豫州褐壳蛋鸡Ⅰ,Ⅱ系杂交效果分析

对豫州褐壳蛋鸡Ⅰ、Ⅱ系杂交效果研究表明,其生活力、主要生产性能均有明显提高,健雏率、育雏率、育成率、产蛋期存活率分别达到９９．０２％,９７．８７％,９７．１２％和９３．３５％,开产日龄提早６￣９ｄ,全期产蛋２５４．２０枚,料蛋比２．４２：１。     

Identification of QTLs associated with salinity tolerance at late growth stage in barley

Salinity is a major abiotic stress to barley (Hordum vulgare L.) growth and yield. In the current study, quantitative trait loci (QTL) for yield and physiological components at the late growth stage under salt stress and non-stress environments were determined in barley using a double haploid population derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive). A total of 30 QTLs for 10 traits, including tiller numbers (TN), plant height, spikes per line (SPL), spikes per plant (SPP), dry weight per plant, grains per plant, grain yield, shoot Na⁺ (NA) and K⁺ concentraitions (K) in shoot, and Na⁺/K⁺ ratio (NAK), were detected, with 17 and 13 QTLs under non-stress and salt stress, respectively. The phenotypic variation explained by individual QTL ranged from 3.25 to 29.81%. QTL flanked by markers bPb-1278 and bPb-8437 on chromosomes 4H was associated with TN, SPL, and SPP under salt stress. This locus may be useful in the breeding program of marker-assisted selection for improving salt tolerance of barley. However, QTLs associated with NA, K, and NAK differed greatly between non-stress and salt stress environments. It may be suggested that only the QTLs detected under salt stress are really associated with salt tolerance in barley. D. Xue and Y. Huang contributed equally to the article.     

DNA profiling and genetic diversity of Korean soybean (<Emphasis Type="Italic">Glycine max</Emphasis> (L.) Merrill) landraces by SSR markers

Approximately 7,000 accessions of Korean soybean (Glycine max (L.) Merrill) landraces, largely composed of three collections, the Korea Atomic Energy Research Institute’s soybean (KAS), the Korean Crop Experiment Station’s soybean (KLS) and the Korean Agricultural Development and Technology Center’s soybean (KADTC) collections, have been conserved at the Rural Development Administration (RDA) genebank in Korea. The accessions within collections were classified based on their traditional uses such as sauce soybean (SA), sprouted soybean (SP), soybean for cooking with rice (SCR), and OTHERS. A total of 2,758 accessions of Korean soybean landraces were used to profile and to evaluate genetic structure using six SSR loci. A total of 110 alleles were revealed by at the six SSR loci. The number of alleles per SSR locus ranged from 9 to 39 in Satt187 and Satt_074, respectively. The number of alleles ranged from 87 in the KADTC collection to 96 in the KLS collection, and from 63 in the SCR group to 95 in the SP group. Nei’s average genetic diversity ranged from 0.68 to 0.70 across three collections, and 0.64 to 0.69 across the usage groups. The average between-group differentiation (G _st) was 0.9 among collections, and 4.1 among the usage groups. The similar average diversity among three collections implies that the genetic background of the three collections was quite similar or that there were a large number of duplicate accessions in three collections. The selection from the four groups classified based upon usage may be a useful way to select accessions for developing a Korean soybean landrace core collection at the RDA genebank. DNA profile information of accessions will provide indications of redundancies or omissions and aid in managing the soybean collection held at the RDA genebank. The information on diversity analysis could help to enlarge the genetic diversity of materials in breeding programs and could be used to develop a core collection.     

磷酰胺除草剂APM诱导洋葱根尖分生组织细胞异常有丝分裂和蛋白质组分变化

研究了不同浓度APM(4～6 μmol/L)和不同处理时间(4～6 h)对洋葱根尖细胞有丝分裂的影响.结果表明,当用5.6 μmol/L APM处理洋葱分生组织16 h后,中期细胞有丝分裂指数(Met.Ⅰ)从0.8(对照)分别提高至5.0、5.2.结果也同时表明,当APM浓度超过4 μmol/L时,严重影响细胞有丝分裂.在根尖细胞中,多极分裂细胞(尤其是3～4级),中期染色     

转盘式对虾开背工艺分析与参数优化

针对目前对虾开背环节工艺不完善、实际应用装备缺乏等问题,以中型南美白对虾为研究对象,探究了对虾开背关键工艺方法,建立了对虾开背过程力学模型,获得影响开背效果主要因素为刀具角度、加工中心盘转速和刀具安置距离;采用单因素试验研究和分析了各因素对开背成功率、虾仁损伤率、虾线裸露成功率和感官评分值的影响,确定影响因素最佳区间为：刀具角度20°~60°、加工中心盘转速10~40r/min和刀具安置距离9~11mm;利用Design-Expert软件进行多因素响应面试验研究分析,并采用多目标优化方法,得到最优参数组合为：刀具角度43.838°、加工中心盘转速28.391r/min、刀具安置距离9.801mm时,开背成功率为99.161%、虾仁损伤率为2.825%、虾线裸露成功率为90.727%、感官评分值为86.944。验证试验结果表明：针对中型南美白对虾,当刀具角度45°、加工中心盘转速28r/min、刀具安置距离9.8mm时,开背成功率为98.89%、虾仁损伤率为3.33%、虾线裸露成功率为87.78%、感官评分值为85.33,与理论优化值的绝对误差均较小,优化后的对虾开背装置性能满足作业要求。研究可为对虾开背工序装置设计和参数选择提供理论基础和科学依据。     

