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41.
Ngure RM Eckersall PD Jennings FW Mburu J Burke J Mungatana N Murray M 《Veterinary parasitology》2008,151(1):14-20
Mice infected with Trypanosoma congolense developed a severe anaemia 1 week after infection, which persisted till treatment with diminazine aceturate when the packed cell volume (PCV) recovered to pre-infection levels. This was accompanied by a marked increase in the plasma levels of the acute phase proteins (APP), serum amyloid P-component (SAP) and haptoglobin (Hp). The initial peak levels of Hp and SAP were attained 7 and 12 days post-infection (DPI), respectively. Thereafter SAP levels decreased significantly to near pre-infection levels, but later increased even after treatment to give a second peak 34 DPI after which there was a decline till the study was terminated. The Hp levels on the other hand decreased to an intermediate level after the initial peak increasing to a second peak 22 DPI. Thereafter Hp decreased significantly following diminazine aceturate treatment to reach pre-infection levels within 5 days post-treatment. This indicates that T. congolense-infected mice develop severe anaemia accompanied by an acute phase response leading to an increase in SAP and Hp but that following treatment divergent responses occurred indicating differences in the pathways for stimulation of the APP. Haptoglobin was shown to be an earlier indicator of infection and a better marker in monitoring the response to treatment. 相似文献
42.
43.
F. Burke M.A. O''Donovan J.J. Murphy F.P. O''Mara F.J. Mulligan 《Livestock Science》2008,114(2-3):325-335
Sixty cows (40 multiparous and 20 primiparous) averaging 140 days in milk (DIM) were assigned to five treatments to evaluate the effect of pasture allowance and supplements of a) maize silage b) high crude protein concentrate and, c) low crude protein concentrate on milk production and nitrogen (N) excretion. Two of the treatments (HG and LG) were offered herbage only (allowances of 20 and 15 kg dry matter (DM) per cow/day, respectively) while the remaining three treatments were offered a herbage allowance of 15 kg DM per cow/day plus 4 kg DM per cow/day of maize silage (M), a high CP concentrate (CP concentration of 194 g/kg DM) (HC), or citrus pulp (CP concentration of 69 g/kg DM) (LC). Total DM intake (DMI) was similar for HG, M, HC, and LC but was lower for LG (15.2 kg DM per cow/day) than HC (17.4 kg DM per cow/day). The reduction in pasture DMI per kg of supplement DM ingested was 0.44, 0.45, and 0.54 kg for cows offered maize silage, high CP concentrate, and low CP concentrate, respectively. Milk yield was greater for the supplemented treatments (23.7–24.7 kg/day) than for LG (20.7 kg/day) but not for HG (23.2 kg/day). Milk fat concentration was greater for HC (35.3 g/kg) than for HG, M, and LC but not greater than LG, while milk protein concentration was greater for HG (34.8 g/kg) than for LG and HC but not greater than M and LC. The greatest levels of N and PDIN intake were recorded for HG (662 and 2502 g/day) and HC (654 and 2506 g/day) which were greater than LC but not greater than LG and M. Treatment HC recorded the highest PDIE intake (1743 g/day) which was greater than LG, M and LC but not greater than HG. Output of N in milk was greater on HC (134 g/day) than on LG but was not greater than on HG, M, and LC. Faecal N excretion was greater on HG (171 g/day) than on all other treatments while estimated urinary N excretion was greater on HG and HC than LC (320 g/day). Treatment LC had a higher proportion of N output in milk (0.23) than treatment LG but not higher than HG, M and HC treatments. Urinary N expressed as a proportion of total N excretion was lower for HG (0.68) than all other treatments. The results of this study show that there is a large response in milk production to supplementing cows on a restricted grass allowance and that cows offered low CP supplements had similar levels of production to those offered high CP supplements. Nitrogen utilisation was improved by offering supplements of lower CP content. 相似文献
44.
Copper oxide wire particles (COWP) have been used to reduce infection of Haemonchus contortus in hair breed lambs in southeastern USA without signs of copper toxicity. However, copper sensitivity among breeds and regions varies. The objective was to determine the effectiveness and safety of COWP in lactating Polypay ewes and their offspring grazing alfalfa/bluegrass pasture in a rotational grazing system. Mature Polypay ewes were administered 0, 0.5, 1, or 2 g (n=8 or 9/dose) COWP approximately 60 days after lambing in mid-July 2005. Their offspring were administered 0 (n=6), 0.5 or 0.75 g (n=9), 1 or 2 g (n=6) COWP 2 weeks later in late July. The primary gastrointestinal nematode was H. contortus (70%). Between Days 7 and 35, FEC were greater in 0 and 0.5 g COWP groups compared with ewes administered 2 g COWP (COWP x day, P<0.004). PCV decreased in all groups of ewes between Days 0 and 21 (day, P<0.001). Aspartate aminotransferase (AST) activity, a measure of liver copper levels, and body weight was similar among groups of ewes. FEC decreased within 7 days in COWP-treated compared with untreated lambs and remained low throughout experiment (COWP x day, P<0.05). PCV increased in COWP-treated lambs between Days 7 and 35 and decreased in untreated lambs between Days 0 and 21 (COWP x day, P<0.009). AST activity was similar among groups of lambs. Administration of 2 g COWP to ewes prevented a rise in FEC, but a dose of 0.5 g was ineffective as an anthelmintic. Administration of all doses of COWP to lambs decreased FEC and increased PCV compared to untreated lambs. There were no signs of copper toxicity in ewes or lambs. Alternative suppression of H. contortus infections may be necessary in ewes, but COWP was effective in H. contortus management for lambs. 相似文献
45.
