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991.
992.
Pharmacokinetic indices for cefovecin after single‐dose administration to adult sea otters (Enhydra lutris) 下载免费PDF全文
E. A. Lee B. A. Byrne M. A. Young M. Murray M. A. Miller L. A. Tell 《Journal of veterinary pharmacology and therapeutics》2016,39(6):625-628
Seven sea otters received a single subcutaneous dose of cefovecin at 8 mg/kg body weight. Plasma samples were collected at predetermined time points and assayed for total cefovecin concentrations using ultra‐performance liquid chromatography and tandem mass spectrometry. The mean (±SD) noncompartmental pharmacokinetic indices were as follows: CMax (obs) 70.6 ± 14.6 μg/mL, TMax (obs) 2.9 ± 1.5 h, elimination rate constant (kel) 0.017 ± 0.002/h, elimination half‐life (t1/2kel) 41.6 ± 4.7 h, area under the plasma concentration‐vs.‐time curve to last sample (AUClast) 3438.7 ± 437.7 h·μg/mL and AUC extrapolated to infinity (AUC0→∞) 3447.8 ± 439.0 h·μg/mL. The minimum inhibitory concentrations (MIC) for select isolates were determined and used to suggest possible dosing intervals of 10 days, 5 days, and 2.5 days for gram‐positive, gram‐negative, and Vibrio parahaemolyticus bacterial species, respectively. This study found a single subcutaneous dose of cefovecin sodium in sea otters to be clinically safe and a viable option for long‐acting antimicrobial therapy. 相似文献
993.
ABSTRACT Phytophthora cinnamomi isolates collected from 1977 to 1986 and 1991 to 1993 in two regions in South Africa were analyzed using isozymes. A total of 135 isolates was analyzed for 14 enzymes representing 20 putative loci, of which four were polymorphic. This led to the identification of nine different multilocus isozyme genotypes. Both mating types of P. cinnamomi occurred commonly in the Cape region, whereas, predominantly, the A2 mating type occurred in the Mpumalanga region of South Africa. A2 mating type isolates could be resolved into seven multilocus isozyme genotypes, compared with only two multilocus isozyme genotypes for the A1 mating type isolates. Low levels of gene (0.115) and genotypic (2.4%) diversity and a low number of alleles per locus (1.43) were observed for the South African P. cinnamomi population. The genetic distance between the Cape and Mpumalanga P. cinnamomi populations was relatively low (D(m) = 0.165), and no specific pattern in regional distribution of multilocus isozyme genotypes could be observed. The genetic distance between the "old" (isolated between 1977 and 1986) and "new" (isolated between 1991 and 1993) P. cinnamomi populations from the Cape was low (D(m) = 0.164), indicating a stable population over time. Three of the nine multilocus isozyme genotypes were specific to the "old" population, and only one multilocus isozyme genotype was specific to the "new" population. Significant differences in allele frequencies, a high genetic distance (D(m) = 0.581) between the Cape A1 and A2 mating type isolates, significant deviations from Hardy-Weinberg equilibrium, a low overall level of heterozygosity, and a high fixation index (0.71) all indicate that sexual reproduction occurs rarely, if at all, in the South African P. cinnamomi population. 相似文献
994.
<正>通过植物介导的RNAi能够沉默棉铃虫蜕皮调节转录因子Ha HR3基因。棉铃虫取食含有dsRNAs的转基因烟草后,其Ha HR3基因的转录水平及蛋白表达水平均被有效抑制,导致棉铃虫幼虫生长发育畸形或死亡(尹富仕等,2010;Xiong et al.,2013)。本研究利用透射电镜观察棉铃虫取食转Ha HR3基 相似文献
995.
Shanice R. MajorClifford L. Fontenot Jr. John A. Pojman Sr.John A. Pojman Jr. Mark E. Merchant 《Comparative immunology, microbiology and infectious diseases》2011,34(2):115-121
Some animals routinely endure serious injuries from predators or during intraspecific territorial conflicts. Such is the case for Amphiuma tridactylum, an aquatic salamander that lives in an environment rich in potentially infectious microbes, apparently with rare or no pathogenic infection. Some vertebrates possess innate immune mechanisms, but whether this is the case for Amphiuma is unknown. To assess this potential, plasma from 19 A. tridactylum was pooled and used for characterisation of serum complement activity. The ability of A. tridactylum plasma to hemolyse unsensitised sheep red blood cells (SRBCs) was titer-dependent, with low activity observed even at high plasma titers. The kinetic characterisation of SRBC hemolysis revealed that significant activity could be measured within 10 min of incubation, and maximal activity occurred within 60 min. The SRBC hemolysis by A. tridactylum plasma was also temperature-dependent, with maximal activity at 30 °C. In addition, this activity was sensitive to mild heat treatment, with 96% of activity inhibited by incubation at 56 °C for 30 min. The SRBC hemolysis could also be inactivated by pretreatment of the plasma with proteases, indicating that this activity was protein dependent. The activity required divalent metals ions, with activity inhibited by EDTA, citrate, or phosphate. However, the chelator-inhibited activity could be restored by the addition of excess Ca2+ or Mg2+, but not Cu2+ or Ba2+, indicating specificity of the divalent metal ion requirement. The sensitivity to heat, proteases, and divalent metal ion chelators strongly suggests that A. tridactylum plasma-mediated hemolysis of SRBCs is mediated by the serum complement system of proteins. 相似文献
996.
