Effect of more stringent convergence criterion of estimated breeding values on response to selection

In the analysis of large amounts of data to obtain BLUP, large sets of mixed model equations must be solved iteratively, which can involve considerable computing time. In real life, solutions are required only periodically for breeders to choose the best individuals, so that computing time is not usually a limiting factor. In simulation studies involving evaluation of individuals by BLUP, many rounds of evaluation are required for each simulated population. Since several or many replicates are usually required to obtain an accurate result from stochastic simulations, computing time can become a limiting factor. One of the factors that can drastically affect computing time in iterative methods is the criterion for ceasing iteration, or convergence criterion (CC). With iterative methods, a disadvantage can be that the rate of convergence can be slow, or under certain circumstances not converge at all. Nevertheless, when the system converges, the more stringent the CC, the more accurate the solutions. The more stringent the CC the more iterations and hence more computing time is required. The objectives of this study were to investigate how much response to selection is affected by the stringency of the CC and how much reranking occurs among selected individuals at different levels of the convergence criteria. These explorations provided a profile analysis of the computing time spent for each of the major subroutines in the BBSIM program.     

Microsatellite variation between an African and five European taurine breeds results in a geographical phylogenetic tree with a bison outgroup

Genetic variation is being used extensively for individual identification and linkage analysis, and may be useful for interpopulation studies. Previously, blood groups and biochemical variants in blood cell and serum proteins have been used to study (evolutionary) relationships in mammals. But genetic divergence and gene flow among closely related populations are difficult to measure with these classical markers because their mutation rate is so low that new mutations have not had sufficient time to appear and become fixed. So they have a small number of alleles and a relatively low level of heterozygosity. These markers are now replaced by DNA markers, mostly microsatellites. These microsatellite loci are useful genetic markers at which alleles differ in length due to differences in the number of short sequence motifs arranged adjacent to one another. They are abundantly distributed throughout the mammalian genome. They have a large number of alleles, a high level of heterozygosity and are inherited in true Mendelian fashion. These characteristics make them valuable for parentage control, linkage analysis, genome mapping and phylogenetic studies. In terrestrial vertebrates with limited mobility, genetic differentiation often increases with the distance between populations or corresponds to the extent of geographic and habitat barriers (R oy et al. 1994). Investigations of short tandem repeats yield a considerable volume of genetic data regarding the similarities and divergence times of different cattle populations. Microsatellite markers are suitable for the estimation of these parameters as they are not generally subject to direct selection and environmental influences. Computation of genetic distances based on data from several loci can be used to evaluate the taxonomic relationship between populations. The aim of this study was to estimate the relative genetic variability between Belgian cattle breeds and to reconstruct the evolutionary relationship among them, also using two small genetically isolated cattle-like populations.     

Homeostatic adjustments of iodine metabolism and tissue iodine to widely varying iodine supply in 125I labeled rats

Introduction   The homeostatic regulation of nutrient flow through the body is a fundamental ability of living organisms and has been shown to be active also in the case of several trace elements (K irchgessner 1993; K irchgessner et al. 1997). The basic function of homeostasis is to maintain the body's internal status of the trace elements within physiologically tolerable margins by controlling at least one of the major trace element fluxes: the true absorption of the trace elements from the diet into the body, the reflux from the body into the faeces (endogenous faecal excretion), and the urinary excretion. Furthermore iodine may be a candidate to homeostatic regulation since dietary iodine contents may vary over a wide range and it may be important to the organism to maintain a constant internal status also of this essential trace element. However, quantitative iodine balance measurements are hardly available from the literature and thus knowledge about the existence of iodine homeostasis and its mode of action is still fragmentary. An additional problem also was the lack of appropriate methods to quantify true absorption and endogenous faecal excretion of iodine. However, a recent study has overcome this methodological barrier by adapting the isotope-dilution technique to iodine (W indisch et al. 1999). Therefore, the aim of the present experiment was to quantify possible homeostatic adaptations of iodine metabolism to dietary iodine ranging from deficient to excessive supplies and to measure the interactions to tissue iodine.     

