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121.
Dynamic instability-the switching of a two-state polymer between phases of steady elongation and rapid shortening-is essential to the cellular function of eukaryotic microtubules, especially during chromosome segregation. Since the discovery of dynamic instability 20 years ago, no other biological polymer has been found to exhibit this behavior. Using total internal reflection fluorescence microscopy and fluorescence resonance energy transfer, we observe that the prokaryotic actin homolog ParM, whose assembly is required for the segregation of large, low-copy number plasmids, displays both dynamic instability and symmetrical, bidirectional polymerization. The dynamic instability of ParM is regulated by adenosine triphosphate (ATP) hydrolysis, and filaments are stabilized by a cap of ATP-bound monomers. ParM is not related to tubulin, so its dynamic instability must have arisen by convergent evolution driven by a set of common constraints on polymer-based segregation of DNA. 相似文献
122.
The exogenic sulfur cycle is tightly coupled with the carbon and oxygen cycles, and therefore a central component of Earth's biogeochemistry. Here we present a high-resolution record of the sulfur isotopic composition of seawater sulfate for the Cretaceous. The general enrichment of isotopically light sulfur that prevailed during the Cretaceous may have been due to increased volcanic and hydrothermal activity. Two excursions toward isotopically lighter sulfur represent periods of lower rates of pyrite burial, implying a shift in the location of organic carbon burial to terrestrial or open-ocean settings. The concurrent changes in seawater sulfur and inorganic carbon isotopic compositions imply short-term variability in atmospheric oxygen partial pressure. 相似文献
123.
A computer program that uses artificial intelligence techniques has successfully identified the location of a porphyry molybdenum deposit. Given geological maps of readily available predrilling exploration data for Mount Tolman in Washington State and using rules obtained from a porphyry molybdenum exploration specialist, the program (called PROSPECTOR) identified the location of previously unknown ore-grade mineralization. This appears to be the first reported determination of the location of mineralization by such a computer-based approach. 相似文献
124.
Ellsworth WL Campbell RH Hill DP Page RA Alewine RW Hanks TC Heaton TH Hileman JA Kanamori H Minster B Whitcomb JH 《Science (New York, N.Y.)》1973,182(4117):1127-1129
Seismological investigations show that the Point Mugu earthquake involved north-south crustal shortening deep within the complex fault zone that marks the southern front of the Transverse Ranges province. This earthquake sequence results from the same stress system responsible for the deformation in this province in the Pliocene through Holocene and draws attention to the significant earthquake hazard that the southern frontal fault system poses to the Los Angeles metropolitan area. 相似文献
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Mora Campbell 《Agriculture and Human Values》1998,15(1):57-64
The metaphor of the food system,dominant in current research approaches to sustainableagriculture, mirrors the productionist paradigm, whichreduces our relationship to land and food to theproduction and consumption of commodities. Theenactment of the familiar values of nourishment andhospitality is what the goal of sustainableagriculture would amount to in terms of our day to daylived experience. The metaphor of an earthen bowl asa theory of food and agriculture can embody thesevalues such that broader change might be achievedthrough embracing the idea and practice of theories asnourishing and theorizing as hospitable. 相似文献
127.
The simian guartan malaria parasite Plasmodium inui (OS strain) was cultured in a continuous flow system with rhesus monkey erythrocytes and RPMI 1640nmedium supplemented with Hepes buffer and rhesus serum. Over a 10-week period, the growth of the parasite permitted a 61,000-fold cumulative dilution of the original inoculum. After 5 weeks in culture, the parasites were still infective to the monkey Saimiri sciureus and to Anopheles freeborni mosquitoes. 相似文献
128.
Atlas R Campbell P Cozzarelli NR Curfman G Enquist L Fink G Flanagin A Fletcher J George E Hammes G Heyman D Inglesby T Kaplan S Kennedy D Krug J Levinson R Marcus E Metzger H Morse SS O'Brien A Onderdonk A Poste G Renault B Rich R Rosengard A Salzburg S Scanlan M Shenk T Tabor H Varmus H Wimmer E Yamamoto K;Journal Editors Authors Group 《Science (New York, N.Y.)》2003,299(5610):1149
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