Resynchronization of estrus in beef cattle: ovarian function, estrus and fertility following progestin treatment and treatments to synchronize ovarian follicular development and estrus 下载免费PDF全文
Colazo MG Kastelic JP Small JA Wilde RE Ward DR Mapletoft RJ 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(1):49-56
The objective was to optimize rebreeding of nonpregnant, previously inseminated beef cattle. In Experiment 1, 43 cows received a used intravaginal progesterone-releasing insert (IVPRI; Days 0-7) 12.3 d after ovulation and received concurrently no treatment, 100 microg gonadotropin releasing hormone (GnRH), 1 mg estradiol cypionate (ECP), or 150 mg progesterone. Emergence of a new ovarian follicular wave was most synchronous (P < 0.0001) in the GnRH group. In Experiment 2, 675 heifers were given GnRH or no treatment on Day 0, fed melengestrol acetate (MGA; 0.5 mg/head/d) from Days 0-5 (Day 0 = 13-14 d after timed insemination; TAI), given 0.5 mg ECP or nothing on Day 7, and reinseminated 6-12 h after onset of estrus. Estrus was more synchronous (P < 0.05) in heifers given GnRH versus no treatment on Day 0. In Experiment 3, 317 TAI heifers were resynchronized with either MGA or a used IVPRI with or without ECP on Day 7; estrus was more synchronous (P < 0.05) and pregnancy rates were higher (54.1% versus 39.2%, P < 0.05) in heifers given a used IVPRI than those fed MGA. For resynchronization of heifers, pregnancy rates were not significantly improved with GnRH treatment, but were higher with a used IVPRI than with MGA. 相似文献
997.
998.
Effect of a 1800 MHz electromagnetic field emitted during embryogenesis on chick development and hatchability 下载免费PDF全文
K. Pawlak Z. Nieckarz A. Sechman D. Wojtysiak B. Bojarski B. Tombarkiewicz 《Anatomia, histologia, embryologia》2018,47(3):222-230
The level of artificial electromagnetic field (EMF) has steadily increased with the development of human civilization. The developing chicken embryo has been considered a good model to study the effects of EMF on living organisms. The aim of the study was to determine the effect of a 1800 MHz electromagnetic field during embryogenesis on the frequency of chick embryo malformations, morphometric parameters of the heart and liver and concentration of corticosterone in blood plasma, lipid and glycogen content in the liver of newly hatched chicks. A 1800 MHz EMF was found to shorten the duration of embryogenesis (earlier pipping and hatching of chicks) while having no effect on the quantity and quality of chicks and on increasing the incidence of embryo malformations. Exposure of chick embryos to EMF caused decreases in relative heart weight and right ventricle wall thickness. The pipping and hatching of chicks can be accelerated by stressful impact of EMF, which is confirmed by a significant increase in plasma corticosterone concentrations and decrease in fat and glycogen in the liver of chicks exposed during embryogenesis on the electromagnetic field with a frequency of 1800 MHz. 相似文献
999.
P Schmidely P Bas A Rouzeau J Hervieu P Morand-Fehr 《Journal of animal science》1992,70(11):3381-3390
The effects of anabolic agents (5 mg of estradiol-17 beta + 30 mg of trenbolone acetate) on body characteristics and chemical composition of gain were studied in 58 intact male goat kids fed milk replacer. Four kids were slaughtered at 7 d of age to constitute the initial group. The other kids were allotted in a 2 x 3 factorial arrangement with slaughter age (41, 49, and 56 d of age) and treatment (control and implanted at 21 d old) as factors. Energy intake decreased during the first 4 wk after treatment; implanted kids had the same ADG as controls but a better energy efficiency (P < .05). During the last week of the trial, energy intake was the same; treated kids tended (P < .10) to have a higher empty body gain (EBG). Anabolic agents increased carcass (P < .10) and hide proportions (P < .01) in empty BW and in EBG for all groups (except for carcass at 56 d old). Anabolic agents reduced the contribution of adipose tissues (P < .05), empty digestive tract (P < .05), and other organs (P < .01) to EBG. At all slaughter ages, the chemical composition of carcass and hide wet tissues, all dissectible adipose tissues, and EBG were altered by treatment. In these tissues (except for mesenteric fat tissue), water content increased and lipid content decreased (P < .05), but these effects diminished with age. Expressed on a DM basis, the CP content of the treated carcasses was increased in all groups (P < .05). Implanting high doses of steroids altered nutrient partitioning, which reduced fat and increased water and protein in the body. 相似文献
1000.
Lack of effect of recombinant bovine interferon alpha I1 in the treatment of experimentally-induced bovine warts. 下载免费PDF全文
Fifteen four-month old calves were inoculated, on five scarified sites on each side of the neck, with a suspension of ground wart tissue from a steer naturally infected with bovine papilloma virus 1. Warts started to appear about one month postinfection and were measurable in ten calves two months postinfection, when the trial started. After stratification on the size of the warts, all fifteen calves were allocated randomly to one of the following treatment groups: twice weekly intramuscular injections of 5 mg recombinant bovine interferon alpha I1 (rBoIFN alpha I1), weekly injection of 5 mg of rBoIFN alpha I1 or placebo, for three weeks. The biggest wart on each calf at the beginning of the trial was measured and photographs of all warts were taken weekly for five weeks. An analysis of covariance on the log of the volumes of warts during the five weeks of the trial showed a significant difference between groups (p = 0.026). Warts in treated groups tended to grow faster than in the placebo group. 相似文献