灌溉施氮和种植密度对棉花叶面积指数与产量的影响

为构建便于实际应用的棉花叶面积指数和产量模型,综合考虑灌溉、施肥、种植密度及覆膜的影响,建立了基于相对有效积温的普适棉花相对叶面积指数的Logistic模型,并研究了棉花最大叶面积指数与全生育期灌水量、施氮量、种植密度及产量之间的关系。结果表明：覆膜棉花叶面积指数最大时的有效积温为1400℃左右,不覆膜棉花的叶面积指数最大时的有效积温为1600℃左右。棉花最大叶面积指数随耗水量、施氮量呈现出先增后减的变化趋势,而种植密度与最大叶面积指数之间表现出明显的正相关性。综合考虑灌水量、施氮量和种植密度的作用可以较为准确描述最大叶面积指数变化特征。棉花产量随着最大叶面积指数的变化呈现出明显的先增后减的变化趋势,当最大叶面积指数为4.93时籽棉产量最大达6066.2kg/hm2。相对化分析结果表明,覆膜与不覆膜棉花相对叶面积指数的变化趋势基本一致,相应的模型参数也基本相同,从而建立了全国范围内覆膜与不覆膜棉花统一的相对叶面积指数的Logistic模型。该研究为棉花科学种植和精细化管理提供了方法,也为其他作物在不同管理措施和地域时进行建模提供了参考。     

八轮四摆臂无人机动平台越障性能分析与试验

针对南方山区丘陵地带地形复杂、传统农田运输车辆通过性不足的问题,提出并设计了一种具有仿生液压驱动摆臂机构的八轮无人机动平台,其车体姿态可通过四摆臂协同动作进行调节,以适应不同形式地面障碍。越障性能是制约平台通过性的根本因素,建立了无人平台姿态规划模型和关键越障过程动力学模型,得到无人平台在典型垂直障碍的越障性能。为验证理论分析,在ADAMS建立了二次开发仿真平台,并进行了样机动力性试验。研究表明,八轮四摆臂无人机动平台可攀爬高度为轮胎直径1. 13倍的垂直障碍,具有良好的复杂地面环境通过能力,可满足丘陵地带农用运输车辆在复杂农田地形的行走需求。     

冬小麦叶片水势、气孔阻力、蒸腾速率与环境因素的关系

本文依据田间试验资料,分析了冬小麦叶片水势、气孔阻力和蒸腾速率在不同土壤基质势条件下的日变化规律,探讨了其与土壤基质势和天气条件(光照强度、净辐射、饱和差、气温)之间的关系,建立了叶片水势、气孔阻力、蒸腾速率与环境因素间的定量关系式。     

Variability in energy partitioning and resistance parameters for a vineyard in northwest China

Grapevines are extensively grown in the semiarid and arid regions, but little information is available on the variability of energy partitioning and resistance parameters for the vineyard. To address this question, an eddy covariance system was applied to measure energy balance over a vineyard in northwest China during 2005-2006. Result indicated that 2-year average Bowen ratio (β) of vineyard was 1.0, canopy resistance (r_c) 289.3 s m⁻¹, aerodynamic resistance (r_a) 9.7 s m⁻¹ and climatological resistance (r_i) 117 s m⁻¹. This implied that the annual energy was split almost equally between sensible heat and latent heat. Compared to the corresponding values in other ecosystems reported by Wilson et al. [Wilson, K.B., Baldocchi, D.D., Aubinet, M., Berbigier, P., Bernhofer, C., Dolman, H., Falge, E., Field, C., Goldstein, A., Granier, A., Grelle, A., Halldor, T., Hollinger, D., Katul, G., Law, B.E., Lindroth, A., Meyers, T., Moncrieff, J., Monson, R., Oechel, W., Tenhunen, J., Valentini, R., Verma, S., Vesala, T., Wofsy, S., 2002. Energy partitioning between latent and sensible heat flux during the warm season at FLUXNET sites. Water Resource Research 38, 1294-1305.], the vineyard had a higher β, r_c and r_i than deciduous forests, corn and soybean, and grassland. Such difference was mainly attributed to (1) serious water stress in 2005, which resulted in a greater r_c up to 364.4 s m⁻¹; (2) sparse canopy with row spacing of 2.9 m and plant spacing of 1.8 m; (3) warm-dry climate and high attitude (1581 m) along with higher r_i and lower psychrometer (54 Pa K⁻¹) in the arid region of northwest China. These characters of vineyard revealed varying process of energy partitioning and surface resistance, and provided a scientific basis in understanding and modeling water and energy balance for the vineyard in the semiarid and arid regions.