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111.
T. D. G. Watson † D. Gaffhey C. T. Mooney† H. Thompson‡ C. J. Packard J. Shepherd 《The Journal of small animal practice》1992,33(5):207-212
Hyperchylomicronaemia was identified in a four-week-old Siamese kitten with lethargy, in-appetence, hindlimb ataxia and profound anaemia. The kitten was euthanased and at necropsy a thrombus was found occluding the caudal aorta. Two littermates later presented with lethargy, inappetence and hypertriglyceri-daemia which resolved after being weaned on to a low fat diet. A similar condition was subsequently diagnosed in a kitten born to the same sire but a different queen. The expression of hyperchylomicronaemia in two related litters was suggestive of an inherited, familial defect in the function of lipoprotein lipase (LPL). The activity of this enzyme was reduced in all three parents, the two recovered cases and two related, but apparently unaffected kittens, compared with a control group of unrelated cats belonging to the breeder. This reduction in activity was not attributable to defective activation of LPL by its serum cofactor apolipoprotein C-II or the presence in plasma of a factor that inhibited LPL. The gene that codes for LPL was examined by restriction fragment length polymorphism analysis using a human LPL cDNA probe. The results showed that the cat has a similar, but not identical, LPL gene to man. However, there were no differences in the restriction fragment patterns obtained from affected, unaffected and control animals. 相似文献
112.
113.
齐卡商品肉兔在不同饲养条件下的育肥效果 总被引:1,自引:0,他引:1
作者在不同条件下,采用全窝育肥法,重复两年、两个季节测定了齐卡商品肉兔的育肥效果;探讨了温度、季节、营养水平、育肥始重对育肥效果的影响。结果表明:在试验场育肥250只齐卡商品兔,90日龄育肥重2.33~2.53公斤,日均增重31克以上,耗料指数3.31:1,育肥成活率95%以上,屠宰率52%,净肉率80%,肉骨比5.35:1,兔肉品质正常。在农村条件下育肥180只,90~100日龄育肥重2.2~2.4公斤,耗精料指数2.55:1,育肥成活率88%~93%。饲养齐卡商品兔与饲养二元杂交兔相比,每只可增加经济收入20%以上。育肥季节和始重明显影响育肥未重(P<0.05)。依据本试验,作者建议齐卡商品兔适宜35日龄断奶,育肥始重应不低于600克;寒冷季节育肥,育肥第一月的室温应不低于10℃。育肥适宜营养水平建议消化能(DE)2600~2700大卡/公斤,粗蛋白(CP)18%,粗纤维(CF)12%以上。 相似文献
114.
F C Battaglia 《Journal of animal science》1992,70(10):3258-3263
Fetal amino acid nutrition and metabolism have been studied primarily in pregnant sheep. The umbilical uptake of amino acids changes during gestation, but at both mid- and late gestation the total supply exceeds that required for growth. Weight-specific protein synthetic rate decreases with increasing gestational age, and these changes are proportional to the changes in metabolic rate. The use of multiple tracer methodology coupled with measurement of net tracer fluxes into and out of fetal and placental tissues can be used to delineate amino acid metabolism in considerable detail. Such studies demonstrate that even essential amino acids can be oxidized extensively by the fetus. The oxidation rate of leucine exceeds its rate of accretion in tissue proteins. Glycine metabolism is unique in several ways; there is a large umbilical uptake of glycine without a measurable uterine uptake. In late gestation there is no significant umbilical uptake of serine, although there is a significant uterine uptake, suggesting net uteroplacental utilization. Glycine is oxidized within the fetal liver and used for serum production. The interorgan exchange of amino acids between the fetal liver and placenta is clearly of major importance for serine and glycine metabolism and is likely to be of major importance for most nonessential amino acids. 相似文献
115.
E Bezirtzoglou M B Romond C Romond 《Comparative immunology, microbiology and infectious diseases》1992,15(1):71-74
Studies were undertaken to determine the regulation of the bacterial intestinal implantation in 19 newborns delivered by caesarean section. Correlation was made with the infant feeding mode. The effect of human milk seemed to be the result of B. bifidum proliferation, in contrast to artificial alimentation that seemed to favour C. perfringens implantation. The question was raised by us as to whether this opposition was only related to alimentation. In fact, B. bifidum itself also had an effect as demonstrated by the lower mean counts of C. perfringens in bottle-fed infants carrying the bifido-bacterial flora (P = 0.05). None of the other faecal bacteria investigated in this study led to the same decrease. 相似文献
116.
将构建的pBST2~6工程菌质粒用限制性内切酶BamHI/BglⅡ酶切,经琼脂糖凝胶电泳和电洗脱,回收147bp的目的ST1基因。随之将该基因分别重组到能有效表达K99菌毛抗原和LacZ酶的pGK99之K99基因BglⅡ位点和pUC18的BamHI位点中。通过ST1基因探针菌落原位杂交、特定酶切分析及DNA序列分析,筛选并鉴定出了理想重组子,从而构建出了能分别表达ST1融合基因产物的工程菌株pSK219和pXST1。 相似文献
117.
Soil pH declined from 5.9 to 5.0 in 8 years beneath plantations of Eucalyptus saligna (Sm.) in Hawaii. In stands of Albizia falcataria, (L.) Fosberg, the soil pH change was more dramatic, declining from 5.9 to 4.6. We measured several components of soil acidity beneath four mixtures of the two tree species to gain insight on the processes responsible for the decline in soil pH. These components were studied using an empirical method of comparing acid quantity, degree of neutralization (depletion of base cations), and acid strength. The decline in soil pH differed between species as a result of differences in the degree of neutralization of the soil exchange complex; the larger decrease in soil pH under Albizia was produced by greater acidification of the exchange complex. Empirical titration curves suggested that differences in acid strength moderated the divergence in soil pH beneath the species. Had the acids accumulating in the soil under Albizia been as strong as those in the Eucalyptus soil, the difference in soil pH would have been greater. Though the two species had contrasting effects on soil pH, the differences in degree of neutralization, responsible for the pH decline, were small compared with differences in the amount of cations stored in tree biomass. Continued supply of nutrient cations (from weathering or fertilization) will ultimately control both the extent of soil pH decline and the level of productivity sustained by the forest. 相似文献
118.
A technique is described for biopsy of the bovine udder, employing sedation and local anaesthesia. Tissue samples of approximately 5 g were obtained by electrocautery from two quarters of the udder of a cow laterally recumbent. Care was taken to ensure complete haemostasis which was achieved by electrocoagulation and ligation. Postoperative recovery was rapid, and loss of yield was no greater in biopsied glands than in control glands of the same cow. Yield from all quarters returned to preoperative levels within 48 h. 相似文献
119.
地膜覆盖对棉枯萎病发生的影响 总被引:1,自引:0,他引:1
地膜覆盖显著降低了棉花枯萎病的发病率与病情指数,极显著地提高了棉花的产量。 相似文献
120.