R. A. C. Mitchell C. R. Black S. Burkart J. I. Burke A. Donnelly L. de Temmmerman A. Fangmeier B. J. Mulholland J. C. Theobald M. van Oijen 《European Journal of Agronomy》1999,10(3-4):205-214
Spring wheat cv. Minaret crop stands were grown under ambient and elevated CO2 concentrations at seven sites in Germany, Ireland, the UK, Belgium and the Netherlands. Six of the sites used open-top chambers and one used a controlled environment mimicking field conditions. The effect of elevated CO2 for a range of N application regimes, O3 concentrations, and growth temperatures on flag leaf photosynthesis was studied. Before anthesis, flag leaf photosynthesis was stimulated about 50% by 650 compared with 350 μmol mol−1 CO2 at all sites, regardless of other treatments. Furthermore, there was no evidence of a decrease in photosynthetic capacity of flag leaves due to growth at elevated CO2 before anthesis, even for low N treatments. However, photosynthetic capacity, particularly carboxylation capacity, of flag leaves was usually decreased by growth at elevated CO2 after anthesis, especially in low N treatments. Acclimation of photosynthesis to elevated CO2 therefore appears to occur only slowly, consistent with a response to changes in sink–source relationships, rather than a direct response. Effect of elevated CO2 on stomatal conductance was much more variable between sites and treatments, but on average was decreased by ˜10% at 650 compared with 350 μmol mol−1 CO2. Carboxylation capacity of flag leaves was decreased by growth at elevated O3 both before and after anthesis, regardless of CO2 concentration. 相似文献
46.
Responses of the two‐spotted oak buprestid,Agrilus biguttatus (Coleoptera: Buprestidae), to host tree volatiles
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47.
M. Abdalla K. Rueangritsarakul M. Jones B. Osborne M. Helmy B. Roth J. Burke P. Nolan P. Smith M. Williams 《Water, air, and soil pollution》2012,223(8):5155-5174
Field management is expected to influence nitrous oxide (N2O) production from arable cropping systems through effects on soil physics and biology. Measurements of N2O flux were carried out on a weekly basis from April 2008 to August 2009 for a spring sown barley crop at Oak Park Research Centre, Carlow, Ireland. The soil was a free draining sandy loam typical of the majority of cereal growing land in Ireland. The aims of this study were to investigate the suitability of combining reduced tillage and a mustard cover crop (RT?CCC) to mitigate nitrous oxide emissions from arable soils and to validate the DeNitrification?CDeComposition (DNDC) model version (v. 9.2) for estimating N2O emissions. In addition, the model was used to simulate N2O emissions for two sets of future climate scenarios (period 2021?C2060). Field results showed that although the daily emissions were significantly higher for RT?CCC on two occasions (p?<?0.05), no significant effect (p?>?0.05) on the cumulative N2O flux, compared with the CT treatment, was found. DNDC was validated using N2O data collected from this study in combination with previously collected data and shown to be suitable for estimating N2O emissions (r 2?=?0.70), water-filled pore space (WFPS) (r 2?=?0.58) and soil temperature (r 2?=?0.87) from this field. The relative deviations of the simulated to the measured N2O values with the 140?kg N ha?1 fertiliser application rate were ?36?% for RT?CCC and ?19?% for CT. Root mean square error values were 0.014 and 0.007?kg N2O?CN ha?1 day?1, respectively, indicating a reasonable fit. Future cumulative N2O fluxes and total denitrification were predicted to increase under the RT?CCC management for all future climate projections, whilst predictions were inconsistent under the CT. Our study suggests that the use of RT?CCC as an alternative farm management system for spring barley, if the sole objective is to reduce N2O emissions, may not be successful. 相似文献
48.