铜基木材防腐剂

铜是大部分活细胞的基本微量元素之一,其剂量较大时,铜离子有杀虫灭菌功效。铜基防腐剂已广泛应用了一个多世纪。虽然硼酸盐和有机防腐剂受到了重视,铜仍然是与土壤接触和完全暴露在大气中的木材所用防腐剂的主要成分。铜化合物的显著优点是,较容易制成水载或水溶性药剂;较容易测出渗入木材的深度;并能减缓紫外线或水引起的木材光降解。     

Breed differences and genetic parameters for melting point, marbling score and fatty acid composition of lot-fed cattle

Fatty acid composition, marbling score and melting point data collected between 1994 and 1996 were analysed. The data were from the adipose tissue of 764 Angus, Belgian Blue, Hereford, Jersey, Limousin, South Devon and Wagyu crossbred cattle slaughtered after lot‐feeding at 500 days of age. The aim was to investigate sire‐breed differences and to estimate heritability and genetic and phenotypic correlations. Significant breed differences were found: Jersey crosses had the highest marbling score and Belgian Blue crosses had the lowest. Limousin crosses had the highest melting point and Jersey crosses the lowest. South Devon crosses had the highest proportion of stearate and Jersey crosses the lowest. Desaturation indices in C16 and C18 fatty acids were highest in Jersey crosses and lowest in Limousin and South Devon crosses. In contrast, there were no breed differences in the proportions of palmitate, oleate, total saturated, total mono‐unsaturated fatty acids and elongation index. Heritability estimates of individual fatty acids and their summations, melting point and marbling were low to moderately low (0.05–0.27). Strong genetic correlations of melting point and desaturation index in C16 fatty acids (‐0.93), melting point and stearate (0.62), marbling and stearate (‐0.71) and marbling and desaturation index in C18 fatty acids (0.62) were observed. Phenotypic correlation were generally low. The results imply that fatty acids in the adipose tissue of lot‐fed cattle have a moderately low heritability, hence genetic progress might be slow.     

UV‐B radiation constrains the photosynthesis of Quercus robur through impacts on the abundance of Microsphaera alphitoides

Quercus robur saplings were exposed at an outdoor facility in the UK to supplemental levels of UV‐B radiation (280–315 nm) under arrays of cellulose diacetate‐filtered fluorescent lamps which also produced UV‐A radiation (315–400 nm). Saplings were also exposed to supplemental UV‐A radiation under arrays of polyester‐filtered lamps and to ambient levels of solar radiation under arrays of unenergized lamps. The UV‐B treatment was modulated to maintain a 30% elevation above the ambient level of erythemally weighted UV‐B radiation. Naturally occurring infections by oak powdery mildew (Microsphaera alphitoides) were more abundant, and developed more rapidly, on lammas leaves of saplings which were exposed to treatment levels of UV‐B radiation than on leaves of saplings exposed to supplemental UV‐A or to ambient levels of solar radiation over 12 weeks in summer and autumn 1996. An analysis of leaf photosynthetic capacities revealed that M. alphitoides infection reduced the quantum efficiency of photosystem (PS) II by 14% at moderate irradiance. Although there was no direct effect of UV‐B radiation on PSII photochemistry, exposure of saplings to supplemental UV‐A radiation under polyester‐filtered lamps resulted in a 17.5% decrease in PSII quantum efficiency, compared with saplings exposed to ambient solar radiation. The results from our study suggest that photosynthesis of Q. robur may be constrained by exposure to UV‐B radiation in the natural environment through impacts on the abundance of M. alphitoides.     

Effect of inoculum density and borate concentration in a stump treatment trial against Heterobasidion annosum

The effectiveness of disodium octaborate tetrahydrate (DOT) as a stump treatment chemical for Sitka spruce in Britain was tested on six occasions by inoculating treated and untreated stumps with basidiospores of Heterobasidion annosum at three concentrations ranging from an average 49 viable spores/ml to 4.9 × 10⁵/ml of water. The extent of colonization of heartwood by H. annosum was measured and, along with the incidence of infected stumps, provided a measure of the combined effects of spore concentration and of the two DOT treatments (15 and 30 g/m²) on the trial results. On untreated stumps, both the incidence of infection and the cross‐sectional area of stump heartwood colonized by the fungus increased with inoculum density. The same effect was evident in treated stumps, but it was reduced by increasing DOT application. Infection was at its lowest in stumps treated with DOT at 30 g/m², being entirely absent from those 60 stumps that were inoculated with the fewest spores. The implications of these findings for the design of trials of control agents that rely on artificial inoculation with H. annosum and for the selection of dose rates to use in harvesting operations are discussed.     

Effects of simulated acid deposition on the surface structure of Norway spruce and silver fir needles

Treatments with acidified solutions of pH 3.5 cause alterations in the wax structure of the needles of silver fir and Norway spruce. The wax structures, especially those of the stomata, once they have been damaged, are not capable of complete reorganization even if the needle is repeatedly treated with distilled water.