ALLEVIATION OF ALUMINUM IN ACIDIC SOILS AND ITS EFFECT ON GROWTH OF HYBRID AND CLONAL OIL PALM SEEDLINGS

Aluminum (Al) toxicity is an important factor in limiting crop production. The present study examined the Al alleviation effects on the growth of hybrid (breeding) and clonal (tissue culture) of D × P oil palm seedlings. The experiment was performed using calcium carbonate (CaCO₃), and ground magnesium limestone (GML) and magnesium carbonate (MgCO₃) as soil-amendments at different rates in Colombia and Malaysia, respectively. The effects of the treatments were evaluated monthly on vegetative variables and visual symptoms. Chlorophyll concentrations were recorded in Malaysia at the fifth month growing stage. The different amendments improved the soil fertility and it was reflected on better performance of shoot and root growth. The chlorophyll content in the frond number 3 for both materials enhanced significantly when Al saturation was low (0–30%). The results from the experiment revealed the importance of neutralization of Al in reducing its toxicity in oil palm.     

Soil organic carbon stocks in Veracruz State (Mexico) estimated using the 1:250,000 soil database of INEGI: biophysical contributions

Purpose

In this study, we quantified soil organic carbon (SOC) stocks and analyzed their relationship with biophysical factors and soil properties.

Materials and methods

The study region was Veracruz State, located in the eastern part of Mexico, covering an area of 72,410 km². A soil database that contains physicochemical analyses of soil horizons such as carbon concentration data was the source of information used in this study. The database consisted of 163 soil profiles representing 464 genetic horizons. Statistical analysis was used to investigate the effect of each factor (climate, altitude, slope) on SOC stock to 0.50 m depth and to assess differences in the distribution of SOC stock in terms of soil depth (0.0–0.20, 0.20–0.40, 0.40–0.60, 0.60–0.80, 0.80–1.0 m) and land use. In order to compute the spatial distribution of SOC stock to 0.50 m depth based on the soil sampling location, the kriging method was used.

Results and discussion

Results indicated that SOC stock (0.50 m depth) ranged between 0.44 and 41.2 kg C m^?2. Regression analysis showed that SOC stocks (0.50 m depth) are negatively correlated with temperature (r?=??0.38; P?<?0.001) and positively correlated with altitude (r?=?0.40; P?<?0.001) and slope (r?=?0.40; P?<?0.001). In addition, by multiple regression, temperature combined with precipitation explained more SOC stock variations (r?=?0.43; P?<?0.001) than the regression model with precipitation (r?=?0.13; P?=?0.16) alone. Also, slope combined with temperature and precipitation explained more SOC stock variations (r?=?0.46; P?<?0.001) than the regression model with slope alone. Forest lands, grasslands, and croplands have higher SOC stocks in the 0.0–0.20-m soil layer than in deeper layers. On average, forest lands, grasslands, croplands, and other lands (wetland and dunes) had a SOC stock of 13.6, 14.6, 15.1, and 8.5 kg C m^?2 at 1 m depth, respectively. Soil color correlated (?0.25 ≤ r ≤ ?0.89) with SOC content.

Conclusions

Overall, these results indicate the influence of major interactions between biophysical factors and SOC stocks. This research indicated that SOC stock decreased with soil depth, but with slight variations depending on land use. Thus, there remains a need for more SOC data that include an improved distribution of soil sampling points in order to entirely understand the contributions of biophysical factors to SOC stocks in Veracruz State.     

Impact of soil types on sugarcane development monitored over time by remote sensing

