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101.
102.
微量补体结合试验检测牛、羊副结核病的研究 总被引:1,自引:0,他引:1
采用《中华人民共和国出入境检验检疫行业标准》“副结核病补体结合试验操作规程”方法(简称常量法)和微量补体结合试验(简称微量法)检测3677头进口牛羊的副结核病抗体并进行比较。结果表明,常量法和微量法的溶血素效价测定值相同,补体效价测定值近似,阴性血清对照、抗原对照、补体对照、红细胞对照、血清抗补体结合对照结果均相同,阳性对照血清效价微量法比常量法提高1个滴度,被检血清的阳性检出率微量法高于常量法。比较37℃水浴及37℃恒温培养箱环境下的微量法补体结合试验结果,溶血素效价、补体效价、各项对照试验结果完全相同,被检血清在37℃水浴反应更加充分。比较用变态试验和补体结合试验检验3677头牛羊副结核病的结果、以及用补体结合试验、变态反应和酶联免疫吸附试验方法同时检测2024头牛的结果,发现它们的相关性很小。 相似文献
103.
Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants 总被引:14,自引:0,他引:14
Cao J Henry PR Guo R Holwerda RA Toth JP Littell RC Miles RD Ammerman CB 《Journal of animal science》2000,78(8):2039-2054
Eight commercially available organic Zn products and reagent-grade ZnSO4 x 7H2O (Zn Sulf) were evaluated by polarographic analysis, and solubility in .1 M K2HPO4-KH2PO4 buffer (pH 5), .2 M HCl-KCl buffer (pH 2), and deionized water. Fractions from these solubility tests were evaluated by gel filtration chromatography for structural integrity. Degree of chelation was generally positively related to chelation effectiveness determined by polarography. The organic sources were Zn methionine complex A (Zn MetA), Zn methionine complex B (Zn MetB), Zn polysaccharide complex (Zn Poly), Zn lysine complex (Zn Lys), Zn amino acid chelate (Zn AA), Zn proteinate A (Zn ProA), Zn proteinate B (Zn ProB), and Zn proteinate C (Zn ProC). Three experiments were conducted to estimate the relative bioavailability of Zn from the organic Zn supplements for chicks and lambs when added at high dietary levels to practical diets. Bone Zn concentration increased (P < .001) as dietary Zn increased in both experiments. When Zn Sulf was assigned a value of 100% as the standard, multiple linear regression slope ratios of bone Zn from chicks fed 3 wk regressed on dietary Zn intake gave estimated relative bioavailability values of 83 +/- 14.6 and 139 +/- 16.9 for Zn AA and Zn ProA, respectively, in Exp. 1 and 94 +/- 11.6, 99 +/- 8.8, and 108 +/- 11.4 for Zn Poly, Zn ProB, and Zn ProC, respectively, in Exp. 2. In Exp. 3, 42 lambs were fed diets containing Zn Sulf, Zn ProA, Zn AA, or Zn MetB for 21 d. Based on multiple linear regression slope ratios of liver, kidney, and pancreas Zn and liver metallothionein concentrations on added dietary Zn, bioavailability estimates relative to 100% for Zn Sulf were 130, 110, and 113 for Zn ProA, Zn AA, and Zn MetB, respectively. Except for Zn ProA, which was greater, the organic Zn supplements had bioavailability values similar to that of Zn Sulf for chicks and lambs. Bioavailability of organic Zn products was inversely related to solubility of Zn in pH 5 buffer in chicks (r2 = .91) and pH 2 buffer in lambs (r2 = .91), but not to an estimate of degree of chelation. 相似文献
104.
霉菌毒素对家禽生产的影响 总被引:2,自引:0,他引:2
<正>霉菌毒素是霉菌以饲料为营养源的生长过程中所产生的有毒化学物质。在谷物收割前或贮存、运输、加工和饲喂过程中,霉菌都可在其中生长并产生霉菌毒素。全球每年大约有25%的农作 相似文献
105.
