茶卡盐湖中耐盐抗真菌芽孢杆菌的分离和鉴定

本试验从茶卡盐湖水及淤泥中分离得到了51株细菌,通过16S rDNA及gyrB基因测序分析初步确定了菌株的分类地位,并测定了各菌株对苹果根腐病菌Fusarium spp.、辣椒疫霉病菌Phytophthora capsici等13株病原菌的抑菌谱及对NaCl的耐受性。共筛选获得13株对13种真菌病害均有抑菌作用的菌株,13株广谱生防菌株在NaCl浓度为10%的LB培养基上都能正常生长,其中菌株10和27在NaCl浓度为15%的LB培养基中的生长速度显著高于生防菌B916。盐湖环境为耐盐生防菌的筛选提供了丰富的资源,本研究获得的耐盐广谱生防菌对盐碱地真菌病害的防治提供了菌株资源。     

干旱胁迫对杂种马褂木形态特征的影响

杂种马褂木(Liriodendron chinense×L. tulipifera)是一种生长快、材质好、树形美、观赏价值高的优良树种,现已广泛应用于园林绿化和用材林培育。为了了解杂种马褂木的抗旱性,笔者对南京市8年生的杂种马褂木在夏季干旱条件下的生长情况进行了调查研究。结果表明,杂种马褂木具有较强的抗旱性,但是在严重的干旱胁迫下,杂种马褂木叶片含水率和叶绿素含量明显降低,叶片发生黄化和脱落。在下蜀黄土上,如果土壤含水率低于12%,杂种马褂木会出现明显的叶片黄化和脱落现象,故在土壤含水率降低到12%之前有必要补充水分。     

Seismic performance of first floor corner joints of high-rise reinforced concrete frame structure

Crushing risk of corner column will increase substantially due to first floor corner joint shear failure of high-rise reinforced concrete frame structure under bi-directional loading and high axial compressive load caused by earthquake action. Therefore,     

Effects of kefir on coccidial oocysts excretion and performance of dairy goat kids following weaning

The aim of this study was to investigate effects of kefir, a traditional source of probiotic, on coccidial oocysts excretion and on the performance of dairy goat kids following weaning. Twin kids were randomly allocated to one of two groups at weaning. Kids of the first group received 20 ml of kefir daily for 6 weeks (KEF), while kids in the control group were given a placebo (CON). Individual faecal samples were regularly (n = 18 per kid) taken to quantify the number of coccidial oocysts per gram of faeces (OpG). There were no differences between the groups in terms of body weight development (P > 0.05) and feed consumption. Kids of both groups were not able to consume enough feed to meet their nutrient requirements during the first 3 weeks following weaning. KEF had a lower frequency of OpG positive samples than CON (P = 0.043). Kefir did not affect the maximum oocyst excretion and age of the kids at the highest oocyst excretion (P > 0.05). KEF shed numerically 35% lower coccidial oocysts than the controls, which corresponded to a statistical tendency (P = 0.074) in lowering Log-OpG in comparison to CON. While KEF had a lower frequency of OpG positive samples and tended to shed lower OPG by around one-third, the frequency of diarrhea, level of highest oocyst excretion, and performance of the kids remained unaffected. Therefore, it is concluded that overall effects of kefir do not have a significant impact on sub-clinical infection and performance in weaned kids under relatively high-hygienic farming conditions.     