Soil is one of the most important factors for agricultural production. In tropical regions, soil variability is considerable, with the most diverse combinations of physical and chemical characteristics, an influence factor in crop growth and productivity. In this research, the main objective was to identify how soil characteristics and parent material can influence sugarcane development over time using remote sensing. An area located in Sao Paulo, Brazil, of 182 ha (one point per ha with soil analysis), with high variability in the parent material and soil types, was selected. Images from the Sentinel2-MSI satellite were used to describe the spectral behavior of sugarcane over a period of one year. The NDRE (normalized difference red-edge index) was calculated for each image and then the leaf area index (LAI) was obtained from it. Maps of soil classes, soil properties at two depths (0–0.20 and 0.80–1.0 m), and parent material classes were related to sugarcane LAI variability over time. Production environment zones, which is a classification based on soil characteristics to support sugarcane development, were also obtained and related to LAI variability. Spectral signatures of the crop presented different behaviors through the season, soil types and soil attributes provided useful responses for this variability. At the beginning of the season, the surface and subsurface soil properties (texture and fertility) impacted differently on crop development. On the other hand, soil classes and parent material influenced LAI in all production environments studied. The results indicated that the soil types and their properties at different depths have a significant impact on sugarcane development. Furthermore, RS was able to monitor the plant evolution and be related to soil types which may assist in plant management. The results can bring light on how better sugarcane management can be conducted using remote sensing data and soils variability.

     

Bovine leptospirosis in abattoirs in Uganda: Molecular detection and risk of exposure among workers

Leptospirosis is a zoonotic bacterial disease reported worldwide. In Uganda, seropositivity has been reported in both humans and domesticated animals, including cattle. However, it remains unknown whether cattle are shedding leptospires and thus acting as potential source for human leptospirosis. We conducted this cross‐sectional study in two cattle abattoirs in Kampala, Uganda between June and July 2017. Kidney and urine samples from 500 cattle sourced from across the country were analysed by real‐time PCR to establish the prevalence of Leptospira‐positive cattle and risk of exposure to abattoir workers. The species of infecting Leptospira was determined by amplification of secY gene and compared to reference sequences published in GenBank. Of 500 cattle tested, 36 (7.2%) had Leptospira DNA in their kidneys (carriers), 29 (5.8%) in their urine (shedders); with an overall prevalence (kidney and/or urine) of 8.8%. Leptospira borgpetersenii was confirmed as the infecting species in three cattle and Leptospira kirschneri in one animal. Male versus female cattle (OR = 3, p‐value 0.003), exotic versus local breeds (OR = 21.3, p‐value 0.002) or cattle from Western Uganda (OR = 4.4, p‐value 0.001) and from regions across the border (OR = 3.3, p‐value 0.032) versus from the central region were more likely to be Leptospira‐positive. The daily risk of exposure of abattoir workers to ≥1 (kidney and/or urine) positive carcass ranged from 27% (95% credibility interval 18.6–52.3) to 100% (95% CI 91.0–100.0), with halal butchers and pluck inspectors being at highest risk. In conclusion, cattle slaughtered at abattoirs in Uganda carry and shed pathogenic Leptospira species; and this may pose occupation‐related risk of exposure among workers in these abattoirs, with workers who handle larger numbers of animals being at higher risk.     

Seroprevalence and risk factors for leptospirosis in cattle,sheep, and goats at consorted rearing from the State of Piauí, northeastern Brazil

Leptospirosis is an endemic disease in Latin America, caused by pathogenic bacteria of the genus Leptospira. It is considered one of the main causes responsible for the negative economic impact on global livestock by causing reproductive problems. The research aimed to determine the prevalence of leptospirosis in cattle, sheep, and goats at consorted rearing in the micro-region of Teresina, Piauí state, northeastern Brazil, as well as to identify prevalent serovars and risk factors associated with seroprevalence. Serum samples were analyzed in 336 sheep, 292 goats, and 253 cattle using microscopic agglutination test (MAT). Overall, 378 samples were positive to MAT, with seroprevalence of 42.9%. The prevalences in cattle, sheep, and goats were 50.5, 40.5, and 34.6%, respectively. All herds presented at least one seropositive animal; the Hardjo/Wolffi serovar association was the most common in cattle and Icterohaemorrhagiae in goats and sheep. Beef production (OR?=?4.9), cattle herd over 35 animals (OR?=?4.0), feeding on pasture (OR?=?6.4), weir and/or stream as water source (OR?=?2.1), and no veterinary services (OR?=?2.9) were risk factors for cattle infection. For sheep, intensive management system (OR?=?5.3), suspended slatted facilities (OR?=?2.2), more than 20 sheep in reproductive age (OR?=?1.9), and absence of deworming (OR?=?3.5) were the risk factors, while for goats, the identified risk factors were sheep herd over 52 animals (OR?=?1.9) and no veterinary services (OR?=?1.8). We conclude that the infection was spreading in consorted herds in this region. Thus, it would be interesting and important to conduct educative activities to farmers on the economic impacts of this disease and the need of preventive and control strategies mainly focused on sanitary measures and animal handling.     

