日粮锌、硒水平对肉鸡肠道黏膜屏障结构的影响

为了探讨微量元素锌和硒相互作用对肉鸡肠道黏膜屏障结构的影响，将24只1日龄AA肉鸡随机分3组，分别饲喂添加有高锌高硒(锌1000mg／kg、硒5mg／kg)、低锌低硒(锌34mg／kg、硒0．08mg／kg)或常锌常硒(锌50mg／kg、硒0．15mg／kg)的日粮45d后，观察肠黏膜上皮细胞、上皮内淋巴细胞和盲肠扁桃体的形态结构变化。结果表明：高锌高硒或低锌低硒组肉鸡的肠黏膜结构有明显的损伤，表现为肠黏膜上皮细胞萎缩，绒毛长度下降，上皮内淋巴细胞数量减少；盲肠扁桃体的弥散淋巴组织和淋巴小结中，淋巴细胞数量减少，细胞出现肿胀，有的核消失，结缔组织增生，淋巴小结萎缩。尤其是高锌高硒组的损伤最为严重。而常锌常硒组肉鸡肠黏膜和盲肠扁桃体的形态结构正常。结论：日粮中按锌50mg／kg、硒0．15mg／kg的比例添加，对于维持肠道黏膜的正常屏障结构是合适的。过高或过低的锌和硒对小肠黏膜有毒性作用，破坏其屏障功能；而且高锌和高硒可相互促进以增强其毒性作用。     

WTO协议的文体特征探析

应用现代文体学理论并结合语用学和认知语言学理论观点,尝试从字位与书写、词汇、句法层面描述和分析WTO英文协议的文体特征,希望对理解和翻译世界贸易法规和类似的经贸法规有所帮助。     

湖南省土地荒漠化和沙化监测布局与监测方法

我省土地荒漠化和沙化监测主要包括3个荒漠化监测站和5个沙化监测站，布设监测样地28个。确定了采集监测区域内气象、土壤、水文、生物等方面的信息和方法。     

论贫困山区县的生态旅游开发--以湖南新化县为例

生态旅游作为实现旅游可持续发展的重要途径正受到全球范围内的认同和追随。新化县是湖南中部的一个贫困山区县，对新化县的生态旅游资源作了全面的评价，从资源、区位、宏观环境等多方面对新化县生态旅游开发可行性进行了深入的分析，并提出了一系列生态旅游的开发策略。     

促性腺激素释放激素基因及其受体基因的研究进展

作者简要介绍了促性腺激素释放激素基因及其受体基因的位置、结构、表达、调控机理,并初步探讨了这两个基因与繁殖性能的关系。     

C肉毒杀鼠素在高寒草甸地区灭鼠技术中的应用

综述C肉毒杀鼠素草原灭鼠应用技术,充分肯定了C肉毒杀鼠素毒力强、适口性好、对非靶动物毒性低、作用缓慢、无二次中毒、易降解和适合规模灭鼠的特点,为鼠害防治提供了新的思路。     

The rates of maize growth and lignin biosynthesis change after root-applied chalcone

The effects of root-applied chalcone at 0.15 mmol L⁻¹ on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis.     

香菇烂筒病因与综合防治研究

本文通过对香菇烂筒重灾区进行生态环境、气象、植保等方面调查，应用微生物分离技术，对烂筒样品和土壤样品进行致烂菌的分离、筛选和回接试验，从中确定致烂菌菌株的分类地位和基本生物学特性，从而研制防治香菇烂筒的生态、物理和药物防治技术，提出综合治理措施。     

Cell proliferation and nestin expression in cortex and SEZ of MCAO rats

AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury.     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.