeida† DVM PhD Diplomate CBCAV Levi C Fiúza‡ DVM & Paula D Galera DVM PhD 《Veterinary anaesthesia and analgesia》2009,36(4):396-400
Objective To evaluate the quality of brachial plexus blockade with 0.75% ropivacaine in domestic chickens.
Study design Prospective experimental trial.
Animals Six 30-week-old female chickens, weighing 4.5 ± 0.4 kg.
Methods Six brachial plexus injections were performed after anesthetic induction with isoflurane. After achieving adequate muscle relaxation, the animals were positioned in dorsal recumbency and injected with ropivacaine (1 mL kg−1 ). The birds recovered and assessments of motor function and response to pinch were scored every 5 minutes for 180 minutes. The scores were from zero (no response) to three (greatest response). The scores over time were analyzed using a Wilcoxon nonparametric test with statistical significance accepted if p ≤ 0.05.
Results There was a significant difference ( p < 0.05) from 15 to 130 minutes and 15 to 120 minutes for motor and sensory blocks, respectively. The onset of both blocks took 15 minutes and the effective periods of sensory and motor anesthesia were 105 and 115 minutes, respectively. Comparison between blocks at different times did not demonstrate significant differences ( p > 0.05).
Conclusions and clinical relevance No complications were observed after the technique. Brachial plexus blockade with 0.75% ropivacaine is a simple and effective technique for procedures on the thoracic limb of domestic chickens. 相似文献
Study design Prospective experimental trial.
Animals Six 30-week-old female chickens, weighing 4.5 ± 0.4 kg.
Methods Six brachial plexus injections were performed after anesthetic induction with isoflurane. After achieving adequate muscle relaxation, the animals were positioned in dorsal recumbency and injected with ropivacaine (1 mL kg
Results There was a significant difference ( p < 0.05) from 15 to 130 minutes and 15 to 120 minutes for motor and sensory blocks, respectively. The onset of both blocks took 15 minutes and the effective periods of sensory and motor anesthesia were 105 and 115 minutes, respectively. Comparison between blocks at different times did not demonstrate significant differences ( p > 0.05).
Conclusions and clinical relevance No complications were observed after the technique. Brachial plexus blockade with 0.75% ropivacaine is a simple and effective technique for procedures on the thoracic limb of domestic chickens. 相似文献