Genetic analysis of growth,visual scores,height, and carcass traits in Nelore cattle

Covariance components were estimated for growth traits (BW, birth weight; WW, weaning weight; YW, yearling weight), visual scores (BQ, breed quality; CS, conformation; MS, muscling; NS, navel; PS, finishing precocity), hip height (HH), and carcass traits (BF, backfat thickness; LMA, longissimus muscle area) measured at yearling. Genetic gains were obtained and validation models on direct and maternal effects for BW and WW were fitted. Genetic correlations of growth traits with CS, PS, MS, and HH ranged from 0.20 ± 0.01 to 0.94 ± 0.01 and were positive and low with NS (0.11 ± 0.01 to 0.20 ± 0.01) and favorable with BQ (0.14 ± 0.02 to 0.37 ± 0.02). Null to moderate genetic correlations were obtained between growth and carcass traits. Genetic gains were positive and significant, except for BW. An increase of 0.76 and 0.72 kg is expected for BW and WW, respectively, per unit increase in estimated breeding value (EBV) for direct effect and an additional 0.74 and 1.43, respectively, kg per unit increase in EBV for the maternal effect. Monitoring genetic gains for HH and NS is relevant to maintain an adequate body size and a navel morphological correction, if necessary. Simultaneous selection for growth, morphological, and carcass traits in line with improve maternal performance is a feasible strategy to increase herd productivity.     

Single‐Layer Centrifugation Reduces Equine Arteritis Virus Titre in the Semen of Shedding Stallions

Several countries have adopted strategies for preventing and/or controlling equine viral arteritis based on vaccination and restricting the breeding activities of carrier stallions. However, in some cases, carrier stallions are only identified after they have transmitted virus to a mare. Therefore, a mechanism for separating virus from spermatozoa in the semen of carrier stallions would facilitate control measures for preventing disease transmission. In this study, the use of several modifications of single‐layer centrifugation (SLC, SLC with an inner tube and double SLC) through Androcoll‐E, a species‐specific colloid were evaluated for their ability to separate spermatozoa from virus in ejaculates from carrier stallions. The three types of SLC significantly reduced the virus titre in fresh semen at 0 h and in stored semen at 24 h (p < 0.001) but did not completely eliminate the virus. Sperm motility parameters such as total motility and progressive motility were significantly increased after colloid centrifugation, whereas curvilinear velocity and amplitude of lateral head deviation were decreased, and the remainder (straight line velocity, average path velocity, straightness, linearity, wobble and beat cross‐frequency) were not significantly affected by the processing. Although virus titres were reduced in the SLC samples, significant levels of infectivity still remained, especially in stallions shedding large amounts of virus. It remains to be determined whether SLC‐processed sperm samples from stallions shedding low virus titres retain sufficient equine arteritis virus to cause infection in mares through artificial insemination.     

Intake and ingestive behavior of lambs fed diets containing ammoniated buffel grass hay

This study aimed to evaluate the effect of diets with ammoniated buffel grass hay on the ingestive behavior of feedlot lambs. Thirty-two sheep of no defined breed with an average body weight of 17.7?±?1.8 kg were used. A completely randomized design with four treatments (0, 18, 36, and 54 g/kg dry matter (DM) basis) and eight repetitions was used. Ingestive behavior, rumination, and idle time were similar (P?>?0.05) among the diets containing ammoniated buffel grass hay, with mean values of 294.5, 554.44, and 594.25 min per day, respectively. Regarding the chews, all of the variables resulted in similar behavior (P?>?0.05). The quadratic effect (P?<?0.05) observed for daily intake can be explained based on the amount of DM and neutral detergent fiber (NDF) per meal per rumination. There was no effect of urea levels from the ammoniation (P?>?0.05) on the efficiency of DM and NDF consumption. However, the rumination efficiency of DM and NDF showed a quadratic effect (P?<?0.05). Thus, the use of ammoniated buffel grass hay with urea in lamb diet affects the ingestive behavior by increasing the rumination efficiency, increased intake, and feed utilization.