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101.
  总被引:2,自引:0,他引:2  
Investigation of a sight defect in a pedigree bull, born as a result of artificial insemination and ovum transplantation, led to the finding that the animal was persistently infected with bovine virus diarrhoea virus. Virus was cultured from blood and from nasal and ocular swabs and was present in semen in high titre. At necropsy, virus was cultured from a wide range of tissues. The pathological findings are described and discussed as are the potential hazards of such infections.  相似文献   
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Objective To perform a comprehensive phenotypic characterisation of 35 isolates of bacteria previously identified as haemolytic Pasteurella‐Actinobacillus and obtained from cattle and sheep. Design The 35 isolates that had been obtained from Australian animals, 30 from cattle and five from sheep, were compared with reference strains of the five recognised species of the genus MannheimiaM haemolytica, M glucosida, M granulomatis, M ruminalis and M varigena. Results Thirty‐four of the isolates could be confidently assigned to three species of the genus Mannheimia. Twenty‐nine were M haemolytica, with 25 being isolated from cattle and four from sheep. All but three of the bovine M haemolytica were isolated from pneumonic lungs. Of the three remaining bovine M haemolytica isolates, one was obtained in pure culture from a bovine milk sample and the other two as part of a mixed flora associated with a middle ear infection of a calf suffering mucosal disease. Of the four ovine M haemolytica isolates, two were isolated in pure culture from milk and two, also in pure culture, from pneumonic lungs. Three bovine isolates were identified as M granulomatis ‐ one from a tongue abscess, one from a jaw abscess and one from a lung showing suppurative bronchopneumonia. Two bovine isolates were identified as M varigena‐ one coming from an udder and the other from a spleen. The available diagnostic records provided no information on whether these isolates were associated with a disease process. The remaining isolate was obtained from an ovine tongue abscess and could not be assigned to a recognised species within the genus Mannheimia. Conclusion The study represents the first time that M haemolytica, M granulomatis and M varigena have been recognised as being present in cattle and sheep in Australia. Veterinary laboratories that encounter Pasteurella‐Actinobacillus‐like organisms from cattle and sheep should attempt as complete a characterisation as possible to help improve our knowledge of the disease potential of these organsims.  相似文献   
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Large-scale restoration of bottomland hardwood forests in the Lower Mississippi Alluvial Valley (USA) under federal incentive programs, begun in the 1990s, initially achieved mixed results. We report here on a comparison of four restoration techniques in terms of survival, accretion of vertical structure, and woody species diversity. The range of treatment intensity allowed us to compare native recolonization to direct seeding and planting of Quercus nuttallii Palmer, and to an intensive treatment of interplanting two species that differed in successional status (early successional Populus deltoides Bartram ex Marsh. ssp. deltoides, with the mid-successional Q. nuttallii). Native recolonization varied in effectiveness by block but overall provided few woody plants. All active restoration methods (planting and direct seeding) were successful in terms of stocking. Populus grew larger than Quercus, reaching canopy closure after 2 years and heights after 2 and 5 years of 6 and 12.7 m, respectively. Planted Quercus were significantly larger than direct seeded Quercus in all years, but only averaged 1.4 m in height after 5 years. Interplanting did not seem to facilitate development of the Quercus seedlings. The early success of the interplanting technique demonstrated that environmental benefits can be obtained quickly by more intensive efforts. Native recolonization can augment active interventions if limitations to dispersal distance are recognized. These results should provide landowners and managers with the confidence to use techniques of varying intensity to restore ecosystem functions.  相似文献   
104.
    

BACKGROUND

Arboviroses such as dengue, Zika and chikungunya represent a serious public health issue as a consequence of the absence of approved vaccines or specific antiviral drugs against the arboviruses that cause them. One way to prevent these diseases is by combating the vector mosquito, Aedes aegypti (Diptera), which has serine proteases in the midgut. Protease inhibitors are molecules that can block enzyme activity, impairing digestion and nutrition, which can lead to death. Thus, we purified and characterized a novel chymotrypsin‐trypsin inhibitor (LsCTI) from Lonchocarpus sericeus seeds and investigated its effect upon Ae. aegypti egg hatching, larval development and digestive proteases.

RESULTS

LsCTI showed a single protein band in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE), and the molecular mass determined by matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) was 8870.45 Da. Kinetics analyses revealed a noncompetitive type of inhibition and low inhibition constant (Ki) for chymotrypsin (8.24 x 10‐8 m ). The thermal resistance was remarkable, even at 100 °C for 180 min. The inhibitor concentration required for 50‐percent enzyme inhibition (IC50) of LsCTI was 4.7 x 10‐7 m for Ae. aegypti midgut larval enzymes. LsCTI did not affect egg hatchability at 0.3 mg mL‐1, but caused a high larval mortality rate (77%) and delayed development (37%).

CONCLUSIONS

LsCTI is a novel protease inhibitor with remarkable biochemical characteristics and is a potential tool to control Ae. aegypti development. © 2017 Society of Chemical Industry
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Book reviewed in this article: Guide to the Dissection of Domestic Ruminants . RE Habel Biology and Medicine of Rabbits and Rodents . JE Harkness and JF Wagner  相似文献   
108.
A simple model to compare and predict phosphorus (P) uptake behavior of plants may be useful to agronomists. A predictive equation based on Michaelis‐Menten kinetics was developed for this purpose. Kinetic parameters for use in the model were determined in an experiment using two cultivars of winter wheat grown for 21 days in 14 soil treatments, including seven P levels, each in fumigated and unfumigated soil. In another experiment, the same wheat cultivars were grown for 7, 14, or 21 days at one soil P level in both fumigated and unfiimigated soil. Using parameter values developed in the first experiment with multiple P levels and one time period, the model closely (R2=0.966, P<0.001) predicted P uptake in the second experiment with one P level over multiple time periods. The model could be a useful agronomic tool because of its simplicity and because no data need be collected from artificial growing conditions.  相似文献   
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