Background:The experiment evaluated the effect of nutrition levels and sex on the growth performance,carcass characteristics and meat quality of F1 Angus × Chinese Xiangxi yellow cattle.Methods:During the background period of 184 d,23 steers and 24 heifers were fed the same ration,then put into a2×2×2 factorial arrangement under two levels of- dietary energy(TON:70/80%DM),protein(CP:11.9/14.3%DM)and sex(S:male/female) during the finishing phase of 146 d.The treatments were-(1) high energy/low protein(HELP),(2) high energy/high protein(HEHP),(3) low energy/low protein(LELP) and(4) low energy/high protein(LEHP).Each treatment used 6 steers and 6 heifers,except for HELP- 5 steers and 6 heifers.Results:Growth rate and final carcass weight were unaffected by dietary energy and protein levels or by sex.Compared with the LE diet group,the HE group had significantly lower dry matter intake(DMI,6.76 vs.7.48 kg DM/d),greater chest girth increments(46.1 vs.36.8 cm),higher carcass fat(19.9 vs.16.3%) and intramuscular fat content(29.9 vs.22.8%DM).The HE group also had improved yields of top and medium top grade commercial meat cuts(39.9 vs.36.5%).The dressing percentage was higher for the HP group than the LP group(53.4 vs.54.9%).Steers had a greater length increment(9.0 vs.8.3 cm),but lower carcass fat content(16.8 vs.19.4%) than heifers.The meat quality traits(shear force value,drip loss,cooking loss and water holding capacity) were not affected by treatments or sex,averaging 3.14 kg,2.5,31.5 and 52.9%,respectively.The nutritive profiles(both fatty and amino acid composition) were not influenced by the energy or protein levels or by sex.Conclusions:The dietary energy and protein levels and sex significantly influenced the carcass characteristics and chemical composition of meat but not thegrowth performance,meat quality traits and nutritive profiles. 相似文献
106.
107.
近年来,高致病性禽流感、高致病性猪蓝耳病等重大动物疫病的发生,折射出了动物卫生监督机构在重大动物疫病防疫监督工作中尚存不足。为此,笔者结合当前重大动物疫病防疫 相似文献
108.
109.
为探究鱼菜共生系统中适宜氮元素高效循环利用的鱼粪生化处理工艺,设置厌氧和好氧两种工艺对鱼粪进行生化处理,每种工艺设置三个反应温度,三个鱼粪TS浓度,研究不同工艺处理后鱼粪液中氮元素变化规律以及鱼粪液对生菜生长的影响。结果表明,经厌氧处理后的鱼粪液铵硝比增大,经好氧处理后的鱼粪液铵硝比降至1.0以下,TS浓度3.0-3.5%的鱼粪经35.0℃好氧处理后水培生菜的生长速率最优,说明最适宜生菜生长的鱼粪生化处理工艺为:原鱼粪TS浓度3.0-3.5%,经35.0℃好氧工艺处理10天。 相似文献
110.
低比转数离心泵叶轮出口紊流流动结构分析 总被引:4,自引:1,他引:4
在叶轮内三维紊流流动数值计算的基础上,从介质能量的观点,分析了不同工况下低比转数离心泵叶轮出口处的紊流流动结构。数值计算采用SIMPLEC算法,在贴体坐标系下求解Navier—Stokes方程,紊流模型采用标准的κ—ε模型。计算结果表明,在叶轮的出口附近靠近吸力面一侧存在一个低能区,而压力面一侧能量相对较高。这与有关文献中所指出的离心风机的“射流—尾流”结构相对应。当偏离设计工况时,该低能区的范围和强度略有增加,相对应的叶轮效率也降低。计算结果与水泵在这些工况下的效率实验结果相一致,说明低能区的存在是影响低比转数离心泵效率的重要因素。 相似文献