Isolation and Identification of E. coli in the Microbial-fermentation Bed of Piggery

To investigate the distribution and characteristic of E. coli in the microbial-fermentation bed of piggery, litters with different levels of fermentation were used for the isolation, identification and drug sensitivity test of E. coli, to analyze the pathogenicity and drug susceptibility of E. coli in the microbial-fermentation bed of piggery. The results showed that, E. coli could be isolated only from litters with low degree of fermentation (0<△E≤7.63), and totally 41 strains were isolated from litters with 10^-5 dilution, but none from litters with higher degree of fermentation (△E>7.63). The number of E. coli reduced along with the fermentation of litters. Among the 41 strains of E. coli, 15 were intestinal pathogenic strains, 9 of which had astA gene (22%), and 2 strains had sepA gene (4.8%). 11 strains were resistant to doxycycline, accounted for 27% of the total.The two strains containing sepA gene had strong resistance to doxycycline. In conclusion, the microbial-fermentation bed fermentation bed had a inhibitory effect on E. coli which promoted the application of fermentation bed and reusing of litters.     

松脂酸钠防治杨梅介壳虫的初步研究

通过室内毒力测定、安全性评价和田间药效等系列试验,评估30%松脂酸钠水乳剂(EW)、20%松脂酸钠可溶性粉剂(SP)和45%松脂酸钠可溶性粉剂(SP)用于防治杨梅介壳虫的可行性。结果表明:药剂处理4d后,3种松脂酸钠制剂(30%EW、20%SP和45%SP)对杨梅柏牡蛎蚧的致死中浓度(LC50)为222.856~278.199mg·L-1,98%噻嗪酮原药的LC50为73.967mg·L-1,可见松脂酸钠3种制剂具有一定的杀虫活性,但其室内毒力显著低于化学药剂噻嗪酮。室内安全性评价结果表明,松脂酸钠3种制剂在田间使用最高剂量的1、2、4倍情况下,对东魁杨梅、荸荠种、丁岙梅3种杨梅均具有较好的安全性。浙江、江西、福建三地田间药效试验结果表明,松脂酸钠3种制剂对杨梅柏牡蛎蚧、矢尖盾蚧、榆牡蛎蚧均具有较好的防治效果。30%EW、20%SP和45%SP分别稀释400、300和200倍时,防治效果均在75%以上(75.67%~93.13%),达到与化学药剂噻嗪酮或噻虫嗪相当的防治效果。     

施用污泥对小麦生物性状及其产量的影响

在河西绿洲生态条件下,研究了污泥的施入方式及施用量对于小麦生物性状及其产量的影响。结果表明,污泥施用量为9 000 kg/hm2、复种绿肥还田处理下的小麦籽粒产量和生物产量表现最好。相对于常规纯施化肥处理,施入污泥处理可以改善小麦的生物性状。     

混合痔5种疗法临床比较研究

目的探讨混合痔几种治疗方法的疗效.方法对603例患有混合痔的患者采用高频电容场痔核疗法（HCPT）、内扎外切疗法、单扎微波疗法、痔根断疗法、枯切疗法进行治疗.结果经对比分析发现：HCPT疗法治疗痔疮具有痛苦小、并发症少、疗程短、费用低、治愈率高、复发率低等优点.结论 HCPT疗法具有诸多优点,患者易于接受.     

丰花月季愈伤组织的诱导及细胞悬浮培养

分别以丰花月季叶片、叶柄和茎段为外植体诱导出愈伤组织,并建立细胞悬浮系。结果表明,丰花月季叶片和茎段是诱导愈伤组织和进行细胞悬浮培养的理想外植体;诱导松散型愈伤组织的最佳激素浓度配比是NAA 4 mg·L-1+2,4-D 1 mg.L-1+BA 1 mg.L-1;细胞悬浮培养细胞最佳起始密度为1.5×104～3.0×104个.mL-1时;继代培养4～5代时,可得到稳定的悬浮细胞系。     

人源菌群相关仔猪模型的建立——仔猪的饲养方法

主要介绍了人源菌群相关仔猪模型建立的中心环节,即仔猪的饲养方法。结果表明,HFA仔猪模型的建立方法已基本成熟。     

Observation on the modifying activity of the protein from Elytrzgia repens rhizome for ice crystal

In winter,spring and summer,the rhizome of wild Elytrzgia repens of Heilongjiang Province was selected to extract the soluble which whole protein and the apoplastic protein,and analyzed by SDS-PAGE.The result indicated that there were two specific polypeptides in two types protein from winter;their relative molecular weight were identified as 52 ku and 26 ku by analyzing software:the apoplastic protein from winter had the ability of modifing the growth of ice crystal which appeared hexagonal in shape observed with the phase-contrast photomicroscope.So the apoplastic protein from winter has the antifreeze characters and the 52 ku protein is more likely the antifreeze protein.     

上海城市公园典型植物群落美景度评价

城市公园植物群落作为城市公园的重要组成部分,其景观美学质量具有重要的研究价值。本文依据上海城市公园的类型、区位和建成时间选择典型公园作为研究样本,以公园中具代表性的32个植物群落作为研究对象,运用美景度评判法对其景观美学评价。结果表明：按照SBE平均值排列不同群落类型,从高到低依次是：落叶阔叶林（33.399 5）、常绿落叶阔叶混交林（26.837 2）、常绿阔叶林（19.235 1）、针阔混交林（10.169 3）、常绿针叶林（1.028 4）、落叶针叶林（-0.066 9）和竹林类（-9.135 9）,然后从上述群落配置模式、外貌和生长状况等方面进行分析,得出影响植物群落美景度的因子并提出了提高群落美景度的方法和途径。     

植物源病毒抑制剂WCT-II控制烟草花叶病毒(TMV)的作用机理初探

用植物源病毒抑制物WCT- 直接处理烟草花叶病毒(TMV)病毒粒体,经枯斑半叶法接种和电镜检测发现,处理后的病毒致病力明显下降,病毒粒体大量发生断裂、略变弯曲等畸变现象,WCT- 对TMV的枯斑抑制率为86.6%;WCT- 处理烟草后,其叶片匀浆和胞间液的蛋白经SDS-PAGE不连续电泳发现,处理后的烟草叶片胞间在第5天至第9天和第7天分别存在1条酸溶性蛋白和1条碱溶性蛋白。上述研究结果表明,WCT- 不仅对TMV病毒粒体具有体外钝化作用,而且能诱导植物产生病程相关蛋白,提高烟草抵抗TMV侵染的能力。