雄蚕杂交种的应用研究

总结了4年8个蚕期利用家蚕性连锁平衡致死第转育改良组配成的7对雄蚕杂交组合的饲养试验。结果表明雄蚕杂交种具有食桑省、叶丝转化率高、能够大幅度提高蚕出丝率、茧丝质量优异等优点，具有广阔的推广应用前景，对于改造提升传统蚕丝业具有重要意义。     

蜂胶高生物活性黄酮组分的研究

本文探讨了蜂胶总黄酮中最具活性的黄酮类物质的提取分离、官能结构特征与功能关系、可能的功效,进而提出了如何看待蜂胶总黄酮含量高低的问题.     

高等级公路植被护坡工程技术发展初探

依据恢复生态学原理,在探讨植被护坡技术现状的基础上,对高等级公路植被护坡工程技术中的生物治理和工程治理进行分析,提出了植被护坡工程设计原则、技术发展趋势和建议.     

组织培养条件下苹果Ty1-copia类逆转座子的转录活性

研究了苹果基因组中Tyl—copia类逆转座子的转录活性、多样性及其甲基化水平。在培养基MS＋2，4-D2mg／L＋BA0．2mg／L＋NAA0．5mg／L上诱导了1个月的愈伤组织中用RT—PCR方法检测到了270bp的目的条带，而对照、继代组培苗和不含2，4-D的愈伤中均没有目的条带的扩增，说明一定浓度的2，4-D可以诱导苹果中Tyl—copia类逆转座子的表达活性。将目的条带回收、克隆和测序后进行分析：所克隆的21条序列虽都为Tyl-copia类逆转座子逆转录酶保守序列，但存在很大的异质性，21个克隆的核甘酸序列变化范围为196—290bp；翻译成氨基酸后存在缺失、插入、移框和终止密码子突变，7个克隆在氨基酸保守序列‘SLYGLKQ’处有1—2氨基酸位点的突变，说明这些序列对应的逆转录酶多数可能已失去了其原有功能。将21条序列进行聚类分析，除ART20外，其它克隆可聚为3类，并分别与GenBank上某些物种的相应序列同源性很高，表明在不同物种间可能存在逆转座子的横向传递作用。甲基化试验表明苹果基因组内Tyl-copia类逆转座子甲基化水平高。这些都表明Tyl-copia类逆转座子对苹果基因型的多样性及基因组的进化具有重要意义。     

彩色甜椒花药培养若干影响因子的研究

利用硝酸银、ATP钠盐、NAA、KT进行了四因素三水平正交试验,材料为彩色甜椒品种7160的花药。结果表明,硝酸银是影响彩色甜椒花药培养胚状体诱导率的最主要因素,在其浓度为1.0mg/L时胚状体诱导率最高;ATP钠盐为次主要因素,在其浓度为100.0mg/L时效果最好。KT比NAA对胚状体诱导率的影响大;KT浓度1.0或1.5mg/L时较好。     

2004年冬成都市外来园林植物冻害调查及建议

作者2005年春调查了成都市外来园林植物冻害情况,发现：（1）外来园林植物受冻害影响较为普遍,41种植物在不同程度上受害;（2）冻害影响存在区域差异,郊区冻害比城区严重.分析了冻害原因,并对园林植物引种提出建议.     

论西部大开发中张掖市生态环境建设问题

张掖市长期受干旱、大风及沙尘暴影响,生态环境极为恶劣,制约着西部大开发建设进程。其存在的重要问题是:水资源匮乏,植被破坏严重,覆盖率低等;针对具体情况提出:改善水资源条件和恢复植被是张掖地区生态环境建设的重点。     

Relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats

AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp／dt_max) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp／dt_max) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp／dt_max and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp／dt_max and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction.     

Cell proliferation and nestin expression in cortex and SEZ of MCAO rats

AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury.     

Traumatic shock and systemic inflammation

Trauma is the No.1 cause of death in persons under the age of 45 and the No.3 cause of all deaths. Many trauma patients die of multiple organ failure (MOF), induced by systemic inflammatory response syndrome (SIRS). This paper reviews the relations of traumatic shock with systemic inflammation.