Resveratrol inhibits chondrosarcoma via mitochondrial and PI3K/Akt signaling pathways

AIM：To investigate the inhibitory effects of resveratrol on chondrosarcoma and the relation with mitochondrial and PI3K/Akt pathways. METHODS：Chondrosarcoma SW1353 cells were treated with resveratrol at concentrations of 25, 50 and 100 μmol/L for the time intervals of 24 h, 48 h and 72 h. The viability and apoptosis of the SW1353 cells in the presence or absence of resveratrol were analyzed by CCK8 assay and Hoechst 33258 staining, respectively. The protein levels of Bcl-2, Bax, activated caspase-3, Akt and p-Akt were detected by Western blotting. The cell migration ability was determined by wound scratch assay. RESULTS：Exposure of the cells to resveratrol resulted in a decrease in the cell viability in a dose- and time-dependent manner (P<0.05). visible nuclei with apoptotic characteristics in resveratrol group were observed. The protein levels of activated caspase-3 and Bax were increased, and Bcl-2 and p-Akt were decreased compared with control group. The total Akt were not significantly changed. Resveratrol also significantly reduced the migration of tumor cells. CONCLUSION：Resveratrol induces apoptosis of chondrosarcoma, which plays a role of part through mitochondrial and PI3K/Akt signaling pathways.     

Effect of small interfering RNA on expression of β2M in pre-differentiated bone marrow mesenchymal stem cells

AIM：To study the effect of small interfering RNA (siRNA) on the expression of beta 2-microglo-bulin (β2M) in pre-differentiated bone marrow mesenchymal stem cells (BMSCs). METHODS：The β2M siRNA was transfected into the pre-differentiated BMSCs with Lipofectamine 2000. BMSCs were divided into transfection group, blank control group and negative control group. The expression of β2M at mRNA and protein levels was determined by real-time qPCR, Western blotting and laser confocal microscopy. The productions of aggrecan and type II collagen in pre-differentiated BMSCs were determined by toluidine blue staining and type Ⅱ collagen immunofluorescence. RESULTS：The results of real-time qPCR, Western blotting and laser confocal microscopy showed that siRNA successfully inhibited the expression of β2M at mRNA and protein levels in the pre-differentiated BMSCs. The results of toluidine blue and type Ⅱ collagen immunofluorescence staining showed that siRNA does not affect the productions of aggrecan and type Ⅱ collagen in the pre-differentiated BMSCs. CONCLUSION：siRNA targeting β2M reduces the expression of β2M in the pre-differentiated BMSCs and does not affect the chondrocyte characteristics of pre-differentiated BMSCs.     

汕头市农产品质量安全监督与风险评估探析

为了提升农产品品质,加强对有害因子的分析与识别,提升社会公共安全问题,分析了汕头市农产品质量监督的现状与存在的问题,研究和探讨了如何加强农产品质量安全监督与风险评估建设,达到提升农产品质量监督与监测能力和推进农产品从源头、流通领域到消费者的食用安全的目的,全方位地确保农产品质量安全,确保民众食用健康和社会稳定。     

大棚不同地膜栽培对蔬菜生长及产量的影响

通过在大棚内进行不同地膜,研究了大棚内麻地膜覆盖对辣椒、黄瓜生长及产量的影响。试验结果表明,大棚内麻地膜覆盖栽培促进蔬菜生长,提高辣椒、黄瓜产量。麻地膜覆盖栽培辣椒、黄瓜比聚乙烯农用黑膜增产5．2％、14．5％,比CK（不覆盖）增产24．7％、23．0％,与对照相比均达到显著差异。     

与黄瓜霜霉病抗性相关基因连锁的分子标记研究

以黄瓜抗霜霉病和感霜霉病亲本组合(Q9×Q10)的F2分离群体为试材,采用BSA法筛选到了1个AFLP引物组合P11M70在抗病池和感病池间表现多态,抗病池和抗病亲本均具有大小约304 bp的特异谱带,感病池和感病亲本均扩增出了大小约为301 bp的特异片段。经F2单株验证及连锁分析,该特异片段与霜霉病抗病相关基因相连锁,连锁距离为5.22 cM。对特异片段的测序结果显示,两片段的大小分别为304 bp和301 bp,且2个片段的差异仅为3个"A"碱基的插入或缺失以及1个"C-T"的碱基突变。将该AFLP标记成功地转换为了简单实用的SCAR标记。     

袋栽金针菇2潮后脱袋增产技术研究

为满足商品性要求,袋栽金针菇2潮后一般不再进行出菇管理。实际上,这时的菌棒仍有出菇趋势,由于塑料皮限制其生长,且阻碍补水补养,致使无法产生商品性好的菇,培养基中养分无法利用充分。试验中割破塑料皮,采用脱袋排场盖膜法和脱袋拱棚盖膜法进行继续出菇管理,并设置对照。结果表明,2种措施可分别再出菇3潮,生物学效率分别提高34％和28％,商品性满足三级以上标准,是可推广的金针菇增产措施。     

浙北黑木耳大棚栽培与露地栽培对比分析

大棚栽培桑木耳试验成果显示,在大棚中排场,可有效提高产量和效益,12月5日大棚内排场产量极显著高于11月5日露地排场和12月5日露地排场的,拉长了桑木耳制棒时间,有利于规模化、合作社化生产。     

饲料糖种类和水平对青鱼、鲫生长和体成分的影响

用分别含20%（低）、40%（高）葡萄糖和糊精的等氮（粗蛋白为35%干物质）,等能（16.4 kJ/g）饲料分别饲养青鱼（Mylopharyngodn piceus）和鲫鱼（Carassius auratus）8周,研究饲料糖种类和水平对青鱼和鲫的生长、体成分的影响.青鱼和鲫鱼平均体质量分别为2.14 g和6.75 g.结果表明,两种鱼都是糊精组增重率、饲料效率、蛋白质效率显著高于葡萄糖组,提示两种鱼对糊精利用效率比葡萄糖高.饲料糖含量低时增重率、饲料效率、蛋白质效率相对高,提示两种鱼对脂肪作为能源的利用效率高于糖.两种鱼糊精组脂肪蓄积率显著高于葡萄糖组,体脂、肝脂、肌脂含量也高于葡萄糖组,提示糊精比葡萄糖更容易以脂肪形式积累在体内.青鱼对实验饲料的利用效率高于鲫鱼,推测这与能量代谢有关.[中国水产科学,2006,13（3）：452-459]     

渔船节能开发和推广要点

1 渔船节能主要措施 过去的20年，我国在渔船节能方面采取的主要措施有： （1）改进船型以减少推进阻力； （2）采用低转速大直径螺旋桨和渔船尾部水动力附加节能装置；     

甘露青鱼肌肉营养分析和评价

取材太湖流域无锡甘露水产公司养殖的大青鱼,采用化学及仪器分析手段,对青鱼含肉率及肌肉营养成分进行了分析测定,含肉率为71 08%,肌肉中蛋白质含量为16 56%,脂肪含量为14 08%,其中70 73%为不饱和脂肪酸,18种氨基酸总量为14 91%,8种人体必需氨基酸的含量为6 07%,4种鲜味氨基酸的含量为5 62%,还含有多种微量元素,通过大量基础数据综合评价甘露青鱼的营养价值。