酸化猪炭对土壤中不同形态磷质量分数及相互转化的影响

通过培养试验,采用修正的Hedley磷形态分级方法,以不添加任何肥料处理为空白对照,以施加未经处理的猪炭、过磷酸钙和钙镁磷肥处理为对比,研究酸化猪炭对土壤中各形态磷质量分数及其相互转化的影响。结果表明:与对照相比,施用猪炭和酸化猪炭可使土壤电导率分别提高24%和14%,而对土壤pH值无显著影响。在施加量相同的情况下,酸化猪炭提高土壤活性磷和中稳活性磷占全磷百分比的效果优于猪炭处理;且与施磷量相同的过磷酸钙和钙镁磷肥处理无显著差异。与空白对照相比,施用酸化猪炭、猪炭和2种磷肥均可以显著（P < 0.05）提高活性磷的质量分数,且各处理之间对于活性磷质量分数的提高无显著差异;中稳活性磷占总磷的百分比提高36.2%~77.2%,其提高程度为:酸化猪炭>过磷酸钙>钙镁磷肥>猪炭;非活性磷占总磷的百分比显著（P < 0.05）下降,与活性磷和中稳活性磷所占比例均呈极显著（P < 0.01）负相关。施用酸化猪炭可以提高土壤中各形态磷的质量分数,同时把土壤中非活性磷的比例维持在较低水平。施用酸化猪炭对土壤中各形态磷质量分数及转化影响的效果与2种化学磷肥无显著差异。在农业生产中,经酸化处理的猪炭来替代过磷酸钙和钙镁磷肥具有潜在的可行性。     

红花木AFLP指纹图谱的构建

为了获得红花檵木的清晰AFLP指纹图谱,以进一步研究红花橙木种质资源的鉴别与分类问题, 、以红花檵木的嫩叶、成熟叶、果实为材料,采用改进后的CTAB+Tris-HCI洗涤法、CTAB法、SDS+Tris-HCI洗涤法、SDS法,获得了HMW基因组DNA,其中以CTAB+Tris-HCI洗涤法提取的成熟叶片的DNA的纯度和浓度都很高,且获得了清晰的AFLP指纹图谱,这为AFLP技术在红花橙木种质资源的研究与应用奠定了基础.     

Coordinated pressure control of a regenerative braking system based on a hybrid electric vehicle with an integrated startor and generator

With a focus on a Changan hybrid electric vehicle (HEV) with an integrated startor and generator (ISG), strategies for braking force distribution and coordinated pressure control are put forward, and a dynamic braking control model is constructed for off     

Isolation of Sesquiterpenoids and Steroids from the Soft Coral Sinularia brassica and Determination of Their Absolute Configuration

Two undescribed rearranged cadinane-type sesquiterpenoids (1–2), named sinulaketol A-B, together with one new chlorinated steroid (3), one new gorgosterol (4), one known sesquiterpene (5), one known dibromoditerpene (6) and two known polyhydroxylated steroids (7–8) were isolated from the soft coral Sinularia brassica. The structures of these compounds were established by extensive spectroscopic analysis, including HRESIMS, 1D, and 2D NMR spectroscopy. Their absolute configurations were also determined by the ECD calculations and DP4+ probability analysis. Antileishmanial activity of compounds 1–8 was evaluated in vitro against the amastigote forms of Leishmania donovani, in which compounds 3, 6, and 7 inhibited the growth of L. donovani by 58.7, 74.3, 54.7%, respectively, at a concentration of 50 μM. Antimicrobial effect of the isolated compounds were also evaluated against Candida albicans, Staphylococcus aureus, and Escherichia coli. Compound 6, a brominated diterpene, exhibited antimicrobial effect against S. aureus.     

野外人工模拟降水条件下荒草坡产流产沙试验

采用人工模拟降水的方法,以5种不同的降水强度研究浙江省临安市青山湖流域内荒坡的产流产沙特征。结果表明,降水强度越大,产流时间越短,其径流量、产沙率和产沙量都越大,降水强度为1．66mm·min^-1时。产流时间仅为47s,降水约30min后,小区径流总量可达到0．898m^3,次侵蚀模数达到了30t·km^-2。降水强度与产流时间呈较好的负相关对数关系,与径流系数以及次侵蚀模数呈指数相关,与径流总量呈幂函数相关。相关系数分别达到了一0．9655．0．9218,0．9635和0．8736,均达到极显著水平（P〈0．01）。图3表1参11