Burke JM Whitley NC Pollard DA Miller JE Terrill TH Moulton KE Mosjidis JA 《Veterinary parasitology》2011,181(2-4):345-349
The objective of three experiments was to determine the impact of supplementing sericea lespedeza (Lespedeza cuneata; SL) in three concentrations in a loose or pelleted diet on gastrointestinal nematodes (GIN) in small ruminants. Experiments on lambs were conducted at the USDA, Agricultural Research Service in Booneville, AR (Exp. 1) and at Louisiana State University in Baton Rouge, LA (Exp. 2); an experiment on goat kids occurred at University of Maryland-Eastern Shore (Exp. 3). Exp. 1 used crossbred hair sheep lambs naturally infected with GIN that were randomly allocated to diets containing 0, 25, 50, and 75% SL diets (n=11 or 12/diet). Exp. 2 consisted of Haemonchus contortus-inoculated crossbred wool breed lambs that were blocked by gender and FEC and randomly assigned to 0, 25, 50, or 75% SL diet (n=8/diet). Fecal egg counts (FEC) and blood packed cell volume (PCV) were not influenced by SL supplementation in Exp. 1 and 2. Exp. 3 consisted of naturally GIN infected Boer crossbred goat kids in individual pens. Kids were blocked by FEC and randomly allotted to treatments of 0, 20, 40, or 60% SL with 9-13 goats/diet. The more SL fed, the greater the reduction in FEC (P<0.001). There was an increase in PCV in SL fed goats (P<0.001). Larval speciation at the end of the experiment indicated that feces from control animals produced 43% H. contortus larva while 20, 40 and 60% SL resulted in 39%, 35% and 31% H. contortus larvae, respectively. Feeding dried SL may be less effective in lambs than kids, though concurrent studies must be conducted to confirm this. 相似文献
49.
Control of gastrointestinal nematodes (GIN) in small ruminants in regions of the world where anthelmintic resistance is prevalent must rely on more than just chemical deworming strategies. The objective of this experiment was to compare two integrated treatment protocols for control of GIN (primarily Haemonchus contortus in this region) using anthelmintics, copper oxide wire particles (COWP) and FAMACHA(?), compared to traditional anthelmintic use only. Three separate trials were conducted on mature ewes, weaned goats, and weaned lambs in which three deworming management strategies were applied: 1) all animals were dewormed with levamisole at four week intervals (LEV), 2) individual animals were dewormed with moxidectin when scored≥4 (ewes) or ≥3 (kids and lambs) using FAMACHA(?) (FAM/MOX), 3) all animals were dewormed with moxidectin initially and again with COWP (2g) when group mean FEC exceeded 500 (ewes), 3000 (kids), or 1000 (lambs) eggs/g (MOX/COWP). In this final group, during periods between group treatments, individual animals were dewormed with albendazole and levamisole according to FAMACHA(?) score. Fecal egg counts (FEC) and blood packed cell volume (PCV) were determined every 7 days and body weight every 28 days for 30, 20, or 16 weeks in ewes, goats, and lambs, respectively. Efficacy of levamisole was 83.4-86.4%, efficacy of moxidectin was 93.5-100%, and efficacy of COWP was 10.8-98.1% among the three trials. The mean number of deworming treatments per animal that occurred for the LEV, FAM/MOX, and MOX/COWP groups, respectively, was 7.9, 0.6, and 2.5±0.4 (P<0.001) for ewes, 5.2, 1.6, and 3.4±0.3 (P<0.001) for goats, and 4.0, 1.7, and 3.6±0.2 (P<0.001) for lambs. Production (body weight of lambs weaned from ewes or final body weight of kids and lambs) was similar among management strategy groups for ewes and kids, but FAM/MOX lambs were lighter by the end of the trial (P<0.003). While more time and labor was required to use the FAMACHA(?) system, a more conservative use of anthelmintics occurred in the FAM/MOX group. 相似文献
50.
David J. Burke Shun Zhu Michele P. Pablico-Lansigan Charlotte R. Hewins Anna Cristina S. Samia 《Biology and Fertility of Soils》2014,50(7):1169-1173
We examined the effect of TiO2 nanoparticles (NPs) on the growth of maize and soybean plants and associated soil microbial communities. Plants were grown in a greenhouse, and low levels of undoped or nitrogen-doped TiO2 NPs were applied. Plant growth and nutrient content were determined, and effects of NPs on composition of soil microbial communities were examined using terminal restriction fragment length polymorphism analysis (TRFLP) of rDNA. We found no significant effects of TiO2 NPs on plant growth, nutrient content, or the composition of bacterial communities within the rhizosphere. However, arbuscular mycorrhizal fungal communities were affected by application of undoped and nitrogen-doped TiO2 NPs. This observation may be partially attributed to the small but significant TiO2 NP uptake levels in the root tissues of both plants. Our results suggest that even low concentrations of TiO2 NPs may influence some important groups of soil microbes, such as mycorrhizal fungi, but changes in the composition of microbial communities may not affect plant growth under conditions of adequate moisture and nutrients. 相似文献