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61.
Daniela Minerdi Marino Moretti Yuan Li Laura Gaggero Angelo Garibaldi Maria Lodovica Gullino 《European journal of plant pathology / European Foundation for Plant Pathology》2008,122(2):227-237
A conventional PCR and a SYBR Green real-time PCR assays for the detection and quantification of Phytophthora cryptogea, an economically important pathogen, have been developed and tested. A conventional primer set (Cryp1 and Cryp2) was designed
from the Ypt1 gene of P. cryptogea. A 369 bp product was amplified on DNA from 17 isolates of P. cryptogea. No product was amplified on DNA from 34 other Phytophthora spp., water moulds, true fungi and bacteria. In addition, Cryp1/Cryp2 primers were successfully adapted to real-time PCR.
The conventional PCR and real-time PCR assays were compared. The PCR was able to detect the pathogen on naturally infected
gerbera plants and on symptomatic artificially infected plants collected 21 days after pathogen inoculation. The detection
limit was 5 × 103
P. cryptogea zoospores and 16 fg of DNA. Real-time PCR showed a detection limit 100 times lower (50 zoospores, 160 ag of DNA) and the
possibility of detecting the pathogen in symptomless artificially infected plants and in the re-circulating nutrient solution
of closed soilless cultivation systems. 相似文献
62.
Dario dOvidio Francesco Marino Emilio Noviello Enrico Lanaro Paolo Monticelli Chiara Adami 《Veterinary anaesthesia and analgesia》2018,45(2):183-189
Objective
To evaluate the efficacy and side effects of alfaxalone administered intramuscularly (IM) as a sedative agent in guinea pigs undergoing survey radiographs.Study design
Prospective clinical trial.Animals
A total of 30 client-owned guinea pigs.Methods
Following baseline assessments, 5 mg kg?1 alfaxalone was administered IM. Heart rate, arterial haemoglobin oxygen saturation, respiratory rate, rectal body temperature, palpebral reflex, response to toe and ear pinch, righting reflex, posture, jaw tone and reaction to manipulation were assessed before and after sedation at 5-minute intervals. The time elapsed from onset of sedation to return of locomotion and coordinated limb movements, the quality of recovery and the occurrence of undesired effects were observed and recorded.Results
The mean ± standard deviation onset of sedation was 2.7 ± 0.6 minutes. The physiological variables remained within normal ranges until completion of the procedure. Palpebral reflex and responsiveness to both ear and toe pinch were maintained during sedation. Neither hypoxaemia nor hypothermia was observed. The duration of sedation was 29.3 ± 3.2 minutes. Sedation and recovery were uneventful, and adverse effects were not observed.Conclusions and clinical relevance
In conclusion, 5 mg kg?1 of IM alfaxalone represents a valuable sedation protocol for healthy guinea pigs undergoing minor noninvasive procedures. Further trials are required to investigate its cardiovascular effects, clinical usefulness in unhealthy patients and its combined use with analgesics for procedures associated with nociception. 相似文献63.
Catherine A. Loughin DVM Diplomate ACVS ACCT Leonard J. Marino MD FAAP 《Veterinary surgery : VS》2014,43(7):888-894
64.
Brian P. Grossbard DVM Catherine A. Loughin DVM Diplomate ACVS ACCT Leonard J. Marino MD FAAP Joseph Sackman Scott E. Umbaugh BSE MSEE PhD Patrick S. Solt BSEE MSCS Jakia Afruz BSAPECE MSAPECE Peter Leando PhD DSc DAc Martin L. Lesser PhD EMT‐CC Meredith Akerman MS 《Veterinary surgery : VS》2014,43(7):869-876
65.
G. Marino A. Zanghi M. Quartuccio S. Cristarella M. Giuseppe G. Catone 《Journal of Equine Veterinary Science》2009,29(10):728-733
Nematodes of the genuses Strongylus and Setaria are able to erratically migrate to the equine testis and provoke characteristic inflammatory changes. Moreover, the presence of living parasites in scrotal testes is a scarce finding in which only the tracts and related lesions may be observed. In a 20-year experience of equine testes observed in abattoirs, 13 cases of atypical inflammatory lesions in scrotal testes were collected. On opening of the vaginal cavity, hydrocele was consistantly observed, and the Morgagni's appendix was enlarged, prominent, and reddish. Large, elongated, or irregular prominent red to yellow areas were visible on the testicular or epididymal serosa. Edematous enlargement of the epididymal head or tail was occasionally observed. In two cases, the described lesions were detected together with nematodes of the species Setaria equina, free and viable or encapsulated in the visceral and parietal layers of the tunica vaginalis, confirming the hypothesized cause. Histologically, the serosal lesions were interpreted as parasitic tracts, characterized by hemorrhage and leukocyte infiltrates in recent lesions or by granulation tissue with numerous macrophages in elderly ones. In the latter, areas of squamous metaplasia could be observed in the appendix testis and in the epididymal duct. Associated microscopic lesions included diffuse periorchitis, lymphocytic interstitial orchitis with mild to severe degeneration of seminiferous tubules, and vasculitis, epididymitis, and sperm granuloma. 相似文献
66.
67.
68.
M Xanthos 《Science (New York, N.Y.)》2012,337(6095):700-702
Polypropylene (PP) is a widely used plastic with consumer applications ranging from food packaging to automotive parts, including car battery casings. To differentiate it from other recyclable plastics, it is designated as #5. Here, the factors contributing to PP recycling rates are briefly reviewed. Considerations include collection and separation efficiency, processing chemistry, and market dynamics for the products derived from recyclates. 相似文献
69.
Marino MR Brown TJ Waddington DC Brockie RE Kelly PJ 《New Zealand veterinary journal》1992,40(1):24-27
Giardia intestinalis cysts were detected in faeces from 12.9% of 124 brushtail possums (Trichosurus vulpecula) trapped in Auckland, Hawke's Bay, the Wairarapa and Wellington; in faecal material from 61.0% of 77 ship rats (Rattus rattus) from areas of Auckland, Hawke's Bay and Wellington; and in faecal material from 25.3% of 182 house mice (Mus musculus) from the North Island. Possums and rodents in forested areas of Auckland and Wellington city water catchments were infected. The role of wild animals in maintaining and spreading Giardia in New Zealand is discussed. 相似文献
70.
Marc Kent Erin M. Beasley Karine P. Gendron Maëva C.M. Barozzi Christina Marino 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(6):592
A mixed-breed pygmy goat was presented for nonambulatory tetraparesis. Neurological examination was consistent with a C6 to T2 myelopathy. Initially, the goat was treated medically. Forty-three days later, magnetic resonance imaging (MRI) revealed an extradural mass compressing the cervical spinal cord. Magnetic resonance attributes of the mass were consistent with a slow-growing, fluid-poor lesion. The spinal cord was surgically decompressed. Epidural empyema secondary to Fusobacterium necrophorum was identified. Postoperative care consisted of anti-inflammatory medication, antimicrobials, and physical therapy. Ability to walk occurred by day 14 after surgery. Despite prolonged recumbency before surgery, the goat was clinically normal, and antimicrobials were discontinued on day 60 after surgery.Key clinical message:Epidural empyema can cause a compressive myelopathy which may result in varying degrees of paresis/paralysis. Clinical resolution and return of normal function occurred following the use of MRI to plan surgical decompression combined with extended use of antimicrobials. 相似文献